13 results about "Fixed bed bioreactor" patented technology

The utility model relates to the technical field of bioreactors, and discloses a novel fixed bed bioreactor which comprises a tank body, an end cover is arranged at the top end of the tank body, a culture assembly is arranged in the tank body, the culture assembly comprises a support, the support is installed at the bottom of the tank body, a roll film is arranged in the support, and a stirring rotor is arranged in the support and located below the roll film; a plurality of filters communicated with the interior of the tank body are arranged on the side edge of the tank body, and a stirring main machine for driving the stirring rotor is arranged below the tank body. The fixed bed bioreactor is simple in structure, easy and convenient to operate and wide in application range, the advantages of a three-dimensional culture system are fully utilized, and high-yield biological products are obtained.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

A bioreactor system for culturing cells is provided that include a cell culture vessel having an inlet, outlet, and interior reservoir. The cell culture matrix includes a plurality of substrates for adhering cells thereto, which are stacked in a stacking direction parallel to a flow direction through the reservoir. The cell culture matrix extends across the width of the reservoir such that fluid flow between the cell culture matrix and the interior wall is restricted. The substrates can be under compression with a compression force in a direction that is substantially parallel to the stacking direction and substantially perpendicular to the reservoir width to reduce bypass fluid flow between the cell culture matrix and the reservoir wall. Edges of some of the substrates can also be joined to increase rigidity against fluid flow and thus reduce bypass.

This invention relates to the field of biopharmaceutical manufacturing technology, and discloses a pine needle extract scalp health repair agent and its preparation method, comprising: using a fixed-bed bioreactor to set an average residence time that simultaneously satisfies the desired reaction and avoids the generation of impurities; and by actively applying transient flow rate disturbances and analyzing the product concentration response, diagnosing the inactivation state of the bioconversion enzyme system online, and compensatingly reducing the flow rate accordingly. This invention avoids the generation of immunogenic impurities; at the same time, by actively diagnosing and compensating for enzyme inactivation, it solves the fundamental problem of yield decline in continuous production, ensuring the absolute stability of the process in long-term operation.

A cell culture bioreactor is provided that includes a cell culture vessel with a vessel wall, having an interior-facing surface and an exterior facing surface. A substrate is also provided, arranged as a fixed bed within the cell culture vessel and surrounded by the vessel wall. The substrate is designed for adhering cells thereto for cell culture. The bioreactor includes at least one biomass sensor within the cell culture vessel. The biomass sensor includes at least one conductor that can generate an electric field passing through at least a portion of the fixed bed. The at least one biomass sensor is designed to measure at least one of capacitance and impedance within the cell culture vessel.

A bioreactor for culturing and harvesting cells is provided. The bioreactor includes a vessel with an interior cavity and one or more media ports for passing fluid between the interior cavity and an exterior of the vessel, and a substrate disposed in the interior cavity and having a surface for adhering cells thereto. The bioreactor also includes an agitator arranged to transmit mechanical energy to at least one of the vessel, the substrate, and a fluid in the interior cavity. The agitator transmits mechanical energy sufficient to contribute to either detaching cells adhered to the substrate or dislodging cells entrapped in the substrate.

A fixed-bed bioreactor for culturing cells on a cell culture substrate and allowing in- process sampling is provided. The bioreactor includes a cell culture vessel with an interior reservoir in which the fixed bed is disposed. A fixed bed disposed in the interior reservoir includes multiple layers of a cell culture substrate, and one or more sample substrates at least partially disposed between at least some of the multiple layers of the cell culture substrate. The vessel has an opening to the interior reservoir that can be closed during a perfusion cell culture process, but is openable by a user to access the sample substrate during a sampling process, and re-closeable after the sampling process. The sample substrate is removable from the interior reservoir during the sampling process without destroying a remainder of the fixed bed so that the cell culture can continue.

