Vitality construction and standardized preparation method of a direct-throwing type yogurt starter

A starter and direct-injection technology, applied in biochemical equipment and methods, dairy products, testing dairy products, etc., can solve problems such as differences in acid production efficiency, unstable fermentation, and unstable strain viability and activity, and achieve a single The effect of stable bacterial count, good starter activity, total activity of starter and stable bacterial count

Active Publication Date: 2021-06-08
QINGDAO GENYUAN BIOLOGICAL TECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of unstable bacterial activity, unstable fermentation, and differences in texture, taste, flavor and acid production efficiency of different batches of yogurt direct-injection yogurt starter in the process of preparation and use , the present invention provides a direct-throw type yogurt starter vigor construction and standardized preparation method, the adopted technical scheme is as follows:

Method used

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  • Vitality construction and standardized preparation method of a direct-throwing type yogurt starter
  • Vitality construction and standardized preparation method of a direct-throwing type yogurt starter
  • Vitality construction and standardized preparation method of a direct-throwing type yogurt starter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example provides a method for preparing a direct-injection yogurt starter. The starter is characterized by rapid acid production, sweet fruit aroma, and a conventional fermented yogurt texture. The starter contains the main zymomonas Streptococcus thermophilus, which has fast acid production and a sweet fruity aroma, and the subsidiary zymomonas Lactobacillus bulgaricus is added as a yogurt product standard. The specific steps are as follows:

[0047] S1. Determine the standard activity unit of starter fermentation: the standard activity unit of yogurt starter is determined by the specificity of the production level of the main zymomonas Streptococcus thermophilus freeze-dried powder as 1×10 6 cfu / g;

[0048] S2. Determination of the negative activity unit interval of the required main zymomonas: according to the standard activity unit U of the starter SD =1×10 6 cfu / g for inoculation fermentation. Test Streptococcus thermophilus at 0.1U SD , 0.2U SD , 0.3U S...

Embodiment 2

[0057] This example provides a method for preparing a direct-injection-flavored fermented milk starter. The starter is characterized by fast acid production, high curd hardness, high viscosity, and the flavor of conventional fermented yogurt. The starter contains the main zymomonas Streptococcus thermophilus, which has the fermentation performance of high curd hardness and high viscosity, the main zymomonas Lactobacillus bulgaricus has the fermentation performance of fast acid production, and the subsidiary zymomonas Bifidobacterium lactis Added as a probiotic strain, the specific steps are as follows:

[0058] S1. Determine the fermentation standard activity unit of the starter: the production level of the main zymomonas Streptococcus thermophilus and Lactobacillus bulgaricus freeze-dried powder is specific, and the standard of the flavor fermented milk starter is determined by the freeze-dried powder of Lactobacillus bulgaricus with a lower production level Vitality unit is ...

Embodiment 3

[0069] This example provides a method for preparing a direct-throwing curd cheese starter. The starter is characterized by a strong cheese aroma, a fast acid production rate, and a general yogurt texture. The starter contains the main zymomonas Lactococcus lactis subsp. lactic acid, which has a sour cheese aroma, and the main zymomonas Lactococcus lactis subsp. milkfat, which has a sweet cheese aroma, and does not contain subsidiary zymomonas. The specific steps are as follows:

[0070] S1. Determining the fermentation standard activity unit of the starter: determined specifically by the production level of the freeze-dried powder of Lactococcus lactis subsp. The standard vigor unit of curd cheese starter is 6×10 6 cfu / g.

[0071] S2. Determination of the negative activity unit interval of the required main zymomonas: according to the standard activity unit U of the starter SD =6×10 6 cfu / g for inoculation fermentation. First test Lactococcus lactis subsp. lactis at 0.2U ...

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Abstract

The invention discloses a direct-throw type yogurt starter vigor construction and standardized preparation method, and belongs to the technical field of dairy starter preparation. The method is to specifically determine the activity unit of the starter according to the production level of one or more characteristics of the fermentation characteristics, texture and flavor characteristics of the target product yogurt produced by the selected main zymomonal bacteria; To the range of activity units, the amount of fermentation strains to be used is determined according to the range of activity units of the starter and the range of negative activity units of the main zymomonas, and finally compounded to obtain a direct-injection yogurt starter. By standardizing and quantifying the total number of inoculated bacteria and the total inoculated activity of the starter, the method stabilizes the activity of each single bacterium in the starter, the number of each single bacterium, the ratio of the number of main single bacteria, the total activity of the starter and the number of bacteria. Stable, and then the prepared starter has good activity, stable fermentation, stable acid production rate curve between batches, stable yogurt texture, flavor and taste.

Description

technical field [0001] The invention relates to a direct-throw type yogurt starter vigor construction and standardized preparation method, belonging to the technical field of dairy starter preparation. Background technique [0002] Yogurt refers to fresh milk or reconstituted milk that undergoes microbial fermentation, decomposes and uses protein to metabolize lactose in milk into lactic acid, lowers the pH value of milk to below 4.5, and makes casein reach the isoelectric point when the acidity is above 70°T. Coagulation precipitates to form an acidic curd-like product of specific hardness, consistency and flavour. At present, dairy products with yogurt as the main ingredient include yogurt, flavored fermented milk, lactic acid bacteria beverage, fermented milk beverage, and cheese. The microorganisms involved in fermentation to form yogurt are mainly lactic acid bacteria. Lactic acid bacteria is a general term for bacteria that can ferment carbohydrates into lactic acid,...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A23C9/12A23C19/00C12N1/20C12R1/46C12R1/225C12R1/245C12R1/01C12R1/23C12R1/25G01N33/04
CPCA23C9/12A23C19/00C12N1/20G01N33/04A23V2400/147A23V2400/143A23V2400/157A23V2400/165A23V2400/125A23V2400/123A23V2400/519A23V2400/175A23V2400/113A23V2400/249A23V2400/169
Inventor徐显睿张兰威杨洪来李翠凤夏文洋李道河隋勇军陈政言张宗国
OwnerQINGDAO GENYUAN BIOLOGICAL TECH GRP