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Method for preparing stem-cell active-factor composition with muscle cell repairing function

A technology of muscle cells and active factors, used in health care products and medical fields, to achieve the effect of obvious cell aging, obvious muscle repair, and improvement of cell aging

Inactive Publication Date: 2020-05-29
山东卓东生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with many adult stem cells, the research and clinical application of muscle stem cells in China is still lagging behind.

Method used

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  • Method for preparing stem-cell active-factor composition with muscle cell repairing function
  • Method for preparing stem-cell active-factor composition with muscle cell repairing function
  • Method for preparing stem-cell active-factor composition with muscle cell repairing function

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Experimental program
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Embodiment

[0030] A preparation method of a stem cell active factor composition with muscle cell repair function, comprising the following steps:

[0031] 1. the method for separating and culturing muscle stem cells, comprising the following steps:

[0032] 1.1 Take 5g of deltoid muscle from a young individual or patient. The required muscle tissue can be not limited to normal muscle, but can also be traumatized pathological muscle. Traumatized muscle usually contains stem cells in a more activated state within 3-7 days of trauma , with faster growth rate and stronger regeneration ability;

[0033] 1.2 Wash the muscle obtained in step 1.1 with sterile, pyrogen-free phosphate buffered saline buffer (PBS);

[0034] 1.3 The muscle tissue obtained in step 1.2 is successively minced until the particle size of the tissue is not greater than 1mm, and the muscle tissue homogenate is obtained;

[0035] 1.4 Homogenate the muscle tissue obtained in step 1.3 according to the enzyme substrate conce...

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Abstract

The invention relates to the technical field of medical and health-care products and particularly relates to a method for preparing a stem-cell active-factor composition with a muscle cell repairing function. The method comprises the steps: firstly, acquiring autogenous or xenogenous human muscle stem cells with active regeneration capacity, and carrying out mass amplification through in-vitro culture, so as to establish a cell line for long-term application; and breaking the cells with ultrasonic waves under low-temperature conditions, carrying out centrifugation, then, collecting supernatant, carrying out filtering and concentrating so as to obtain a muscle stem cell extract, merging an extract of a stem cell culture solution, adding an activity stabilizer and a preservative, and then, carrying out mixing with a penetrant, thereby obtaining the active-factor composition. The method has the beneficial effects that the stem-cell active-factor composition can exert very good repairing and regrowing actions on impaired muscle and skin; and an improvement on a senescence state of the skin can be promoted.

Description

technical field [0001] The invention relates to the technical field of medical and health care products, in particular to a preparation method of a stem cell active factor composition with muscle cell repair function. Background technique [0002] Stem cells have extremely broad application prospects in the fields of cell therapy, tissue and organ repair, developmental biology, and pharmacology. It plays an increasingly important role in basic research and clinical application. However, compared with many adult stem cells, the research and clinical application of muscle stem cells in China is still lagging behind. Skeletal muscle is one of the most important muscles in the body, responsible for supporting a variety of bodily functions. Unlike all other tissues, skeletal muscle and muscular tissue are specialized in controlling active movement, while individuals' strenuous activities, sports exercises, and even daily actions are constantly creating physical micro-damages to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/34A61P21/00A61P39/06
CPCA61K35/34A61P21/00A61P39/06
Inventor 牟晓东
Owner 山东卓东生物科技有限公司
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