A construction method and identification method of uplc characteristic map of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules

A technology of formula granules and characteristic map, applied in the directions of instruments, measuring devices, scientific instruments, etc., can solve the problem that the qualitative and quantitative control of bran fried Atractylodis japonica is limited to, and has not yet been controlled by the characteristic map, and achieves good peak separation. , the method is stable, the effect of high precision

Active Publication Date: 2021-09-28
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the research on the quality standards of bran-fried beijingzhu formula granules and standard decoction, and the 2015 edition of "Chinese Pharmacopoeia" controls the qualitative and quantitative control of bran-fried beijingzhu only for its atractylodes components, and has not yet been carried out. Its characteristic map control

Method used

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  • A construction method and identification method of uplc characteristic map of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules
  • A construction method and identification method of uplc characteristic map of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules
  • A construction method and identification method of uplc characteristic map of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules

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Effect test

Embodiment 1

[0044] Construction of UPLC characteristic maps of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules.

[0045] 1. Test method

[0046] 1.1 Preparation of reference substance solution: (1) Take atractylodes and chlorogenic acid reference substances, weigh them accurately, add methanol to make a mixed solution containing 20μg per 1ml, shake well, and obtain. (2) Take 0.4g of Atractylodes atractylodis as reference drug, accurately weigh it, put it into a stoppered Erlenmeyer flask, accurately add 15ml of 50% methanol, weigh it, treat it with ultrasound for 30 minutes, let it cool, and make up for the lost weight with 50% methanol, Shake well, filter, and take the continued filtrate.

[0047] 1.2 Chromatographic conditions: use octadecylsilane bonded silica gel as filler, acetonitrile as mobile phase A, 0.1% formic acid as mobile phase B, carry out gradient elution according to the provisions in Table 6; the flow rate is 0.3ml per minu...

Embodiment 2

[0072] A method for identifying bran-fried Atractylodes herb decoction pieces, standard decoction and formula granules, comprising the following steps:

[0073] (1) Precisely weigh the decoction pieces of bran-fried Atractylodes atractylodes to be identified, standard decoction and formula granules, and prepare sample solutions of decoction pieces of bran-fried Atractylodes atractylodis to be identified, standard decoction and formula granules;

[0074] (2) Precisely absorb the sample solution of bran-fried Atractylodes decoction pieces to be identified, standard decoction and formula granules, inject them into an ultra-high performance liquid chromatograph for measurement, and obtain the UPLC characteristics of the unidentified bran-fried Atractylodes decoction pieces, standard decoction and formula granules Atlas;

[0075] (3) Compare the measured UPLC characteristic atlas with the constructed UPLC characteristic atlas of bran-fried Atractylodes herb decoction pieces, standa...

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Abstract

The invention relates to a method for constructing UPLC characteristic maps of Bran-fried Atractylodes Rhizome, standard decoction and formula granules and its identification method, comprising the following steps: (1) Accurately weighing Bran-fried Atractylodes Rhizome decoction pieces, standard decoction and formula granule powder , prepared the test solution of bran-fried Atractylodes herb decoction pieces, standard decoction and formula granules; (2) analyzed the test solution of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules by ultra-high performance liquid chromatography, and obtained Bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules UPLC characteristic chromatograms. The present invention adopts and establishes the UPLC characteristic spectrum of bran-fried Atractylodes herb decoction pieces, standard decoction and formula granules, and determines 9 common peaks, of which No. 9 peak is Atractylodes atractylodes peak; The UPLC characteristic chromatograms of decoction and formula granules provide a rapid and comprehensive detection method for the quality of bran-fried Atractylodes atractylodes decoction pieces, standard decoction and formula granules.

Description

technical field [0001] The invention belongs to the field of identification of traditional Chinese medicines, and in particular relates to a construction method and an identification method of UPLC characteristic maps of bran-fried Atractylodes atractylodis, standard decoction and formula granules. Background technique [0002] Bran-fried Atractylodes atractylodes is a processed product of the dried rhizome of Atractylodes atractylodes, a plant of the Compositae family. (Recorded in the 2015 edition of "Chinese Pharmacopoeia"), which is a commonly used traditional Chinese medicine. It has the functions of drying dampness and invigorating the spleen, expelling wind and cold, and treating dampness; it is used for damp resistance in the middle of the burner, abdominal fullness, diarrhea, edema, beriberi, flaccidity, wind-cold cold, night blindness, and dim eyesight. Its nature and flavor are pungent, bitter and warm. Return spleen, stomach, liver channel. [0003] The main c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/86
CPCG01N30/06G01N30/34G01N30/8679G01N30/8686G01N30/88
Inventor 何广铭罗宇琴杨文惠魏梅孙冬梅陈向东马瑞瑞罗思妮程学仁
Owner GUANGDONG YIFANG PHARMA
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