Reagent for fecal microorganism detection and application of reagent to fecal bacteroides detection

Abstract

The invention discloses a reagent for fecal microorganism detection and an application of the reagent to fecal bacteroides detection. The reagent for fecal microorganism detection comprises an upstream primer with a sequence as shown in Seq ID No. 1 and a downstream primer with a sequence as shown in Seq ID No. 2. According to the reagent for fecal microorganism detection, fecal bacteroides can beaccurately and effectively detected from nucleic acid extracted from a fecal sample, so that whether the fecal sample contains the fecal bacteroides or not is accurately and effectively judged, and the problems of missing detection or false negative and the like caused by instability of fecal nucleic acid extraction are reduced or avoided. The clinical use requirements can be better met.

Description

technical field [0001] The present application relates to the field of fecal microorganism detection, in particular to a reagent for fecal microorganism detection and its application in the detection of fecal Bacteroides. Background technique [0002] There are a large number of different types of microorganisms living in the human intestine. The microecological membrane barrier formed by these microorganisms plays an important role in maintaining the normal immune defense function of the human body. Gut microbes are the largest and most complex microecosystem in the human body. Gut microbes and their metabolites can regulate human health and are important reference indicators for human health or disease. [0003] At present, the detection of intestinal microorganisms is mainly based on fecal microorganisms, that is, the genomic nucleic acid in feces is extracted, and the microbial genome is detected and analyzed. However, the extraction efficiency of genomic nucleic acid ...

AI Technical Summary

Problems solved by technology

However, the extraction efficiency of genomic nucleic acid in the existing qualitative or quantitative detection of intestinal microorganisms is generally low, and the stability is poor; so that the detection effect of fecal microorganisms is poor, especially in some special cases, the detection of some important microbial indicators Cannot meet the needs of clinical analysis

Moreover, the existing microbial genome detection process mainly analyzes and detects a variety of genomic microorganisms in the sample to be tested, and lacks targeted detection of some important microorganisms; coupled with the instability of nucleic acid extraction, it is easy to cause missed detection or false negative

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