Human mantle cell lymphoma malignant clone cell strain and construction method and application thereof

A technology for mantle cell lymphoma and its establishment method, which is applied to the field of malignant clonal cell lines of tumors and their establishment, and can solve the problems of difficulty in obtaining, difficulty in culturing, and poor cell activity, etc.

Active Publication Date: 2019-11-12
重庆医药高等专科学校附属第一医院
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Problems solved by technology

The biggest problem in the above research is to establish a single cell component cell line as pure as possible in the early stage, while ignoring the fact that the early malignant clone tumor cells with stemness are also undergoing continuous differentiation, and the malignant clones of each stage of the tumor are mutually differentiated Treated in isolation, this kind of cell model research is so

Method used

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  • Human mantle cell lymphoma malignant clone cell strain and construction method and application thereof
  • Human mantle cell lymphoma malignant clone cell strain and construction method and application thereof
  • Human mantle cell lymphoma malignant clone cell strain and construction method and application thereof

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Embodiment

[0040] 1. Establishment of human mantle cell lymphoma malignant clonal cell line JeKo-1-spheroid

[0041] A human mantle cell lymphoma malignant clone cell line containing early malignant clones, named JeKo-1-spheroid, is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is C2018264.

[0042] The JeKo-1-parenta in the embodiment of the present invention was purchased from the Cell Bank of the Chinese Academy of Sciences.

[0043] The establishment method of the human mantle cell lymphoma malignant clone cell line JeKo-1-spheroid is: based on the human mantle cell lymphoma cell line JeKo-1-parenta, the human mantle cell lymphoma malignant clone is established by continuous culture and natural purification method Cell line JeKo-1-spheroid, the so-called continuous culture natural purification method is:

[0044] 1) After recovering the human mantle cell lymphoma cell line JeKo-1-parental, use RPMI-1640 medium containing 20% ​​fetal b...

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Abstract

The invention belongs to the technical field of construction of tumor cell lines, and particularly relates to a tumor malignant clone cell line having important abnormal development disintegrating stage of mantle cell lymphoma, and a construction method and application of the tumor malignant clone cell line. The invention provides a human mantle cell lymphoma malignant clone cell strain containingearly malignant clone cells, is named as a human mantle cell lymphoma malignant clone cell strain JeKo-1-spheroid, and is preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is C2018264. A mature JeKo-1-parental cell strain is used as an induction object, a continuous culture and natural purification methods are used, a malignant clone cell strain containinga mantle cell lymphoma main malignant disintegrating stage is obtained, and the obtained cell strain highly cooperates with primary cells of a patient.

Description

technical field [0001] The invention belongs to the technical field of establishment of tumor cell lines, and in particular relates to a tumor malignant clone cell line with an important abnormal development and differentiation stage of mantle cell lymphoma and an establishment method thereof. Background technique [0002] Tumors are composed of cell groups with malignant clonal proliferation at different stages. Among them, the evolution of malignant clones is the most important to the occurrence and evolution of tumors, especially the early stage clones, which is of great significance for us to further comprehensively and objectively study tumors. The hotspot of tumor research focuses on the evolution of malignant clones between cells in the abnormal stage of differentiation and development, which not only further deepens the understanding of tumor occurrence, but also has a profound impact on tumor diagnosis and treatment, prognosis judgment, and drug development. [0003...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12R1/91
CPCC12N5/0693
Inventor 唐君玲张力李晓明邢宏运周铁军周仕霞王季马涛韩丽英周玉萍王建英王雪梅吴洪燕谭红梅胡敏汤计瑞郭静丁莉刘丹杜鹃
Owner 重庆医药高等专科学校附属第一医院
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