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Sample preparation method for shrimp meat sample non-targeted metabonomics and non-targeted lipidomics research

A metabolomics, non-targeting technology, applied in the field of analytical chemistry, can solve problems such as distribution in two phases or distribution to the interface, and achieve the effect of avoiding multi-platform data integration, saving instrument analysis time, and saving sample preparation time

Inactive Publication Date: 2021-03-19
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects and insufficiencies of the existing two-phase extraction methods, and provide an extract that can be directly obtained from the same sample by one-time extraction containing both hydrophilic and lipophilic compounds, and then can be extracted by a single instrument Analyze Extraction Methods for Simultaneous Study of Untargeted Metabolomics and Untargeted Lipidomi

Method used

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  • Sample preparation method for shrimp meat sample non-targeted metabonomics and non-targeted lipidomics research
  • Sample preparation method for shrimp meat sample non-targeted metabonomics and non-targeted lipidomics research
  • Sample preparation method for shrimp meat sample non-targeted metabonomics and non-targeted lipidomics research

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Example Embodiment

[0039]Embodiment 1

[0040]Include the following steps:

[0041](1) Sample pretreatment: go to the head, go to the end, after the shrimp wire treatment, allow it to pre-freeen overnight in the -80 ° C refrigerator, then cryogenically dry, low temperature grinding into powder;

[0042](2) Substation extraction: 3 pieces of powder shrimp samples of 120 mg pre-freeze, each addition of 1.2 ml of pre-free extract (methyl tert-butyl ether / methanol / water, 200: 100) : 35) and 12 steel beads, in the grinding instrument (50 hours of abrasive frequency, grinding time of 60s, ground, adjacent interval 30s);

[0043](3) Ultrasound extraction: After the grinding, the extraction mixture was subjected to water bath (30 ° C, 60 min), subsequently, centrifugal (4 ° C, 12000 rpm, 15min) was collected;

[0044](4) 3 parts of the above-mentioned supernatant, vortex oscillation, that is, the extract of the present invention.

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Abstract

The invention belongs to the technical field of analytical chemistry and discloses a sample method for shrimp meat sample non-targeted metabonomics and non-targeted lipidomics research, which comprises the following steps of putting a trace amount of freeze-dried shrimp meat sample into a pre-frozen extraction solution, homogenizing, carrying out water bath ultrasonic treatment, and centrifuging to obtain a supernatant for analysis. A solvent adding strategy of a solution is adopted, three solvents with large polarity differences are simultaneously used to form a three-solvent single-phase system, hydrophilic and lipophilic compounds are simultaneously extracted, and a necessary phase separation step in a two-phase extraction method is omitted, so steps of respective collection and analysis of two phases in the two-phase extraction method are not needed; the hydrophilic and lipophilic compounds are analyzed in the same extracting solution and in the same instrument analysis process. According to the method, the compound coverage is obviously improved, the sample flux is improved, a technical problem that part of compounds are simultaneously distributed in two phases and exist in aninterface in a two-phase extraction method is avoided, and the step of data integration of different analysis platforms is avoided.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and more particularly relates to a sample preparation method for non-targeted metabolomics and non-targeted lipidomics research on shrimp meat samples. Background technique [0002] Non-targeted metabolomics and non-targeted lipidomics have been widely used in research fields such as disease diagnosis, pathogenesis, treatment options, quality evaluation of traditional Chinese medicine, and food authenticity identification. Comprehensive coverage (extraction and analysis) of compounds is one of the goals of metabolomics and lipidomics. That is, when conducting untargeted metabolomics and untargeted lipidomics research, it is necessary to detect as many compounds as possible. Simultaneous untargeted metabolomics and untargeted lipidomics studies not only improve compound coverage, but given the association of hydrophilic compounds with lipophilic compounds, untargeted metabolomics and...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/38G01N1/42
CPCG01N1/28G01N1/286G01N1/38G01N1/42G01N2001/2866
Inventor 雷红涛钟鹏韦晓群李向梅沈兴徐振林孙远明
Owner SOUTH CHINA AGRI UNIV
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