23 results about "Fixed bed bioreactor" patented technology

The utility model discloses a waterfall flow type stackable fixed bed bioreactor which comprises a tank body, a baffle plate is arranged on the inner wall of the tank body, and a cover plate is arranged at the upper end of the tank body in a sealing manner; a shaft seal is rotatably arranged in the middle of the cover plate, a hollow stirring shaft is fixedly arranged at the lower end of the shaft seal, a lower stirrer is fixedly arranged at the tail of the other end of the stirring shaft, and an upper stirrer is arranged in the middle of the stirring shaft; the upper stirrer and the lower stirrer rotate to generate waterfall flow; the fixed bed is used for culturing and fixing the cells. A packaged fiber membrane is arranged in the middle of the fixed bed. The fixed bed bioreactor solves the problems of non-uniform distribution, non-synchronous growth and expression and the like of cells in the traditional fixed bed bioreactor; due to the stirring design, the mass transfer performance is improved, and better growth conditions are given to cells; the waterfall flow design improves the uniformity when the cells are inoculated to the fixed bed, provides possibility for subsequent consistent growth and expression, facilitates cell digestion operation, is milder in reaction, and saves pancreatin, serum and the like.

The utility model relates to the technical field of bioreactors, and discloses a novel fixed bed bioreactor which comprises a tank body, an end cover is arranged at the top end of the tank body, a culture assembly is arranged in the tank body, the culture assembly comprises a support, the support is installed at the bottom of the tank body, a roll film is arranged in the support, and a stirring rotor is arranged in the support and located below the roll film; a plurality of filters communicated with the interior of the tank body are arranged on the side edge of the tank body, and a stirring main machine for driving the stirring rotor is arranged below the tank body. The fixed bed bioreactor is simple in structure, easy and convenient to operate and wide in application range, the advantages of a three-dimensional culture system are fully utilized, and high-yield biological products are obtained.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

A bioreactor system for culturing cells is provided that include a cell culture vessel having an inlet, outlet, and interior reservoir. The cell culture matrix includes a plurality of substrates for adhering cells thereto, which are stacked in a stacking direction parallel to a flow direction through the reservoir. The cell culture matrix extends across the width of the reservoir such that fluid flow between the cell culture matrix and the interior wall is restricted. The substrates can be under compression with a compression force in a direction that is substantially parallel to the stacking direction and substantially perpendicular to the reservoir width to reduce bypass fluid flow between the cell culture matrix and the reservoir wall. Edges of some of the substrates can also be joined to increase rigidity against fluid flow and thus reduce bypass.

This invention relates to the field of biopharmaceutical manufacturing technology, and discloses a pine needle extract scalp health repair agent and its preparation method, comprising: using a fixed-bed bioreactor to set an average residence time that simultaneously satisfies the desired reaction and avoids the generation of impurities; and by actively applying transient flow rate disturbances and analyzing the product concentration response, diagnosing the inactivation state of the bioconversion enzyme system online, and compensatingly reducing the flow rate accordingly. This invention avoids the generation of immunogenic impurities; at the same time, by actively diagnosing and compensating for enzyme inactivation, it solves the fundamental problem of yield decline in continuous production, ensuring the absolute stability of the process in long-term operation.

A cell culture bioreactor is provided that includes a cell culture vessel with a vessel wall, having an interior-facing surface and an exterior facing surface. A substrate is also provided, arranged as a fixed bed within the cell culture vessel and surrounded by the vessel wall. The substrate is designed for adhering cells thereto for cell culture. The bioreactor includes at least one biomass sensor within the cell culture vessel. The biomass sensor includes at least one conductor that can generate an electric field passing through at least a portion of the fixed bed. The at least one biomass sensor is designed to measure at least one of capacitance and impedance within the cell culture vessel.

