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Construction method of mouse model of extramammary Paget disease

A mouse model and construction method technology, which can be used in chemical instruments and methods, biochemical equipment and methods, and microbial determination/examination.

Active Publication Date: 2021-02-02
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Regarding Paget-like disease, there is no good treatment at present. Surgery is the first choice for the treatment of breast-like Paget-like disease. The main reason is that the pathogenesis of Paget-like disease is still not very clear, which affects the development of drug targets. Therefore, it is possible to establish The mouse model of mammary gland-like paget-like disease plays a very important role in the study of the pathogenesis of mammary gland-like paget-like disease, and is a milestone for further research on the drug treatment of the disease

Method used

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  • Construction method of mouse model of extramammary Paget disease
  • Construction method of mouse model of extramammary Paget disease
  • Construction method of mouse model of extramammary Paget disease

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Embodiment 1

[0039] The construction method of the paget disease mouse model (DTG mouse) outside the embodiment 1 mammary gland

[0040] (1) Preparation of extramammary Paget disease mouse model (inducible mouse model)

[0041] The K14rtta transgenic mice were mated with the TRE-Msi1 transgenic mice to produce offspring mice. When the offspring mice grew to 6 weeks, tetracycline with a final concentration of 0.2 g / L was added to the drinking water of the mice to allow The mice drank water freely, ensured sufficient drinking water, and overexpressed the induced gene Musashi1. After feeding tetracycline for 48 hours, mice with extramammary Paget disease-like symptoms can be seen, that is, extramammary Paget disease mice (DTG mice).

[0042] (2) Identification of DTG mouse genotype

[0043] 1. Extract mouse genomic DNA as a template, use F1 and R1 as primers, perform PCR amplification, detect the amplified product, and a characteristic band of 500bp appears. PCR reaction system: mix 6 μL (...

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Abstract

The invention provides a method for constructing a mouse model of extramammary paget disease. K14rtta transgenic mice are mated with TRE-Msi1 transgenic mice, and the offspring mice produced are fed tetracycline, and a small gene Musashi1 overexpressed is detected. Mice are extramammary Paget disease mice. The extramammary paget disease mouse model constructed by the invention lays a theoretical foundation for systematic research on the disease from the molecular mechanism in the future.

Description

technical field [0001] The invention relates to a method for constructing a disease animal model, in particular to a method for constructing an extramammary paget disease mouse model. Background technique [0002] Paget's disease of the breast was discovered by James Paget in 1874 (Paget J, 1874, St Barth Hosp Rep 1874; 10:87-9). The etiology is still unclear, and it can be divided into breast Paget disease and extramammary Paget disease. Clinical features of breast Paget disease: eczema-like changes in the areola, mostly unilateral, manifested as itchy nipples, red skin on the nipple, and extramammary Paget disease Frequently occurs in the armpits, scrotum, around the anus and other parts. Breast Paget's disease and extramammary Paget's disease have similar clinical features, with a large number of paget-like cells in the epidermis (GUOHAI SHUI, 2010, APMIS118:777-781). Express some common markers. Such as breast paget: AR (88%), ER (10%), PR (0%). Extramammary paget: A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02C12Q1/6888
CPCA01K67/027C07K14/47C12Q1/6888
Inventor 于政权宋永利
Owner CHINA AGRI UNIV
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