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A reagent red blood cell preservation system and its preparation method

A technology of reagent red blood cells and red blood cells, which is applied in the medical field, can solve problems that have not been reported, and achieve the effects of good antigen stability, wide application prospects, and high morphological ratio

Active Publication Date: 2021-10-08
SHANGHAI BLOOD CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] About a kind of reagent erythrocyte preservation system of the present invention and its preparation method have not yet been reported

Method used

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  • A reagent red blood cell preservation system and its preparation method
  • A reagent red blood cell preservation system and its preparation method
  • A reagent red blood cell preservation system and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 effect experiment

[0033] Erythrocytes are a special type of cells. Mature erythrocytes do not have a nucleus, so their metabolism depends entirely on their own glycolysis for energy, and as the storage time increases, the metabolism of energy substances will lead to changes in the membrane structure of erythrocytes. This change in membrane structure will, on the one hand, lead to changes in the permeability of the red blood cell membrane and changes in cell morphology; on the other hand, it will also lead to changes in the spatial conformation of membrane proteins, leading to changes in antigenicity. Therefore, this embodiment aims to objectively reflect the effect of the reagent erythrocyte preservation solution on the preservation of the reagent erythrocytes to the evaluation of the cell membrane structure and antigenicity.

[0034] 1 Experimental method

[0035] 1.1 Reagent erythrocyte preservation solution (reagent erythrocyte preservation solution, ...

Embodiment 2

[0069] Embodiment 2 concentration gradient test

[0070] 1 Experimental method

[0071] Prepare 0.7; 0.8; 0.9; 1; 1.2; 1.5X preservation solution (optimum concentration is 1), and carry out 3% cell ratio and 15% cell ratio preservation experiments. The 7th day and 75 days, the midpoint of the planned storage period of 3% and 15% cells, were selected as two detection nodes to evaluate the short-term and long-term effect of the gradient on the cells.

[0072] 2 results

[0073] Observation of storage appearance: From the storage appearance of 7 days and 75 days, the storage effect of 1X storage solution is the best, which is the best concentration (see Figure 5 ).

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Abstract

The invention relates to a reagent erythrocyte preservation system and a preparation method thereof. The composition and content of the reagent erythrocyte preservation system are: citric acid-1 water 0.400g / L; trisodium citrate-2 water 6.100g / L ; Glucose 20.500g / L; Inosine 0.350g / L; Chloramphenicol 0.143g / L; Neomycin sulfate 0.143g / L; NaCl 4.200g / L; Adenine 0.500g / L; ; Potassium dihydrogen phosphate 3.000g / L. It has been verified by experiments that using the reagent erythrocyte preservation system of the present invention at 2-8°C, 3% reagent erythrocytes can be effectively preserved for 90 days, and 15% reagent erythrocytes can be effectively preserved for 60 days. The advantages are as follows: the reagent erythrocytes obtained by using the preservation system have a long validity period, a high proportion of normal-appearing erythrocytes, and good antigen stability.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a reagent red blood cell preservation system and a preparation method thereof. Background technique [0002] Erythrocyte blood group antigens are usually single or multiple transmembrane glycoproteins. In terms of expression, blood group antigen proteins can be divided into expression proteins directly encoded by nuclear genes and adsorption proteins; in terms of relationship with cell membrane components, some of these proteins are anchoring proteins that constitute the cytoskeleton, and some are anchoring proteins through binding with anchoring proteins. The interaction is more stable and binds to the membrane, and some only rely on the interaction with the phospholipid layer to bind to the membrane. Studies have shown that the structure and stability of erythrocyte membranes and membrane proteins will change with the increase of storage time. Blood transfusion laboratories u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0221A01N1/0226
Inventor 赵俸涌向东郭忠慧朱自严钱呈睿刘李栋
Owner SHANGHAI BLOOD CENT
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