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A kind of mass breeding method of pleated skirt Thai lion goldfish

A technology of Thai lion goldfish and pleated skirts, which is applied in the field of mass breeding of pleated skirt Thai lion goldfish, can solve the problems of the large season influence of Thai lion goldfish and the lack of breeding methods of Thai lion goldfish, so as to reduce background differences, develop Good, high single spawning results

Active Publication Date: 2021-11-19
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology still lacks an efficient method for breeding Thai lion goldfish, and the breeding of Thai lion goldfish is greatly affected by the season

Method used

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  • A kind of mass breeding method of pleated skirt Thai lion goldfish
  • A kind of mass breeding method of pleated skirt Thai lion goldfish
  • A kind of mass breeding method of pleated skirt Thai lion goldfish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for batch breeding of pleated skirt Thai lion goldfish, comprising the following steps:

[0035] S1. Seed cultivation: Raise freshly opened fry in a turnover box with a water temperature of 23-25°C and feed live Artemia 3 times a day. The turnover box is 60-100cm long, 40-60cm wide, 20-30cm high, and the water level is 15 -20cm is appropriate. Install a sponge filter on one side of the turnover box to filter, and place an oxygen increasing head next to it for oxygenation, and the air volume is appropriate. Clean the sponge filter once a week.

[0036] After the fry exceed 2cm in length, look down to select four-tailed fry, and then look sideways to remove the deformed and folded fry, and put the selected high-quality fry into a new container for rearing, with a density of about 2 fish per liter of water; the container is equipped with The 7-watt small water pump adds water by trickle, and the daily water intake is about 1 / 5 of the total water body. The three-...

Embodiment 2

[0047] Example 2: Creation of Dragon Eye Pleated Skirt Thai Lion Goldfish

[0048] On the basis of Example 1, in the artificial insemination step, gene editing can be performed on the collected fish eggs to regulate and obtain the pleated skirt Thai lion goldfish with corresponding traits expressed, the steps are as follows:

[0049] S1. Target design: Sequence the gene of the pleated skirt Thai lion goldfish to obtain the DNA sequence and amino acid sequence of the gene that regulates the traits of the dragon eye, and use the wildcard GG[G / A]? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? GG or CC? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? [T / C] CC designs a suitable knockout target on the exon of the DNA sequence of the gene that regulates the traits of dragon eyes, builds a local genome database after obtaining the target, and uses blast software to search and verify the uniqueness of the target After determining the uniqueness, the target sequence will be tr...

Embodiment 3

[0063] Example 3: Creation of Dragon Eye Pleated Skirt Thai Lion Goldfish

[0064] The creation method of this embodiment 3 is the same as that of embodiment 2, the difference is that in step S5, the fertilized eggs are subjected to heat shock treatment, and the specific operations of S5 are:

[0065] S5. Gynogenesis: take carp sperm to inactivate, mix inactivated sperm with F0 generation female pleated skirt Thai lion goldfish eggs, and then perform heat shock treatment. The heat shock treatment method is to develop fertilized eggs at a constant temperature of 24°C for 43 After -47min, it was placed in ice water at 41°C for 2min, and the fertilized eggs after treatment hatched to be F1 generation homozygotes with dragon eye traits.

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Abstract

The invention relates to fish breeding technology, in particular to a batch breeding method of pleated skirt Thai lion goldfish. The method includes the steps of seed cultivation, parent cultivation, artificial insemination and gynogenesis. The present invention establishes a high-efficiency breeding method for Thai lion goldfish, realizes continuous spawning of goldfish, and is not subject to seasonal control, provides a stable source of fish eggs for experimental research relying on fish eggs, and through the establishment of optimal breeding methods, During the process from the hatching of the fertilized eggs until the sexual maturity of the individual fish, the high hatching rate of the fertilized eggs and the low mortality rate of the fry are achieved. The adult fish are well developed, healthy and vigorous, providing the market with a high-quality source of ornamental goldfish.

Description

technical field [0001] The invention relates to fish breeding technology, in particular to a batch breeding method of pleated skirt Thai lion goldfish. [0002] technical background [0003] Goldfish originated from the tetraploid crucian carp in my country. It is an ornamental fish formed after hundreds of years of artificial selection, and it can be called the quintessence of our country. Traditional goldfish are kept in fish pots, and the breeding of species is mainly based on looking down. The popularity of fish tanks has made side viewing a trend. The pleated skirt Thai lion is one of the most important ornamental species of side-view goldfish. Its body color is changeable and its swimming posture is beautiful. It is deeply loved by goldfish players and has a broad market. However, the prior art still lacks an efficient breeding method for Thai lion goldfish, and the breeding of Thai lion goldfish is greatly affected by seasons. It is of great significance how to esta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10C12N15/89C12N9/22C12N15/12
CPCA01K61/10C07K14/775C12N9/22C12N15/89Y02A40/81
Inventor 余鹏桂建芳周莉汪洋李志李亮亮杨小丽
Owner INST OF AQUATIC LIFE ACAD SINICA
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