Fixed bed bioreactor

Abstract

The invention provides a fixed bed bioreactor, and belongs to the technical field of cell culture. The bioreactor comprises a tank body (6); a tank cover (5) is arranged at an upper port of the tank body (6); and a micro-carrier (9) is fixed in the tank body (6). The bioreactor is characterized in that a placing tower (8) is arranged in the tank body (6); the micro-carrier (9) is placed on an outer ring of the placing tower (8); circulating channels of a culture medium are formed between the placing tower (8) and the inner wall of the tank body (6) as well as inside the placing tower (8); anda circulation mechanism (10) for circulating the culture medium inside the circulating channels is arranged at the bottom of the placing tower (8). In the fixed bed bioreactor, the circulating mechanism is arranged, and the culture medium is circulated in the tank body by virtue of the circulating mechanism, so that when the culture medium flows upwards from the bottom of the micro-carrier, the carrier is loosened by virtue of the flowing impact force of the culture medium, and cells can absorb nutrients more favorably.

Description

technical field [0001] A fixed-bed bioreactor belongs to the technical field of cell culture. Background technique [0002] Fixed bed bioreactor is a new type of cell culture device, which can realize up to 10 8 The high-concentration cell culture level per mL is about 10 times that of other conventional methods (suspension culture). The existing fixed bed reactor includes a carrier fixing device and a liquid storage tank. The medium is pumped to the top of the fixed bed through a peristaltic pump, and the medium permeates through the fixed bed and returns to the liquid storage tank from the bottom of the fixed bed, and the cycle is repeated in turn. . In this culture method, the medium newly entering the fixed bed cannot be fully mixed with the original medium in the fixed bed, and the accumulation of the carrier causes the cells inside the carrier to fail to fully absorb nutrients, resulting in slow cell growth and low cell density after the end of the culture. , affect...

AI Technical Summary

Problems solved by technology

In this culture method, the medium newly entering the fixed bed cannot be fully mixed with the original medium in the fixed bed, and the accumulation of the carrier causes the cells inside the carrier to fail to fully absorb nutrients, resulting in slow cell growth and low cell density after the end of the culture. , affecting the quality of cell culture

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