Specific amplification primer for microsatellite locus M009 of sika deer and application of specific amplification primer

Abstract

The invention discloses a specific amplification primer for microsatellite locus M900 of sika deer and an application of the specific amplification primer. The nucleotide sequence of the specific amplification primer for microsatellite locus M009 is shown in SEQ. ID. NO: 1 and SEQ. ID. NO: 2. The microsatellite locus M009 for the sika deer is applied to the analysis of antler growth traits in thesika deer. The growth traits of the antler are selected according to the genotype, and individuals with high yield traits and low yield traits of the antler can be screened, high accuracy, simple operation and low cost are achieved, the growth traits of the antler can be selected early in the life of the sika deer, the generation interval is shortened, the selection intensity is improved, and thebreeding process of the sika deer is sped up; the specific amplification primer for the microsatellite locus M900 of the sika deer and the application of the specific amplification primer are applicable to the field of breeding of the sika deer.

Description

technical field [0001] The invention relates to a specific amplification primer for sika deer microsatellite site M009 and application thereof. Background technique [0002] Sika deer antler, as a traditional and precious medical and health care medicinal material, has high economic value. At present, the key issue affecting the economic benefits of my country's sika deer breeding industry is to increase the production of antler. However, the high-yielding trait of velvet antler is controlled by multiple genes, and it is a quantitative trait with low heritability. Using traditional breeding techniques, the cycle is relatively long. For example, the breeding of Shuangyang sika deer takes up to 50 years, and it is difficult to carry out early selection. Therefore, at present, many scholars use molecular genetic markers to study the production traits of sika deer antler from the molecular level. Molecular genetic markers can effectively overcome the shortcomings of traditiona...

AI Technical Summary

Problems solved by technology

Due to the above reasons, only a limited number of SSR molecular markers related to the growth traits of sika deer antler have been screened, which greatly limits the construction of the physical map of sika deer and further in-depth research on molecular breeding of sika deer for antler.

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