33 results about "Fixed bed bioreactor" patented technology

The present invention relates to a novel approach for nitrate removal from a marine recirculating system (10) wherein high concentrations of sulfate found in seawater is used in combination with sludge (20) collected from fish growing tanks (12) to promote dissimilatory sulfate reduction to hydrogen sulfide. The sulfide is used as an electron source to promote autotrophic denitrification in an up-flow fix bed bioreactor (16), followed by nitrification in a nitrification unit (14). By utilizing the symbiotic relationship between the sulfate-reducing and sulfide-oxidizing bacterial community, nitrate accumulation is controlled in the recirculating water of the system thereby reducing water exchange in the marine recirculating system.

A method and a plant to remove metals by biosorption from mining or industrial effluents comprising: (a) subject the effluent to at least one first stage of pre-treatment, selecting among: precipitation by pH rising, solvent extraction or solvent extraction on emulsified membranes in order to reduce its load of metals to a concentration level that allows it to be treated by the next stage of biosorption, and (b) subject the liquid that has been previously in the stage of pre-treatment to a second stage of continuous metals removal by biosorption. To perform the continuous metals removal by biosorption is supplied a group of reactors, constituted for at least one, or at least two or at least three fixed bed bioreactors, which packing material has been colonized with a biofilm formed by a bacterial culture or a mixture of microorganisms with the capacity of binding metals by biosorption; pre-treated water is led to the group of bioreactors, the treated effluent comes from this last one and is led to the discharge of the plant.

The present disclosure relates to methods of producing Enterovirus C, e.g., for poliomyelitis vaccine production. In some embodiments, the methods include adding polysorbate to the cell culture medium during or prior to inoculation with the virus and / or culturing cells in a fixed bed bioreactor. Further provided herein is an Enterovirus C produced by the methods of production disclosed herein, as well as compositions, immunogenic compositions, and vaccines related thereto.

The present invention may be used in methods for treatment of drinking water contaminated with algal metabolites. An inflow of water in a drinking water treatment facility may have multiple types of algal metabolites wherein each algal metabolites has a concentration of less than 20 μg / l. The water may contain indigenous bacteria that may serve as a bioreactor inoculum. The water may be dosed with a biodegradable electron donor at a concentration of less than 7 mg / l to form water, electron donor solution. The water, electron donor solution may be processed through a fixed-bed bioreactor for an empty bed bioreactor contact time of less than 30 minutes. An effluent of the fixed-bed bioreactor may have an algal metabolite concentration for each algal metabolite of less than 10 ng / l.

A method and a plant to remove metals by biosorption from mining or industrial effluents comprising: (a) subject the effluent to at least one first stage of pre-treatment, selecting among: precipitation by pH rising, solvent extraction or solvent extraction on emulsified membranes in order to reduce its load of metals to a concentration level that allows it to be treated by the next stage of biosorption, and (b) subject the liquid that has been previously in the stage of pre-treatment to a second stage of continuous metals removal by biosorption. To perform the continuous metals removal by biosorption is supplied a group of reactors, constituted for at least one, or at least two or at least three fixed bed bioreactors, which packing material has been colonized with a biofilm formed by a bacterial culture or a mixture of microorganisms with the capacity of binding metals by biosorption; pre-treated water is led to the group of bioreactors, the treated effluent comes from this last one and is led to the discharge of the plant.

The shell-and-tube fixed bed bioreactor includes one upper end sealing body with liquid inlet; one reaction cavity with stuffing pipes filled with solid stuffing; one lower end sealing body; and one distributor in the upper end of the stuffing pipes with homogeneously distributed through holes for the liquid from the upper end sealing body to be sprayed homogeneously to inside the stuffing pipes. The shell-and-tube fixed bed bioreactor has simple structure, easy operation, high yield and high resource utilizing rate.

Mesophilic methane bacteria obtained from digested sludge are acclimatized in an H2 / CO2 medium and the acclimatized methane bacteria are grown on a support inclusive of inorganic nutritional salts of trace metal elements by using an immobilized bed bioreactor. The grown bacteria are allowed to metabolize a mixed gas, prepared by adding hydrogen to at least one of coal gas and biogas, into methane and at the same time, cobalamin contained in the fermented liquor is recovered as an extracellular product in the form of cyanocobalamin by using potassium cyanide to thereby produce vitamin B12 efficiently in a high content and high yield.

Fixed bed bioreactor with natural convection and forced draught to obtain bioactive substances by solid-state fermentation (SEF) using fungi macromycetes. This bioreactor may be from turn drum with pendulum motion and natural and forced convection to a tray bioreactor with natural convection. They are used in the production of bioactive substances as crude extracts of lignocellulosic enzymes and fungal polysaccharides obtained by using mixtures of lignocellulosic materials as substrates and macromycetes fungi as inoculum, controlling pH, humidity and particle size, inoculation rate and environmental conditions during fermentation such as temperature, relative humidity and carbon dioxide and oxygen concentration.

