Adapter mediated adenovirus targeting drug-delivery system and structuring method thereof

A technology of targeted drug delivery and construction method, which is applied in the field of aptamer-mediated adenovirus targeted drug delivery system and its construction, can solve problems such as limiting the application of aptamer, and achieves improved serum stability, enhanced targeting, The effect of prolonging the cycle time

Inactive Publication Date: 2017-07-21
SOUTHWEST MEDICAL UNIVERISTY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In the blood, aptamers are rapidly degraded by serum nucleases, which greatly limits the clinical application of

Method used

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  • Adapter mediated adenovirus targeting drug-delivery system and structuring method thereof
  • Adapter mediated adenovirus targeting drug-delivery system and structuring method thereof
  • Adapter mediated adenovirus targeting drug-delivery system and structuring method thereof

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Embodiment Construction

[0041] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0042] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0043] The aptamer-mediated adenovirus targeted drug delivery system provided in the examples of the present invention uses PEG as a linker to develop a new EpDT3-modified Ad5-PTEN gene drug delivery system EpDT3-PEG-Ad5-PTEN (EPAP).

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Abstract

The invention belongs to the technical field of a biotech drug, and discloses an adapter mediated adenovirus targeting drug-delivery system and a structuring method thereof; the structuring method includes steps of taking PEG as a truss arm, and applying EpDT3 modified Ad5-PTEN new gene drug-delivery system EpDT3-PEG-Ad5-PTEN (EPAP); performing amplification, purification, and titration on the recombinant adenovirus; taking dual-functional PEG as bridging agent; using amide reaction principle, and coupling PEG with Ad5-PTEN and EpDT3 to be EPAP successively, and confirming the coupling of PPEG-Ad5-PTEN by SDS-PAGE electrophoresis method; through fluorescence intensity detection method, grain diameter and Zeta potential detection, and TBE-PAGE electrophoresis method, identifying the formation of EPAP. The system and the method can efficiently target the EpCAM positive HepG2 hepatoma carcinoma cell and organizations, efficiently display the anti-cancer function, and the system becomes a new drug for the clinical gene treatment of liver cancer.

Description

technical field [0001] The invention belongs to the technical field of biotechnology drugs, and in particular relates to an aptamer-mediated adenovirus targeted drug delivery system and a construction method thereof. Background technique [0002] Traditional treatment methods for malignant tumors, such as surgery and chemotherapy, still cannot cure most cancers. The diagnosis of liver cancer is very difficult because of its high aggressiveness and migration. The surgical resection rate of liver cancer is usually less than 15%, but the postoperative recurrence rate is as high as 50%. In addition, although chemotherapy works on highly proliferative tumor cells and shrinks most tumors during the initial treatment phase, it does not prevent tumor recurrence and metastasis due to multidrug resistance. Furthermore, due to the lack of specific targeting agents for tumor stem cells, fatal toxicity and side effects caused by traditional chemotherapy are unavoidable. [0003] At pr...

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Application Information

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IPC IPC(8): C12N15/113C12N15/861A61K48/00A61P35/00
Inventor 钟志容刘中兵肖双礼王云龙易佑平孙小端邓若兰罗玉铃刘丽
Owner SOUTHWEST MEDICAL UNIVERISTY
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