Method for chemically inducing abalone allotriploid

A chemical induction and triploid technology, applied in animal husbandry and other fields, can solve the problems of undeveloped research, low induction rate, affecting embryonic development and larval survival, and achieve the effect of ensuring consistency

Active Publication Date: 2019-09-27
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no research has been carried out at home and abroad on the induction of allotriploids in wrinkled abalone (♀) and green abalone (♂).
At the same time, the allotriploid breeding of shellfish also has problems such as immature induction conditions, low induction rate, unstable induction effect, and induction factors affecting embryo development and larval survival. Solving the above factors will facilitate the promotion and application of this technology

Method used

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  • Method for chemically inducing abalone allotriploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Take wrinkled pan abalone and green abalone with good individual vitality and no external damage as seed abalone. The average shell length of wrinkled pan abalone (♀) is 76.09±9.57mm, the shell width is 50.66±4.19mm, and the wet weight is 55.50±13.88g. The average shell length of green abalone (♂) is 75.21±3.06mm, the shell width is 51.71±1.44mm, and the wet weight is 54.29±4.40g. The seed abalones are placed in different parent abalone breeding ponds for breeding, and the parent abalone breeding ponds in each parent abalone cultivation pond Bao's gender identity must be single.

[0030] 2) After the abalone gonads mature, dry them in the shade for 60 minutes, then place the male and female individuals in different clearly marked containers, inject ultraviolet-treated seawater with an irradiation intensity of 1000mWh / L, the seawater temperature is 22°C, and replace the ultraviolet treatment every 60min Seawater, until the gametes are discharged, the operation process...

Embodiment 2

[0038] 1) Take wrinkled pan abalone and green abalone with good individual vitality and no external damage as seed abalone. The average shell length of wrinkled pan abalone (♀) is 72.94±3.31mm, the shell width is 48.73±3.50mm, and the wet weight is 50.93±9.10g. The average shell length of green abalone (♂) is 75.93±0.98mm, the shell width is 51.31±1.36mm, and the wet weight is 52.22±3.50g. The seed abalones are placed in different parent abalone breeding ponds for breeding. Each parent abalone cultivation pond Qin Bao's gender characteristics must be single.

[0039] 2) After the abalone gonads mature, dry them in the shade for 60 minutes, then place the male and female individuals in different clearly marked containers, inject ultraviolet-treated seawater with an irradiation intensity of 900mWh / L, and the seawater temperature is 22°C, and replace the ultraviolet treatment every 60min Seawater, until the gametes are discharged, the operation process strictly prevents sperm and...

Embodiment 3

[0047] 1) Take wrinkled pan abalone and green abalone with good individual vitality and no external damage as seed abalone. The average shell length of wrinkled pan abalone (♀) is 76.61±5.06mm, the shell width is 51.95±2.88mm, and the wet weight is 54.43±7.57g. The average shell length of green abalone (♂) is 71.70±0.96mm, the shell width is 49.51±2.76mm, and the wet weight is 51.66±4.62g. The seed abalones are respectively placed in different parent abalone breeding ponds for breeding. Bao's gender identity must be single.

[0048] 2) After the abalone gonads mature, dry them in the shade for 60 minutes, then place the male and female individuals in different clearly marked containers, inject ultraviolet-treated seawater with an irradiation intensity of 1000mWh / L, and the seawater temperature is 21°C, and replace the ultraviolet treatment every 60min Seawater, until the gametes are discharged, the operation process strictly prevents sperm and eggs from contacting.

[0049] 3...

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Abstract

The invention discloses a method for chemically inducing an abalone allotriploid and relates to a method for cultivating shellfish. The method comprises the steps: (1) semen abalone selection and maturation promotion; (2) artificial spawning induction; (3) gamete selection and processing; (4) synchronous fertilization; (5) drug induction; (6) drug removal; (7) hatching. Synchronous fertilization is ensured: gametes are strictly prevented from being in contact in advance in the discharging process, it is confirmed through microscopic examination that accidental fertilization does not occur, and the consistency of synchronous fertilization and fertilized egg development is guaranteed. Induction conditions are optimized: 70%-80% of the PB1 proportion of fertilized eggs in a control group is determined as a processing time node by constant microscopic observation, it is ensured that the first polar body is effectively released, and a tetraploid and an aneuploidy are avoided. The stable and efficient induction rate is that the triploid induction rate is 100% or close to 100%.

Description

technical field [0001] The invention relates to a method for cultivating shellfish, in particular to a method for chemically inducing allotriploid abalone. Background technique [0002] Abalone is a rare marine treasure, known as the first of the "Eight Treasures of Seafood". As the world's largest abalone-raising country, my country's total abalone production in 2017 was 148,500 tons, with an annual output value of over 20 billion yuan. At present, the main species of abalone in my country is Haliotis discus hannai, and the aquaculture range extends from the Yellow Sea and the Bohai Sea in the north of my country to the coast of Fujian. The aquaculture output in Fujian Province reached 123,400 tons (2017), accounting for the total abalone in the country. 83.1% of the yield. However, due to the influence of the living habits of Abalone wrinkle, the abalone cultured in the subtropical Fujian sea area suffered from mass mortality many times in summer, and local fishermen suff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/0334
Inventor 王怡骆轩游伟伟柯才焕王星泽
Owner XIAMEN UNIV
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