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Culture method and medium for non-transgenic papaya seedling culture

A non-transgenic, cultivation method technology, applied in the field of non-transgenic papaya seedling cultivation method and its culture medium, can solve the problems of long time period for seedling cultivation, production waste, and inability to guarantee the seedling rate of seeds, etc., to achieve rich nutrition, Good storage resistance and good elasticity

Inactive Publication Date: 2020-03-27
HAINAN TIANYULIANGPIN AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the characteristics of the seeds themselves, seeds in the dormant period need to break the dormancy to successfully grow seedlings, but this method takes a long time to grow seedlings, and at the same time cannot guarantee a high seedling rate
[0003] In addition, papaya is dioecious, and has three types of plants: female plant, male plant and hermaphrodite plant, which need to be distinguished by flowering and fruiting, which will cause a lot of waste in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Disinfection of explants: Select clean and virus-free long-term lateral buds, remove leaves, and rinse with running water for half an hour. Then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% raw mercury for 12 minutes, and then wash with sterile water 5 times, 2 minutes each time. Finally, it was placed in a sterile bottle, inoculated in the prepared MS medium and cultured for 30 days.

[0028] 2. Proliferation cultivation of secondary buds: cutting the primary buds on the explants and placing them on the proliferation medium of secondary buds for 30 days, after 30 days, the multiplication factor of secondary buds was 2.98, and the growth of buds was good. The optimal proliferation medium formula is 3 / 2MS+BA0.2mg / L+GA0.5mg / L.

[0029] 3. Induction of rooting: Place the well-growing secondary shoots in the rooting medium and cultivate them for 30 days. After 30 days, the secondary shoots have good rooting and the roots ar...

Embodiment 2

[0032] 1. Disinfection of explants: Select clean and virus-free long-term lateral buds, remove leaves, and rinse with running water for half an hour. Then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% raw mercury for 12 minutes, and then wash with sterile water 5 times, 2 minutes each time. Finally, it was placed in a sterile bottle, inoculated in the prepared MS medium and cultured for 28 days.

[0033] 2. Proliferation cultivation of secondary buds: cutting the primary buds on the explants and placing them on the proliferation medium of secondary buds for 25 days, after 25 days, the multiplication factor of secondary buds was 2.77, and the growth of buds was good. Proliferation medium formula is 1.2MS+BA0.15mg / L+GA 0.45mg / L.

[0034] 3. Induction of rooting: Place the well-growing secondary shoots in the rooting medium and cultivate them for 30 days. After 30 days, the secondary shoots have good rooting and the roots are relativ...

Embodiment 3

[0037] 1. Disinfection of explants: Select clean and virus-free long-term lateral buds, remove leaves, and rinse with running water for half an hour. Then disinfect with 75% alcohol for 30 seconds on the ultra-clean workbench, disinfect with 0.1% raw mercury for 12 minutes, and then wash with sterile water 5 times, 2 minutes each time. Finally, it was placed in a sterile bottle, inoculated in the prepared MS medium and cultured for 33 days.

[0038] 2. Proliferation and cultivation of secondary buds: cutting primary buds on the explants was placed on the proliferation medium of secondary buds and cultivated for 30 days. After 30 days, the multiplication factor of secondary buds was 2.91, and the growth of buds was good. Proliferation medium formula is 1.6 MS+BA0.24mg / L+GA 0.6mg / L.

[0039] 3. Induction of rooting: Place the well-growing secondary shoots in the rooting medium and cultivate them for 30 days. After 30 days, the secondary shoots have good rooting and the roots are ...

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Abstract

The invention relates to a culture method and medium for non-transgenic papaya seedling culture. The culture medium comprises n* MS and the following components in mass concentration: 0-30 g / L of sugar, 0-0.8 mg / L of GA, 0-0.3 mg / L of 6-BA and 0-0.3 mg / L of IBA, wherein n represents the product of the content of major elements in the MS culture medium and n times; and the major elements refer to KNO3, NH4NO3, MgSO4, KH2PO4 and CaCl2. The culture medium for non-transgenic papaya seedling culture provided by the invention contains various nutritional ingredients required by growth of papaya seedlings, and the papaya seedlings are high in absorption speed and high in survival rate. According to the culture method for non-transgenic papaya seedling culture provided by the invention, the cultured papaya is fragrant and sweet in taste, the fructose degree reaches 16-18%, and the papaya is glossy in appearance, rich in nutrition, high in elasticity, long in storage time and high in yield.

Description

technical field [0001] The invention relates to the technical field of non-transgenic agricultural cultivation, in particular to a method for cultivating non-transgenic papaya seedlings and a culture medium thereof. Background technique [0002] Traditional non-GMO papayas are raised by sowing seeds. Due to the characteristics of the seeds themselves, the seeds in the dormant period need to break the dormancy to successfully grow seedlings, but the time period for seedling cultivation in this method is very long, and it cannot guarantee that the seeds have a high seedling rate. [0003] In addition, pawpaw has dioecious plants, which have three types of plants: female plant, male plant and hermaphrodite plant, which need to be distinguished by flowering and fruiting, which will cause a lot of waste in production. [0004] Therefore, need to provide a kind of culture method of new non-transgenic papaya seedling cultivation. Contents of the invention [0005] In order to s...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G24/25
CPCA01H4/001A01H4/005A01H4/008A01G24/25
Inventor 侯源耀梁可平程金坚
Owner HAINAN TIANYULIANGPIN AGRI DEV CO LTD
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