Culture method and medium for non-transgenic papaya seedling culture
A non-transgenic, cultivation method technology, applied in the field of non-transgenic papaya seedling cultivation method and its culture medium, can solve the problems of long time period for seedling cultivation, production waste, and inability to guarantee the seedling rate of seeds, etc., to achieve rich nutrition, Good storage resistance and good elasticity
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Embodiment 1
[0027] 1. Disinfection of explants: Select clean and virus-free long-term lateral buds, remove leaves, and rinse with running water for half an hour. Then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% raw mercury for 12 minutes, and then wash with sterile water 5 times, 2 minutes each time. Finally, it was placed in a sterile bottle, inoculated in the prepared MS medium and cultured for 30 days.
[0028] 2. Proliferation cultivation of secondary buds: cutting the primary buds on the explants and placing them on the proliferation medium of secondary buds for 30 days, after 30 days, the multiplication factor of secondary buds was 2.98, and the growth of buds was good. The optimal proliferation medium formula is 3 / 2MS+BA0.2mg / L+GA0.5mg / L.
[0029] 3. Induction of rooting: Place the well-growing secondary shoots in the rooting medium and cultivate them for 30 days. After 30 days, the secondary shoots have good rooting and the roots ar...
Embodiment 2
[0032] 1. Disinfection of explants: Select clean and virus-free long-term lateral buds, remove leaves, and rinse with running water for half an hour. Then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% raw mercury for 12 minutes, and then wash with sterile water 5 times, 2 minutes each time. Finally, it was placed in a sterile bottle, inoculated in the prepared MS medium and cultured for 28 days.
[0033] 2. Proliferation cultivation of secondary buds: cutting the primary buds on the explants and placing them on the proliferation medium of secondary buds for 25 days, after 25 days, the multiplication factor of secondary buds was 2.77, and the growth of buds was good. Proliferation medium formula is 1.2MS+BA0.15mg / L+GA 0.45mg / L.
[0034] 3. Induction of rooting: Place the well-growing secondary shoots in the rooting medium and cultivate them for 30 days. After 30 days, the secondary shoots have good rooting and the roots are relativ...
Embodiment 3
[0037] 1. Disinfection of explants: Select clean and virus-free long-term lateral buds, remove leaves, and rinse with running water for half an hour. Then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% raw mercury for 12 minutes, and then wash with sterile water 5 times, 2 minutes each time. Finally, it was placed in a sterile bottle, inoculated in the prepared MS medium and cultured for 33 days.
[0038] 2. Proliferation and cultivation of secondary buds: cutting primary buds on the explants was placed on the proliferation medium of secondary buds and cultivated for 30 days. After 30 days, the multiplication factor of secondary buds was 2.91, and the growth of buds was good. Proliferation medium formula is 1.6 MS+BA0.24mg / L+GA 0.6mg / L.
[0039] 3. Induction of rooting: Place the well-growing secondary shoots in the rooting medium and cultivate them for 30 days. After 30 days, the secondary shoots have good rooting and the roots are ...
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