A methylophilic bacterium capable of on-line monitoring of heavy metals and its application

A technology of methylophilic bacteria and heavy metals, applied in the field of microorganisms, can solve the problems that luminescent bacteria cannot be monitored online, and achieve the effects of low cost, fast response speed and simple operation

Active Publication Date: 2019-11-26
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, microorganisms, as indicator organisms, have many advantages such as low culture cost, short reproduction cycle, instant response and sensitivity. However, most of the microorganisms currently used for heavy metal toxicity monitoring are luminescent bacteria. Bacteria cannot be monitored online, freeze-dried powder needs to be prepared, and long-term strain recovery is required, which has certain limitations in environmental heavy metal and toxicity monitoring

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  • A methylophilic bacterium capable of on-line monitoring of heavy metals and its application
  • A methylophilic bacterium capable of on-line monitoring of heavy metals and its application
  • A methylophilic bacterium capable of on-line monitoring of heavy metals and its application

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Embodiment Construction

[0046] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0047] 1. Strain screening

[0048] On April 17, April 22, and April 27, 2015, we retrieved three sediment samples (numbered as sediment sample 1 and sediment sample 2 respectively) from the mouth of the wandering river in Yantai City, Shandong Province. , sediment sample 3), and then screened to get a strain of Pb 2+ and Hg 2+ All have strong respiratory inhibition response bacteria, the detailed process of screening is as follows:

[0049] (1) Strain enrichment

[0050] Take 1g of sediment sample 1 and place it in an Erlenmeyer flask containing 100mL of NCM liquid medium, culture it in a constant temperature incubator at 30°C with 140rpm shaking for 5 days, then add 1g of sediment sample 2 to it, and continue culturing for 5 days in the same way. Afterwards, 1 g of sediment sample 3 was added thereto, and the culture was continued f...

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Abstract

The invention discloses a methylophilic bacterium that can realize online monitoring of heavy metals and its application. The methylophilic bacterium is a strictly aerobic bacterium selected from the mouth of the Wandering River in Yantai, Shandong Province. Its aerobic respiration and oxygen consumption to Pb 2+ and Hg 2+ These two heavy metal ions respond sensitively, Pb 2+ and Hg 2+ It can strongly inhibit the respiration of the strain, and the preservation number of the strain is CGMCC No.13058. The benefits of the present invention are: the bacterial strain aerobic respiration and oxygen consumption have a significant effect on Pb 2+ and Hg 2+ The response of these two heavy metal ions is sensitive, and by monitoring the change of oxygen consumption rate, the concentration of Pb in drinking water, fresh surface water and seawater can be realized. 2+ , Hg 2+ Rapid response and early warning of heavy metal ions, fast response and simple operation; (2) The strain can be automatically cultivated without the need for a large amount of freeze-dried powder of bacteria, and the cost is lower; (3) The strain can be used in real-time heavy metal monitoring and online The research and development of early warning technology and instruments have great application prospects.

Description

technical field [0001] The invention relates to a methylophilic bacterium and its application, in particular to a methylophilic bacterium capable of realizing heavy metal on-line monitoring and its application, belonging to the technical field of microorganisms. Background technique [0002] In more than a century after the second industrial revolution, human industrial and agricultural production capacity has increased rapidly, and human living standards have been greatly improved. At the same time, there are serious environmental pollution problems. Among them, heavy metal pollution It has always been the focus of pollutants in water pollution. [0003] For nearly a century, human heavy metal poisoning incidents caused by eating fish, shrimp, crab shells enriched in heavy metals have been common. The earliest report was a cadmium poisoning incident (bone pain disease) caused by mine wastewater pollution in Japan in the 1930s . Similar incidents followed. In the 1950s, so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/02G01N33/18C12R1/01
CPCC12Q1/02C12N1/205C12R2001/01G01N33/1866Y02A20/20
Inventor 陈令新王巧宁付龙文孙西艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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