Full-automatic sample application device for biological chip, and using method thereof

A technology of biochips and spotting devices, which is applied to the analysis of materials and instruments, and can solve problems such as reagent dripping, contamination of the spotting area, bacteria and dust falling in, etc.

Inactive Publication Date: 2021-01-22
郑州佐爵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technique refers to the immobilization of a large number of probe molecules on the support and hybridization with fluorescently labeled DNA or other sample molecules (such as proteins, factors or small molecules), and then obtains by detecting the hybridization signal intensity of each probe molecule. The number and sequence information of sample molecules; because the overall volume of the biochip is small, when the traditional spotting device is spotting it, there is only one camera to observe the spotting process, so that the spotting needle cannot be accurately placed. Observing the specific spotting position will cause the reagents to drop to other positions. At the same time, during the entire spotting process, the whole device is in an open state, and bacteria and dust are likely to fall into the spotting area, which will cause the spotting area to be damaged. Pollution

Method used

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  • Full-automatic sample application device for biological chip, and using method thereof
  • Full-automatic sample application device for biological chip, and using method thereof
  • Full-automatic sample application device for biological chip, and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Such as figure 1 , figure 2 , image 3 , Figure 5 , Figure 6 , Figure 7 , Figure 8 As shown, the moving mechanism 4 includes a moving frame 41, a moving motor 42, a moving screw 43, a sprocket 44, and a chain 45. The moving motor 42 is connected to the rear end of the isolation box 12 by bolts near the positions on both sides. The ends are connected to the inside of the isolation box 12 by bearings near the two sides, the lower end of the mobile frame 41 is installed on both sides of the top of the base 2, the sprocket 44 is connected to the rear side of the moving screw 43 by a key, and the chain 45 is arranged on the outside of the sprocket 44. Utilize the mobile motor 42 rotating parts to drive the mobile screw 43 on one side of the base 2 to rotate, drive the mobile screw 43 on the other side of the base 2 to rotate by the transmission of the sprocket 44 and the chain 45, and utilize two mobile screw 43 The rotation to drive the moving frame 41 to move fo...

Embodiment 2

[0046] Such as Figure 4 As shown, the difference between Embodiment 2 and Embodiment 1 is that the moving mechanism 4 includes a moving frame 41, a moving motor 42, a moving screw 43, a pulley 414, and a belt 415, and the moving motor 42 is connected to the isolation box 12 rear end by bolts. Near the two sides, the front and rear ends of the moving screw 43 are connected to the inside of the isolation box 12 by bearings, the lower end of the moving frame 41 is installed on both sides of the top of the base 2, and the pulley 414 is connected behind the moving screw 43 through a key. side, the belt 415 is arranged on the outside of the pulley 414, and the moving screw 43 on one side of the base 2 is driven by the rotating part of the moving motor 42 to rotate, and the moving screw on the other side of the base 2 is driven by the transmission of the pulley 414 and the belt 415 43 rotates, utilizes the rotation of two moving lead screws 43 to drive moving frame 41 to move back a...

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Abstract

The invention discloses a full-automatic sample application device for a biological chip, and a using method of the full-automatic sample application device. The full-automatic sample application device comprises a base, an adjusting mechanism, a moving mechanism used for adjusting front-back distance of sample application, and a lifting mechanism. According to the invention, pure air is suppliedinto an isolation box through a fan and a sterile filter screen plate, so that the sample application device in the isolation box is located in a sterile environment, and pollution caused by entranceof other miscellaneous bacteria is prevented; the sample application process is shot and transmitted to a touch display screen to be displayed through three sets of cameras installed on an adjusting frame, so that the whole sample application process can be observed and supervised at multiple positions on the touch display screen so as to prevent liquid from dropping to other positions during sample application; and by using a feeding mechanism, a biochip needing sample application can be fed into an isolation mechanism, so that the biochip does not need to be manually fed into an area with pressure higher than external pressure.

Description

technical field [0001] The invention relates to the field of biochip spotting, in particular to a fully automatic spotting device for biochips and an application method thereof. Background technique [0002] Biochips, also known as protein chips or gene chips, originated from the crystallization of the combination of DNA hybridization probe technology and semiconductor industry technology. This technique refers to the immobilization of a large number of probe molecules on the support and hybridization with fluorescently labeled DNA or other sample molecules (such as proteins, factors or small molecules), and then obtains by detecting the hybridization signal intensity of each probe molecule. The number and sequence information of sample molecules; because the overall volume of the biochip is small, when the traditional spotting device is spotting it, there is only one camera to observe the spotting process, so that the spotting needle cannot be accurately placed. Observing ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N35/10G01N35/00
CPCG01N35/00029G01N35/1011G01N2035/00158
Inventor王利利鄂光照
Owner郑州佐爵生物科技有限公司