A bioreactor for culturing and harvesting cells is provided. The bioreactor includes a vessel with an interior cavity and one or more media ports for passing fluid between the interior cavity and an exterior of the vessel, and a substrate disposed in the interior cavity and having a surface for adhering cells thereto. The bioreactor also includes an agitator arranged to transmit mechanical energy to at least one of the vessel, the substrate, and a fluid in the interior cavity. The agitator transmits mechanical energy sufficient to contribute to either detaching cells adhered to the substrate or dislodging cells entrapped in the substrate.

The present invention discloses a rolled fixed-bed bioreactor suitable for the production of vaccines and antibodies. A fiber roll rolled from a thermoplastic resin is arranged in a fiber cavity of the fixed-bed reactor. A skeleton support is arranged on the outer transverse end surface of the fiber roll. The lower part of the fiber cavity is connected to an impeller cavity, and an impeller is arranged in the impeller cavity. The present invention transforms the traditional stacked sheet fiber carriers into a rolled fiber carrier fixed bed. The fiber roll makes the direction of the fixed bed matrix the same as the flow direction of the liquid, the liquid circulation during cell inoculation is better, the inoculation uniformity is better, the wall adhesion homogeneity is better during the culture process, the cells have good uniformity in nutrient and oxygen consumption, and the cultured cells can be conveniently sampled by arranging easy-to-tear long fiber strips to observe the growth status of the cells. At the same time, the sampling device is specially designed to greatly reduce the risk of bacterial contamination caused by sampling.

A fixed-bed bioreactor for culturing cells on a cell culture substrate and allowing in- process sampling is provided. The bioreactor includes a cell culture vessel with an interior reservoir in which the fixed bed is disposed. A fixed bed disposed in the interior reservoir includes multiple layers of a cell culture substrate, and one or more sample substrates at least partially disposed between at least some of the multiple layers of the cell culture substrate. The vessel has an opening to the interior reservoir that can be closed during a perfusion cell culture process, but is openable by a user to access the sample substrate during a sampling process, and re-closeable after the sampling process. The sample substrate is removable from the interior reservoir during the sampling process without destroying a remainder of the fixed bed so that the cell culture can continue.

The present invention provides a 3D porous growth surface comprising a fabric with multiple mesh layers, which can be formed into a cylindrical fixed bed by rolling the mesh layers, or other shapes of fixed bed by stacking or filling the multi-layer mesh structure. The present invention also provides a method for enhancing cell growth by maintaining the pore size and structure of the mesh layers. The present invention further provides a method for enhancing cell recovery rate by using the mesh layers described above, which has less obstacles than the carriers made of non-woven fabric or other porous materials, thereby enhancing cell recovery rate. The present invention also provides a method for reducing particle generation during cell collection by sealing the edges of the multi-layer mesh structure to block the release of fibers or particles, and using the yarn diameter of the mesh layers to be larger than the cell wall size for easy filtration separation. The growth surface provided by the present invention can be easily formed into a fixed bed by simply rolling multiple mesh layers, which not only reduces manufacturing costs, but also facilitates mass production of carriers for fixed bed bioreactors.

The utility model relates to the technical field of bioreactors, and discloses a sterile sampler and a sterile sampling fixed bed bioreactor, the bioreactor comprises a tank body, a stirring bin and a culture bin used for installing a fixed bed, the tank body is provided with an upper cover, and the upper cover is provided with a pipeline structure used for being communicated with the interior of the tank body; a consumable replacement opening is formed in the upper cover, a top cover is detachably and hermetically connected to the consumable replacement opening of the upper cover, and the culture bin is connected to the inner side of the top cover; the stirring bin is connected to one side, far away from the top cover, of the culture bin and is suspended in the tank body; a sampling opening is formed in the top cover, and a sterile sampler is arranged at the sampling opening. The consumables and the tank body are relatively independent, the consumables can be mounted without opening the upper cover of the tank body, and a tank body pipeline cannot be influenced; and the stirring device adopts a disposable structure, so that the reduction of foreign matters generated by abrasion is facilitated.

