Method of using triptonide for alleviating mouse hyperalgesia reaction induced by CFA

A technology of triptolide ketone and mice, applied in the field of triptolide ketone to reduce CFA-induced mouse hyperalgesia

Pending Publication Date: 2020-01-10
NANTONG UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

[0003] Since the hyperalgesic response can provide direct evidence for the anti-nociceptive and anti-inflammatory effects, and can suggest the role of TPN in the treatment of inflammatory pain, but there is no relevant experimental method for TPN in the treatment of inflammatory pain. Therefore, there is an urgent need for a Improved technology to solve this problem existing in the prior art

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  • Method of using triptonide for alleviating mouse hyperalgesia reaction induced by CFA
  • Method of using triptonide for alleviating mouse hyperalgesia reaction induced by CFA
  • Method of using triptonide for alleviating mouse hyperalgesia reaction induced by CFA

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Embodiment Construction

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] The present invention provides a technical scheme: a method for triptolide ketone to alleviate CFA-induced hyperalgesia in mice, comprising the following steps:

[0022] Step 1: Unilateral injection of complete Freund's adjuvant (CFA) into the mouse hind paw can cause paw swelling and persistent pain hypersensitivity;

[0023] Step 2: Inject TPN intravenously after CFA injection, and detect changes in paw swelling, mechanical hyperalgesia, and thermal h...

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Abstract

The invention discloses a method of using triptonide for alleviating mouse hyperalgesia reaction induced by a CFA. The method includes the steps: 1, the CFA is injected into a unilateral rear foot ofa mouse; 2, after CFA injection, the TPN is subjected to intravenous injection; 3, expression changes of AKT of neurons in dorsal root ganglia (DRG) after the CFA is injected are detected; 4, expression changes of mRNA of a tumor necrosis factor alpha, interleukin 1[beta] and interleukin 6 of the DRG neurons after the CFA is injected are detected; and 5, an AKT inhibitor is used for preprocessing,changes of mechanical hyperalgesia and thermal hyperalgesia which are caused by the CFA are detected, and the expression changes of mRNA of proinflammatory cytokines such as the TNF-alpha, the IL-1[beta] and the IL-6 are detected. Through the method of using the triptonide for alleviating the mouse hyperalgesia reaction induced by the CFA, results can be obtained, direct evidence is provided foranti-nociceptive and anti-inflammatory effects of the TPN, and new application of the TPN in inflammatory pain treatment is indicated.

Description

technical field [0001] The invention relates to the technical field of hyperalgesia, in particular to a method for triptolide ketone to reduce the hyperalgesia induced by CFA in mice. Background technique [0002] Triptolide ketone (TPN) is the main component of Tripterygium wilfordii. Recent studies have shown that the PI3K / AKT / mTOR pathway plays an important role in the pathogenesis of chronic pain. Complete Freund's adjuvant (CFA) is a commonly used inflammatory agent and is currently the most commonly used adjuvant in animal experiments. [0003] Since the hyperalgesic response can provide direct evidence for the anti-nociceptive and anti-inflammatory effects, and can suggest the role of TPN in the treatment of inflammatory pain, but there is no relevant experimental method for TPN in the treatment of inflammatory pain. Therefore, there is an urgent need for a Improved technology solves this problem existing in the prior art. Contents of the invention [0004] The pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61P29/00A61K49/00
CPCA61K31/585A61P29/00A61K49/0008
Inventor 董玉林姜保春
Owner NANTONG UNIVERSITY
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