A kind of probiotic and its preparation method and application

A technology of micro-ecological preparations and diluents, applied in the field of poultry farming, can solve the problems of not being able to improve the quality of eggs

Active Publication Date: 2022-07-26
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, a kind of microecological preparation that can replace antibiotics and realize chicken antibiotic-free breeding meets contemporary needs, but the microecological preparations in the prior art, such as CN201510856151. It is mainly aimed at the production performance of chickens and cannot improve the quality of eggs

Method used

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  • A kind of probiotic and its preparation method and application
  • A kind of probiotic and its preparation method and application
  • A kind of probiotic and its preparation method and application

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preparation example Construction

[0039]The present invention provides a method for preparing the microecological preparation according to the above technical solution, which includes the following steps: inoculating the Bacillus licheniformis BDY11 in a LB liquid medium, culturing in a liquid state to obtain a Bacillus licheniformis BDY11 liquid fermentation liquid, and inoculating the Bacillus licheniformis BDY11 Bacillus subtilis BDY11 liquid fermentation broth is centrifuged to obtain Bacillus licheniformis BDY11 cells; the Bacillus subtilis BKC23 strain is inoculated in LB liquid medium, and the Bacillus subtilis BKC23 liquid fermentation broth is obtained by liquid culture, and the Bacillus subtilis BKC23 liquid fermentation liquid is fermented The liquid is centrifuged to obtain Bacillus subtilis BKC23 cells; the Bacillus licheniformis BDY11 cells are mixed with Bacillus subtilis BKC23 cells to obtain mixed cells; the mixed cells are mixed with an adsorption diluent and dried to obtain a microecological p...

Embodiment 1

[0049] Compound probiotic: the content of Bacillus licheniformis BDY11 is 5×10 9 CFU / g, Bacillus subtilis BKC23 content was 2×10 10 CFU / g.

[0050] Inoculate Bacillus licheniformis BDY11 on LB plate and activate and culture at 37°C for 12 hours to obtain a single colony of Bacillus licheniformis BDY11; inoculate a single colony of Bacillus licheniformis BDY11 on LB liquid medium, at 37°C temperature and rotating speed of 200r Under the condition of / min, the liquid culture was carried out for 24h, and the concentration was 5×10 9 CFU / ml Bacillus licheniformis BDY11 liquid fermentation broth, centrifuging the Bacillus licheniformis BDY11 liquid fermentation broth to obtain Bacillus licheniformis BDY11 cells;

[0051] Inoculate Bacillus subtilis BKC23 on LB plate at a temperature of 37°C for activation and culture for 12 hours to obtain a single colony of Bacillus subtilis BKC23; inoculate a single colony of Bacillus subtilis BKC23 on LB liquid medium at 37°C with a rotating s...

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Abstract

The invention belongs to the technical field of poultry breeding, and in particular relates to a microecological preparation and a preparation method and application thereof. The invention provides a microecological preparation, comprising Bacillus subtilis BKC23 and Bacillus licheniformis BDY11; the preservation number of the Bacillus subtilis BKC23 is CCTCC NO: M2019907; the preservation number of the Bacillus licheniformis BDY11 is CCTCC NO: M2019908 . The microecological preparation provided by the invention can not only improve the production performance of chickens and the quality of eggs, but also have a positive impact on the balance of fecal microorganisms in laying hens, reduce the number of pathogenic bacteria and increase the number of beneficial fecal bacteria.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding, and in particular relates to a microecological preparation and a preparation method and application thereof. Background technique [0002] Eggs are an important source of nutrition in people's daily life. Adding antibiotics to feed can promote the growth of livestock and poultry, not only can prevent livestock and poultry diseases, but also improve the metabolism of livestock and poultry, promote the growth of livestock and poultry, and improve the survival rate and feed conversion rate. However, long-term or indiscriminate use of antibiotics will cause imbalance of the flora in the body, destroy the microecological balance, inhibit the growth and reproduction of sensitive bacteria, and multiply insensitive bacteria, thereby causing endogenous infections in livestock and poultry. Antibiotics will also destroy the third bacteria in the body, creating a large number of vacancies on the att...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K50/75A23K10/18C12R1/125C12R1/10
CPCC12N1/20A23K50/75A23K10/18
Inventor 龚萍杨宇叶胜强钱运国杨永平陈星王丽霞童新红李中心刘武凌明湖杜康裕
Owner WUHAN ACADEMY OF AGRI SCI
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