Extension knee apparatus allogeneic transplantation animal model and establishment method thereof

An allogeneic and animal model technology, applied in the field of medical transplantation, can solve problems such as the lack of animal models of knee extension devices

Pending Publication Date: 2020-07-28
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Animal models are the most effective way to study whether the treatment of human diseases is feasible in vivo, but there are no related reports on animal models of knee extensor device allografts, so to establish an animal mo

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  • Extension knee apparatus allogeneic transplantation animal model and establishment method thereof
  • Extension knee apparatus allogeneic transplantation animal model and establishment method thereof
  • Extension knee apparatus allogeneic transplantation animal model and establishment method thereof

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Embodiment Construction

[0032] The technical solution of the present invention will be described in more detail and complete below in conjunction with the accompanying drawings.

[0033] 1. Knee extensor device preparation before allografting

[0034] 1. New Zealand white rabbits were used as the test object, and the right lower limb was used as the operation side. 2% pentobarbital sodium 40mg / kg intraperitoneal injection, after anesthesia, fix the limbs in the supine position, wear sterile gloves, surgical cap, mask, 8% sodium sulfide after depilation of the lower limbs, wipe with clean water, disinfect with aner iodine, and lay the sheets , change sterile gloves.

[0035] 2. Take the median incision of the knee joint, cut the skin, subcutaneous tissue, and fascia layer by layer, and expose the knee extension device ( figure 1 ), make a 0.5cm long longitudinal incision on the outside of the joint capsule, through which the vascular forceps penetrate the joint capsule to the inside, make an incisio...

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Abstract

The invention discloses an extension knee apparatus allogeneic transplantation animal model and an establishment method thereof. A New Zealand white rabbit is used as a test object, and the establishment method of the model comprises the following steps: S1, preparation of a graft: taking down a whole extension knee apparatus under a sterile condition, and performing sterile and ultralow-temperature cryopreservation; s2, allogeneic transplantation of the extension knee apparatus: (1) anesthesia, unhairing, disinfection and sheet laying of an allogeneic transplantation object; (2) cutting the middle of the knee joint, longitudinally cutting the whole extension knee apparatus, removing patella and tibial tubercle to form bone grooves, and penetrating a steel wire into the bottom; (3) unfreezing a graft, suturing a quadriceps femoris tendon by using a Krackow method, binding and fixing the tibia in a tibia groove by using a steel wire, and knotting after a suture of the quadriceps femoristendon penetrates out of the quadriceps femoris muscle; and (4) sequentially suturing, straightening and fixing with gypsum. According to the animal model, animal experiments prove that allogeneic transplantation of the extension knee apparatus is a feasible treatment method for incomplete functions of the extension knee apparatus for the first time, and an important experimental basis is provided for clinical development of allogenic transplantation of the human extension knee apparatus.

Description

technical field [0001] The invention relates to the technical field of medical transplantation, in particular to a knee extension device allograft animal model and a method for establishing the same. Background technique [0002] The knee extensor apparatus is a complex tissue that performs the function of knee extension and consists of the quadriceps tendon, patella, patellar ligament, and tibial tuberosity. Extensor mechanism insufficiency includes rupture of the quadriceps tendon and patellar ligament and fractures of the patella and tibial tuberosity, with an overall incidence of approximately 0.5% to 6%. The treatment of knee extensor insufficiency is relatively difficult, mainly including direct repair and surrounding tissue reinforcement repair, and severe knee extensor insufficiency, especially the knee extensor insufficiency that occurs after total knee replacement, once it occurs, it is a disaster sexual. [0003] Knee extensor device allografts are composite tis...

Claims

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Application Information

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IPC IPC(8): A61D1/00A01K67/02
CPCA01K67/02A61D1/00
Inventor 陈观印马保安
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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