Preparation method of injectable children's epiphyseal plate regeneration hydrogel

An injection type, regenerated water technology, applied in the field of biomedical materials, can solve the problems of uncontrollable longitudinal growth of long bones, low efficiency of scaffolding into cartilage, and no layered structure, so as to be beneficial to popularization and use, and to promote the regeneration and repair of epiphyseal plate cartilage , The effect of great market application potential

Active Publication Date: 2021-12-10
NANJING CHILDRENS HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a lot of research has been done before, using different seed cells, screening different types of bioactive materials and regulation methods, but still facing the problems of low scaffolding cartilage formation efficiency and poor fusion of regenerated cartilage and surrounding normal tissues
More importantly, the regenerated cartilage does not have a layered structure similar to the natural epiphyseal plate, and cannot control the longitudinal growth of long bones

Method used

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  • Preparation method of injectable children's epiphyseal plate regeneration hydrogel
  • Preparation method of injectable children's epiphyseal plate regeneration hydrogel
  • Preparation method of injectable children's epiphyseal plate regeneration hydrogel

Examples

Experimental program
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Embodiment 1

[0030] 1) Preparation of a sodium alginate double-layer drug-loaded microsphere

[0031] In this embodiment, the preparation of sodium alginate double-layer drug-loaded microspheres is mainly carried out by using the flow focusing method and the microfluidic control method, and the main steps are as follows:

[0032] 1. If figure 2 As shown, the microfluidic chip is placed on the stage of the optical microscope, and six flexible tubes are respectively connected to extend out the six channel ports;

[0033] 2. Add the 1% sodium alginate solution with IGF-1 into the single-channel syringe pump through the syringe, exhaust the air, and connect it to the No. 2 channel port through the hose;

[0034] 3. Draw 1% sodium alginate solution into two syringes, place them in a dual-channel syringe pump, and connect them to the two No. 1 channel ports through a hose;

[0035] 4. Use two syringes to draw 1% CaCl 2 The paraffin oil is placed in a dual-channel syringe pump and connected t...

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Abstract

The invention discloses a preparation method of an injectable hydrogel for children's epiphyseal plate regeneration. The main steps are: 1. The outer layer is an anti-bone bridge formation factor and the inner layer is a cartilage differentiation-promoting factor by using a flow focusing method and a microfluidic control method. 2. Mix SA microspheres with bone marrow mesenchymal stem cells in hydrogel to prepare bioink. The hydrogel is injected into the epiphyseal plate cartilage injury, through the local, timed and quantitative release of Enbrel ® +Bevacizumab and IGF‑1 can inhibit the formation of bone bridge and then promote the regeneration and repair of epiphyseal cartilage, providing new ideas and methods for solving the problem of "repair of epiphyseal injury".

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of an injectable children's epiphyseal plate regeneration hydrogel. Background technique [0002] Physeal plates, also known as growth plates, are areas of cartilage at the ends of immature long bones in children that control bone longitudinal growth. Fractures, infections, tumors, or iatrogenic injuries can all lead to damage to the epiphyseal plate. About 30% of children with fractures are accompanied by damage to the epiphyseal plate and bone growth imbalance. Once the epiphyseal plate is damaged, the cartilage tissue of the epiphyseal plate will be gradually replaced by bone tissue, forming a "bone bridge", leading to growth retardation, angulation or rotation deformity, and even further affecting joint function, which will have a huge impact on children's physical and mental health. The current clinical treatment method is mainly bone bridg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/50A61L27/52A61L27/54
CPCA61L27/3834A61L27/3852A61L27/20A61L27/50A61L27/52A61L27/54A61L2400/06A61L2430/06A61L2300/414A61L2300/624C08L5/04
Inventor 郑朋飞王金武柳毅浩强磊王彩萍
Owner NANJING CHILDRENS HOSPITAL
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