The present invention relates to methods of producing Enterovirus A, e.g., for vaccine production, that include culturing cells in a fixed bed bioreactor. Further provided herein are an Enterovirus Aproduced by the methods of production disclosed herein, as well as compositions, immunogenic compositions, and vaccines related thereto.

The present invention discloses a bioartificial liver system comprising a fixed-bed bioreactor packed with gel beads containing hepatocytes of an animal, a plasma reservoir, a plasma separator, and an efflux chamber, wherein the plasma reservoir is located at a position higher than the bioreactor, a ventilation filter in contact with atmosphere is provided at the top of the plasma reservoir, and the top of the plasma reservoir is directly connected to the efflux chamber via a connecting line. The inventive system satisfactorily performs the essential liver functions without the problems of gel bead damage or channeling.

The present invention may be used in methods for treatment of drinking water contaminated with algal metabolites. An inflow of water in a drinking water treatment facility may have multiple types of algal metabolites wherein each algal metabolites has a concentration of less than 20 μg / l. The water may be dosed with a biodegradable electron donor at a concentration of less than 7 mg / l to form a water, electron donor solution. The water, electron donor solution may be processed through a fixed-bed bioreactor for an empty bed bioreactor contact time of less than 30 minutes. An effluent of the fixed-bed bioreactor may have an algal metabolite concentration for each algal metabolite of less than 10 ng / l.

The shell-and-tube fixed bed bioreactor includes one upper end sealing body with liquid inlet; one reaction cavity with stuffing pipes filled with solid stuffing; one lower end sealing body; and one distributor in the upper end of the stuffing pipes with homogeneously distributed through holes for the liquid from the upper end sealing body to be sprayed homogeneously to inside the stuffing pipes.The shell-and-tube fixed bed bioreactor has simple structure, easy operation, high yield and high resource utilizing rate.

The present invention may be used in methods for treatment of drinking water contaminated with algal metabolites. An inflow of water in a drinking water treatment facility may have multiple types of algal metabolites wherein each algal metabolites has a concentration of less than 20 μg / l. The water may contain indigenous bacteria that may serve as a bioreactor inoculum. The water may be dosed with a biodegradable electron donor at a concentration of less than 7 mg / l to form water, electron donor solution. The water, electron donor solution may be processed through a fixed-bed bioreactor for an empty bed bioreactor contact time of less than 30 minutes. An effluent of the fixed-bed bioreactor may have an algal metabolite concentration for each algal metabolite of less than 10 ng / l.

The invention provides a hollow tube bundle type fixed bed bioreactor for adherent culture of mammalian cells, and can effectively solve the technical problems that cells are unevenly distributed on a carrier, the center of a carrier stack is prone to produce a cell-free "dead zone", shear force of a fluid medium in the reactor is large, and the cells are difficult to harvest. The hollow tube bundle type fixed bed cell bioreactor comprises a tank body, a carrier, a culture medium driving unit and a gas supply unit; a flange cover is mounted at the top of the tank body, and the carrier is arranged in the tank body. The hollow tube bundle type fixed bed cell bioreactor is characterized in that only flow channels vertical and evenly distributed are arranged in the carrier; gaps are arranged between the upper end of the carrier and the flange cover and between the carrier and the side wall of the tank body; and culture mediums in the tank body flow in from the opening at lower ends of the flow channels under the action of the culture medium driving unit, and then return to the bottom of the tank body after sequentially passing through the flow channels, the gap between the carrier and the flange cover and the gap between the carrier and the side wall of the tank body.

The invention discloses a carrier fixed bed bioreactor and a water treatment system comprising the carrier fixed bed bioreactor. The carrier fixed bed bioreactor comprises a tank body and a fillter; alower-layer hole net is arranged at the lower part of the tank body and is used for supporting a filler, a plurality of lower holes penetrating through the lower-layer hole net in the thickness direction are formed in the lower-layer hole net, and the diameter of the lower holes are designed to be smaller than the diameter of the filler; an upper-layer hole net is arranged at the upper part of the tank body and is used for blocking the filler, a plurality of upper holes penetrating through the upper-layer hole net along the thickness direction are formed in the upper-layer hole net, and the diamerter of the upper holes is designed to be smaller than that of the filler; the filler is kept in a space defined by the lower-layer hole net, the upper-layer hole net and the corresponding side walls of the tank body, and the total volume of the filler is smaller than the volume of the space, so that when the filler is deposited on the lower-layer hole net, the top of the filler is separated from the upper-layer hole net, wherein the density of the filler is slightly larger than that of water, so that the filler is deposited on the lower-layer hole net when water introduction is stopped, and the filler floats and is blocked by the upper-layer hole net when the flow speed of introduced water reaches a preset flow speed or above.