Anti-African swine fever antibody and preparation method thereof

A technology of African swine fever and African swine fever virus, applied in the field of anti-African swine fever antibody and its preparation, can solve the problems of difficult implementation, hidden safety hazards, high cost, etc., and achieve the effect of mass production

Pending Publication Date: 2019-08-02
刘会芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the potential safety hazards in the current preparation of African swine fever antibodies, as well as the problems that the cost is too high and it is not easy to implement, the present invention provides an anti-African swine fever antibody

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment provides a method for preparing an anti-African swine fever antibody, which specifically includes the following steps:

[0025] 1. Poultry immunization: select 20-week-old healthy laying hens, and inject 0.5 mL of African swine fever subcutaneously through the breast muscle and neck subcutaneously for 3 times to eliminate the naturally attenuated strain of African swine fever (the poison price is 10 4 TCID 50 / mL), and immunized again every other week until the content of African swine fever antibody in yolk exceeded 40 ng / mL. Afterwards, a booster immunization was carried out every 15 days to maintain the anti-ASF antibody in the yolk > 40 ng / mL.

[0026] 2. Extraction and separation of egg yolk antibodies: collect the egg yolks that meet the antibody content conditions in step 1, add 3 times the volume of 2.5% w / w polyethylene glycol 6000 pure aqueous solution, stir and break the emulsion and let it stand for 2 hours to extract light yellow The super...

Embodiment 2

[0028] This embodiment provides a method for preparing an anti-African swine fever antibody, which specifically includes the following steps:

[0029] 1. Poultry immunization: select 20-week-old healthy laying hens, and inject 0.5 mL of African swine fever subcutaneously through the breast muscle and neck subcutaneously for 3 times to eliminate the naturally attenuated strain of African swine fever (the poison price is 10 5 TCID 50 / mL), and immunized again every other week until the content of African swine fever antibody in yolk exceeded 40 ng / mL. Afterwards, a booster immunization was carried out every 25 days to maintain the anti-ASF antibody in the yolk > 40 ng / mL.

[0030] 2. Extraction and separation of egg yolk antibodies: collect the egg yolks that meet the antibody content conditions in step 1, add 5 times the volume of 1.3% w / w polyoxyethylene polyoxypropylene pure aqueous solution, stir and break the emulsion, let it stand for 6 hours, extract the light The yello...

Embodiment 3

[0033] This embodiment provides a method for preparing an anti-African swine fever antibody, which specifically includes the following steps:

[0034] 1. Poultry immunization: select 20-week-old healthy laying hens, and inject 0.5 mL of African swine fever subcutaneously through the breast muscle and neck subcutaneously for 3 times to eliminate the naturally attenuated strain of African swine fever (the poison price is 10 6 TCID 50 / mL), and immunized again every other week until the content of African swine fever antibody in yolk exceeded 40 ng / mL. Afterwards, a booster immunization was carried out every 20 days to maintain the anti-ASF antibody in the yolk > 40 ng / mL.

[0035] 2. Extraction and separation of egg yolk antibodies: collect the egg yolks that meet the antibody content conditions in step 1, add 5 times the volume of 1.2% w / w polyoxyethylene polyoxypropylene pure aqueous solution, stir and break the emulsion, let it stand for 4 hours, extract the light The yello...

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Abstract

The invention relates to the technical field of livestock immunology and antibody preparation and especially relates to an anti-African swine fever antibody and a preparation method thereof. The antibody is prepared according to the following steps: using an African swine fever virus for immunizing a healthy female poultry animal; collecting yolks after the content of African swine fever antibodyin the yolks of the immunized poultry animal exceeds 40ng/mL; extracting water-soluble components in the yolks; purifying, thereby acquiring the antibody. The preparation technology for producing theantibody is simple, low-cost and high-feasibility and can be used for large-scale preparation of African swine fever antibody. The acquired antibody can be applied to contaminated pig farms and earlyinfected swine, so as to reduce the duplication and propagation of African swine fever virus.

Description

technical field [0001] The invention relates to the technical field of livestock and poultry immunology and antibody preparation, in particular to an anti-African swine fever antibody and a preparation method thereof. Background technique [0002] African swine fever (ASF for short) is a legally reported animal disease of the World Organization for Animal Health (OIE). It is caused by African swine fever virus infection. It is acute, febrile and highly contactable. sick. Since the first African swine fever occurred in Shenyang, Liaoning Province on August 3, 2018, it has spread to 28 provinces and cities in more than half a year, causing more than 100 epidemics and millions of pigs were culled. Since my country's pig farms (including sow farms, commodity pig farms, etc.) are mainly small and medium-sized pig farms with hundreds to thousands of heads, the number is large, the level of epidemic prevention and biosecurity is not high, and the distribution is very unreasonable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02
CPCC07K16/081C07K16/02
Inventor 刘会芳陈桂清宋勤叶王银钱高红梅袁洪兴李孝艳路强朱卫霞刘军同高云
Owner 刘会芳
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