The present invention provides a 3D porous growth surface comprising a fabric with multiple mesh layers, which can be formed into a cylindrical fixed bed by rolling the mesh layers, or other shapes of fixed bed by stacking or filling the multi-layer mesh structure. The present invention also provides a method for enhancing cell growth by maintaining the pore size and structure of the mesh layers. The present invention further provides a method for enhancing cell recovery rate by using the mesh layers described above, which has less obstacles than the carriers made of non-woven fabric or other porous materials, thereby enhancing cell recovery rate. The present invention also provides a method for reducing particle generation during cell collection by sealing the edges of the multi-layer mesh structure to block the release of fibers or particles, and using the yarn diameter of the mesh layers to be larger than the cell wall size for easy filtration separation. The growth surface provided by the present invention can be easily formed into a fixed bed by simply rolling multiple mesh layers, which not only reduces manufacturing costs, but also facilitates mass production of carriers for fixed bed bioreactors.

A method for collecting cells from a bioreactor is provided wherein the bioreactor comprises cells to be collected, at least one inlet and / or outlet, and a culture chamber in which the cells are attached to a matrix. The method comprises providing a wash solution having a cell dissociation reagent (CDR) into the culture chamber; and after providing the washing solution, removing and capturing the detached cells from the culture chamber via the at least one inlet and / or outlet. The cell dissociation agent includes an endonuclease, in particular a deoxyribonuclease, such as deoxyribonuclease I (DNase I). The wash solution may further include a divalent metal ion, such as Mg + 2, Ca + 2, or Mn + 2.

A bioreactor system for culturing cells is provided that include a cell culture vessel having an inlet, outlet, and interior reservoir. The cell culture matrix includes a plurality of substrates for adhering cells thereto, which are stacked in a stacking direction parallel to a flow direction through the reservoir. The cell culture matrix extends across the width of the reservoir such that fluid flow between the cell culture matrix and the interior wall is restricted. The substrates can be under compression with a compression force in a direction that is substantially parallel to the stacking direction and substantially perpendicular to the reservoir width to reduce bypass fluid flow between the cell culture matrix and the reservoir wall. Edges of some of the substrates can also be joined to increase rigidity against fluid flow and thus reduce bypass.

A bioreactor system is provided that includes a cell culture vessel having an interior reservoir defined by an interior wall. A fixed-bed cell culture matrix having a plurality of layers of substrate is contained within the interior reservoir and has a bed height and a bed width in a direction perpendicular to the direction of the bed height. The system further includes a compression sleeve surrounding at least a portion of the fixed-bed cell culture matrix. The compression sleeve exerts a compressive force in a direction of the bed width. The compressive force is sufficient to compress the bed width to a compressed bed width that is less than the reservoir width, and the compression sleeve is configured to release the compressive force so that the bed width increases to an expanded bed width.

A cell culture bioreactor is provided that enables in-situ biomass sensing using interdigitated electrodes. The bioreactor includes a cell culture vessel having at least one inlet, at least one outlet, and an interior reservoir disposed between the at least one inlet and the at least one outlet. The bioreactor also includes a fixed bed disposed in the interior reservoir and having a substrate for culturing cells adhered to a surface thereof. The bioreactor further includes at least one biomass sensor, which includes a pair of electrode structures, each having a plurality of interdigitated electrode branches, where the pair of electrode structures are disposed within the fixed bed. The biomass sensor also includes an impedance analyzer electrically coupled to the pair of electrode structures, wherein the biomass sensor can detect a change in impedance between the pair of electrode structures based on changes in the microenvironment surrounding the interdigitated electrodes.

The invention relates to a large-scale production system and method for mouse leukemia viruses based on a fixed bed bioreactor, and belongs to the technical field of mouse leukemia virus production method.The large-scale production system for the mouse leukemia viruses based on the fixed bed bioreactor comprises a reactor system, a detachable double-layer film cylindrical stainless steel tank body is adopted; the sheet-shaped carrier is fixedly supported by adopting a PET film, a 3D structure and a PP spacing net; the control system adopts a waterfall flow and magnetic suspension straight paddle impeller to transfer oxygen from bottom to top in a waterfall manner, so that the high kLa is realized, and materials are in uniform contact with cells; the pH motor, the DO electrode, the temperature sensor, the pressure sensor and real-time detection are adopted, the yield is increased by multiple times compared with a traditional method, the cost is reduced, the consumption of a culture medium is reduced, and the manual operation time is shortened.