1. The name of this design product: Floor-standing fixed-bed bioreactor. 2. Purpose of the product of this design: A biological production platform for large-scale cell culture and viral vector production and the preparation of other biological macromolecules. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: a three-dimensional diagram of the combined state.

The invention relates to the technical field of sewage treatment, and discloses a microbial carrier and a preparation method thereof. The microbial carrier is of a columnar three-layer structure and sequentially comprises a microbial core, a coating interlayer and an outer supporting layer from inside to outside, the microbial core is a cross-linked porous short fiber column, the coating interlayer is made of fine denier fibers, and the outer supporting layer is made of coarse denier fibers. The pores in the surface of the microbial core can load a large number of microorganisms, it is guaranteed that the microorganisms have high concentration, the middle coating interlayer can intercept organic matter, sufficient nutrients are provided for the microorganisms, meanwhile, the microorganisms are prevented from falling off, the outer supporting layer has a dispersion effect on water flow, the microorganisms are prevented from making direct contact with the water flow, and the water quality is improved. The stable survival of microorganisms is ensured. The microbial carrier disclosed by the invention can be mounted in a honeycomb type fixed bed bioreactor and is used for continuously and efficiently treating sewage and wastewater.

A method for collecting cells from a bioreactor is provided wherein the bioreactor comprises cells to be collected, at least one inlet and / or outlet, and a culture chamber in which the cells are attached to a matrix. The method comprises providing a wash solution having a cell dissociation reagent (CDR) into the culture chamber; and after providing the washing solution, removing and capturing the detached cells from the culture chamber via the at least one inlet and / or outlet. The cell dissociation agent includes an endonuclease, in particular a deoxyribonuclease, such as deoxyribonuclease I (DNase I). The wash solution may further include a divalent metal ion, such as Mg + 2, Ca + 2, or Mn + 2.

A bioreactor system for culturing cells is provided that include a cell culture vessel having an inlet, outlet, and interior reservoir. The cell culture matrix includes a plurality of substrates for adhering cells thereto, which are stacked in a stacking direction parallel to a flow direction through the reservoir. The cell culture matrix extends across the width of the reservoir such that fluid flow between the cell culture matrix and the interior wall is restricted. The substrates can be under compression with a compression force in a direction that is substantially parallel to the stacking direction and substantially perpendicular to the reservoir width to reduce bypass fluid flow between the cell culture matrix and the reservoir wall. Edges of some of the substrates can also be joined to increase rigidity against fluid flow and thus reduce bypass.

The present invention relates to a method for obtaining one or more biomolecules of interest from cells cultured in a fixed bed bioreactor, said method comprising: adding to said bioreactor a lysis solution, thereby lysing said cells in said bioreactor, and before, simultaneously with and / or after said cells are lysed, separating said cells from said cells; one or more mechanical effects are applied to the bioreactor after which the one or more target biomolecules are recovered from the bioreactor. The invention further relates to a system for obtaining one or more target biomolecules from cells cultured in a fixed bed bioreactor.

A fixed bed bioreactor system is provided, the system including a vessel having a media inlet, a media outlet, and an interior chamber disposed between the media inlet and the media outlet and in fluid communication with the media inlet and the media outlet. The vessel further includes a cell culture media positioned in the interior chamber between the media inlet and the media outlet in a packed bed configuration, the cell culture media including a plurality of porous disks in a stacked arrangement. The interior chamber includes a cell culture zone and a spacer zone, the cell culture media defining the cell culture zone, and the spacer zone disposed between the cell culture zone and the media outlet, and each of the plurality of porous disks having a surface configured to culture cells thereon.

A bioreactor system is provided that includes a cell culture vessel having an interior reservoir defined by an interior wall. A fixed-bed cell culture matrix having a plurality of layers of substrate is contained within the interior reservoir and has a bed height and a bed width in a direction perpendicular to the direction of the bed height. The system further includes a compression sleeve surrounding at least a portion of the fixed-bed cell culture matrix. The compression sleeve exerts a compressive force in a direction of the bed width. The compressive force is sufficient to compress the bed width to a compressed bed width that is less than the reservoir width, and the compression sleeve is configured to release the compressive force so that the bed width increases to an expanded bed width.

