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Detachable fixed bed bioreactor suitable for virus vector production

A bioreactor, dismantling and fixing technology, which is applied to specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, tissue cell/virus culture device, etc. Uneven distribution, low transfection efficiency, etc., to achieve the effect of prolonging the production cycle, uniform cell growth state, and balanced nutrients

Pending Publication Date: 2022-04-29
同腾新创(苏州)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a detachable fixed-bed bioreactor suitable for virus vector production, which solves the problem of exi

Method used

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  • Detachable fixed bed bioreactor suitable for virus vector production
  • Detachable fixed bed bioreactor suitable for virus vector production
  • Detachable fixed bed bioreactor suitable for virus vector production

Examples

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Embodiment 1

[0050] Such as Figure 1-10 As shown, this embodiment provides a detachable fixed-bed bioreactor suitable for the production of viral vectors, including a tank body 5, a culture bin 7, a stirring bin 9, a fixed-bed mechanism 11 and a sampling strip 22, wherein the tank body 5. A culture chamber 7 is installed inside, and a fixed bed mechanism 11 is installed in the culture chamber accommodation portion 71 of the culture chamber 7. Specifically, the fixed bed mechanism 11 includes a first constituent layer 20 and a second constituent layer 21, wherein the first There are at least two constituting layers 20, and a second constituting layer 21 is arranged between adjacent first constituting layers 20, and the first constituting layer 20 and the second constituting layer 21 are spirally coiled to form a fixed bed mechanism 11; the fixed bed mechanism 11 Among them, sampling strips 22 are arranged between adjacent first constituent layers 20 and second constituent layers 21, and be...

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Abstract

The invention discloses a detachable fixed bed bioreactor suitable for viral vector production, the detachable fixed bed bioreactor comprises a tank body, a culture bin, a stirring bin, a fixed bed mechanism and a sampling strip, the culture bin is mounted in the tank body, and the fixed bed mechanism is mounted in a culture bin accommodating part of the culture bin; the bottom of the culture bin is connected with the stirring bin, a spoiler is arranged between the culture bin and the stirring bin, and the spoiler is installed at the top of the stirring bin. When cell inoculation is carried out at the initial stage, thick cell suspension cannot be blocked at the bottom of the fixed bed, and cells can be uniformly distributed; in the cell growth process, culture medium liquid flow penetrates through the surface of the fixed bed mechanism at a constant speed and can make full contact with cells without dead corners, nutrient substances needed by the cells are more balanced, and the cell growth state is more uniform; the culture bin and the stirring bin can be conveniently mounted and dismounted, the interior of the fixed bed mechanism can be conveniently sampled, and the requirements of parameter testing and process development are met.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a detachable fixed-bed bioreactor suitable for the production of virus vectors, and belongs to the technical field of bioreactors. Background technique [0002] Cell therapy and gene therapy (CGT) can be used to upgrade the therapy or treat rare diseases at the molecular level through gene expression, silencing or in vitro modification. Viral vectors are currently the main means of delivery, and their expression and production are the core of CGT drug production. Most of the existing viral vector production processes are based on adherent cell platforms. Fixed bed bioreactor is a new type of cell culture device. Compared with traditional bioreactors, it can simplify the culture process of adherent cells and is easy to scale up. [0003] The internal structure of the existing fixed-bed bioreactor is complex and uneven, and it is easy to cause cell accumulation and blockage during cell inoculation...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/34C12M1/02C12M1/00
CPCC12M23/20C12M23/38C12M25/18C12M27/02C12M41/26C12M41/32
Inventor 谭虎王江凯韦博
Owner 同腾新创(苏州)科技有限公司
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