Application of tiRNA as drug target in metastasis treatment of colorectal cancer
A colorectal cancer and therapeutic target technology, applied in the field of biomedicine, can solve problems such as unclear mechanism of action and clinical significance
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Embodiment 1
[0113] Example 1: Expression of tiRNA in cancer tissues of patients with colorectal cancer
[0114] 1. Analysis of differential expression of tiRNA in paracancerous tissue and cancer tissue of colorectal cancer patients
Embodiment approach
[0115] Implementation method: The small non-coding RNA high-throughput sequencing method was used to detect the expression of tiRNAs in the paracancerous tissues and cancer tissues of 3 patients with colorectal cancer, and then the differentially expressed tiRNAs were analyzed by bioinformatics.
[0116] Result analysis: if figure 1It was shown that, compared with paracancerous tissues, 5'-tiRNAs significantly highly expressed in colorectal cancer tissues included 5'-tiRNA-Val-AAC, 5'-tiRNA-Ala-AGC, 5'-tiRNA-Pro-AGG , 5'-tiRNA-Val-CAC, 5'-tiRNA-Leu-CAG, 5'-tiRNA-Glu-CTC and 5'-tiRNA-Cys-GCA; significantly lower expressed 5'-tiRNAs include 5'- tiRNA-Gly-TCC, 5'-tiRNA-His-GTG, 5'-tiRNA-SeC-TCA and 5'-tiRNA-Tyr-GTA; significantly high expressed 3'-tiRNA including 3'-tiRNA-Ala- TGC, 3'-tiRNA-Ala-CGC, 3'-tiRNA-Gly-CCC, 3'-tiRNA-Val-TAC, 3'-tiRNA-Ala-AGC, and 3'-tiRNA-Met-CAT; low significance The expressed 3'-tiRNAs included 3'-tiRNA-Thr-TGT, 3'-tiRNA-Trp-CCA, 3'-tiRNA-Arg-ACG an...
Embodiment 2
[0121] Example 2: tiRNA can promote the metastasis and invasion ability of colorectal cancer cells
[0122] 1. Screening of colorectal cancer cell lines with high and low migration and invasion ability
[0123] Implementation method: Based on the Transwell migration and invasion assay, the colorectal cancer cells (HCT116) in the upper and lower layers of the Transwell chamber were isolated and cultured, and the isolation and culture method was based on the existing report MiR-218 inhibits invasion and metastasis of gastric cancer by targeting the Robo1receptor (PLoS Genet 2010; 6: e1000879); after 3 rounds of screening (the screening condition is whether the cells can migrate to the lower layer of the Transwell chamber), the cells that meet the conditions of migrating to the lower layer of the Transwell chamber and staying in the upper layer of the Transwell chamber are selected to screen and establish two different migration and invasion abilities. The sub-cell lines were nam...
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