A cell culture bioreactor is provided that enables in-situ biomass sensing using interdigitated electrodes. The bioreactor includes a cell culture vessel having at least one inlet, at least one outlet, and an interior reservoir disposed between the at least one inlet and the at least one outlet. The bioreactor also includes a fixed bed disposed in the interior reservoir and having a substrate for culturing cells adhered to a surface thereof. The bioreactor further includes at least one biomass sensor, which includes a pair of electrode structures, each having a plurality of interdigitated electrode branches, where the pair of electrode structures are disposed within the fixed bed. The biomass sensor also includes an impedance analyzer electrically coupled to the pair of electrode structures, wherein the biomass sensor can detect a change in impedance between the pair of electrode structures based on changes in the microenvironment surrounding the interdigitated electrodes.

A system for biomass sensing includes a fixed bed bioreactor including a container and a fixed bed disposed within such container. At least one sensor is for sensing one or more parameters representative of biomass in the fixed bed. A controller is adapted for correlating the one or more parameters to an amount of biomass of the fixed bed bioreactor, such as using a correlation model. Related systems and methods are also described.

The utility model relates to the technical field of fixed bed bioreactors, and discloses a fixed bed bioreactor sampling structure, which comprises a plurality of sampling seats communicated with the top of a tank body in a penetrating manner, and one end, positioned outside the tank body, of each sampling seat is connected with a hose; a sampling strip penetrates through the hose, one end of the sampling strip is arranged outside the hose, and the other end of the sampling strip extends into the rolling film in the tank body along the hose; a control part used for clamping or loosening the hose is arranged on the hose, an air inlet is formed in the top of the tank body in a penetrating mode, the air inlet is used for maintaining micro-positive pressure in the tank body, and the air inlet is communicated with an external filtering air pump. One end of the sampling strip is externally arranged, during sampling, the pipe clamp is opened, the end, located outside the hose, of the sampling strip is pulled through a tool, the sampling strip is directly taken out, and then the pipe clamp is clamped, so that sampling is completed, the sampling mode is simple and convenient to operate, meanwhile, the interior of the tank body is micro-positive pressure, external gas can be inhibited from entering the tank body during sampling, and the sampling efficiency is improved. And the internal environment of the tank body is prevented from being damaged.

A bioreactor includes a housing with a number of removable parts for modular construction and easy assembly or disassembly. The removable parts include a fixed bed and a support for the fixed bed. More than one support may be provided. The multiple supports may interlock with each other or a lid of the bioreactor. The support interlocks with the housing to prevent relative rotation. The multiple supports include a support frame for supporting the fixed bed an allowing fluid to flow through the support frame. The fixed bed forms a peripheral chamber between the support and the housing to hold the fixed bed. One or more probes and tubes may be inserted into the bioreactor to test parameters of or to add to or remove liquid from inside.

The invention relates to a large-scale production system and method for mouse leukemia viruses based on a fixed bed bioreactor, and belongs to the technical field of mouse leukemia virus production method.The large-scale production system for the mouse leukemia viruses based on the fixed bed bioreactor comprises a reactor system, a detachable double-layer film cylindrical stainless steel tank body is adopted; the sheet-shaped carrier is fixedly supported by adopting a PET film, a 3D structure and a PP spacing net; the control system adopts a waterfall flow and magnetic suspension straight paddle impeller to transfer oxygen from bottom to top in a waterfall manner, so that the high kLa is realized, and materials are in uniform contact with cells; the pH motor, the DO electrode, the temperature sensor, the pressure sensor and real-time detection are adopted, the yield is increased by multiple times compared with a traditional method, the cost is reduced, the consumption of a culture medium is reduced, and the manual operation time is shortened.