EST-SSR mark for distinguishing different ecotypes of cloves and applied primers

An ecological and clove technology, applied in the biological field, can solve the problems of complicated experimental process, time-consuming and labor-intensive, low efficiency, etc.

Active Publication Date: 2019-07-09
BEIJING UNIV OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

gSSR is located in the coding region and non-coding region of the genome, and the development of markers is mainly based on the method of gene library co

Method used

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  • EST-SSR mark for distinguishing different ecotypes of cloves and applied primers
  • EST-SSR mark for distinguishing different ecotypes of cloves and applied primers

Examples

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Embodiment 1

[0063] Embodiment 1, for distinguishing the EST-SSR mark of different ecotype cloves

[0064] This example provides a set of EST-SSR markers that can be used to distinguish different ecotypes of Syringa, and a set of EST-SSR primers that can amplify the set of EST-SSR markers. The primer information is shown in Table 1.

[0065] Table 1. 25 pairs of polymorphic EST-SSR primer information

[0066]

[0067]

[0068] The primers with odd numbers in Table 1 are forward primers, and the primers with even numbers are reverse primers. The 10 μL reaction system for PCR amplification of each pair of primers is: template (25ng / μL) 1 μL, 2×Power Taq PCR MasterMix (Beijing Aidelai Biotechnology Co., Ltd., Cat. No.: PC09) 5 μL, forward and reverse primers 0.5 μL each (concentration of forward and reverse primers in the reaction system are both 10 μM), ddH 2 O 3 μL. The templates used are the genomic DNA of clove leaves.

[0069] The PCR amplification reaction conditions were: pre-...

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Abstract

The invention discloses an EST-SSR mark for distinguishing different ecotypes of cloves and applied primers. The EST-SSR mark for distinguishing different ecotypes of cloves is obtained by conductingPCR amplification on a primer set composed of 50 single-chain DNAs shown in sequences 1-50 in a sequence table. The primer set can be applied to identification of allele polymorphism, identification of identical or related plants, plant distinguishing and research on the genetic diversity in the population. The primer set and the EST-SSR mark can be well applied to the clove category, and can be applied to distinguishing of different ecotypes of cloves in the clove category and provide convenience for development of auxiliary cultivation of molecular markers of the cloves and the sibling species of the cloves.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to EST-SSR markers and primers used for distinguishing different ecotypes of cloves. Background technique [0002] Clove (Syringa) is the general designation of perennial woody plants of the family Oleaceae (Oleaceae) Syringa (Syringa). It has been cultivated in China for more than 1,000 years and is a famous and precious flower in China. There are about 27 species of Syringa in the world, and about 22 species of Syringa in my country, 18 of which are endemic. The distribution of cloves in China is mainly concentrated in the southwest, northwest, north and northeast regions. Among them, there are 13 kinds of cloves in Sichuan, Yunnan and Tibet, which are also the places with the most endemic species of cloves in my country. Lilac is famous for its strong floral fragrance. Its plants are elegant and beautiful, its flowers are colorful, and its inflorescenc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13Y02A50/30
Inventor 吴静冷平生郑健胡增辉杨云尧何芮庆
Owner BEIJING UNIV OF AGRI
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