66 results about "Gold fish" patented technology

The invention discloses an enzyme-linked immunosorbent kit for detecting the vitellogenin of goldfish, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the vitellogenin of the goldfish is prepared; secondly, the polyclonal antibody of rabbit anti-vitellogenin of the goldfish is prepared; thirdly, the polyclonal antibody of the rat anti-vitellogenin of the goldfish is prepared; and fourthly, respectively one bottle of the pure product of the vitellogenin of the goldfish, the polyclonal antibody of the rabbit anti-vitellogenin of the goldfish and the polyclonal antibody of the rat anti-vitellogenin of the goldfish, one block of blank 96-hole enzyme-linked immunosorbent assay board and respectively one bottle of the serum of a negative contrasting group, coating liquid, confining liquid, sample diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by horse radish peroxidase are filled into a box body together, thus obtaining the enzyme-linked immunosorbent kit for detecting the vitellogenin of the goldfish. The kit of the invention can be applied to the survey that the internal secretion disturbs chemical substances and can quantitatively detect the vitellogenin in the blood of the goldfish, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.

The present invention relates to aquarium fish raising technology and is the raising method and special apparatus of raising aquarium fish. Unlike the background technology, which has aquarium fish capable of swimming only in aquarium, the present invention has some increased special apparatus for the aquarium fish to form certain fish stream with increased enjoying effect.

The invention relates to a grass goldfish pelvic fin cell line and a constructing method thereof. Tissue blocks are digested through trypsin, EDTA and hyaluronidase according to the features of grass goldfish pelvic fin tissue histocyte, the digested tissue blocks are transplanted to a cultivation bottle, special multiplication cultivation solution is added to the cultivation bottle, primary culture is conducted, and then the grass goldfish pelvic fin cell line is successfully constructed. By means of the method, a large number of cells can be separated three days after primary culture is conducted, and the cells have the continuous passage capability. After passage is stable, the cells are in a typical fiber form, it proves that the Chinese grass goldfish pelvic fin cell line is stable in character and uniform in component and is a good material for studying the aquatic animal virology and the immunology. At present, the grass goldfish pelvic fin cell line has 60 generations, and the cells can stably increase and have the continuous passage capability.

The invention discloses a goldfish autonomous transposon gene gfTol2, which has a nucleotide sequence shown as SEQ ID No:1. The invention also discloses a transposase coded by the gene gfTol2, which has an amino acid sequence shown as SEQ ID No:2. The invention also discloses use of the gene gfTol2 as a gene medicament or a gene reagent for goldfish; and the rate of graded product of the goldfish is improved and offspring mutation is induced by adjusting the concentration of a transcription product of the gene.

The invention relates to a test kit for qualitatively detecting fish vitellogenin by utilizing lipovitellin antibody. The test kit comprises pure product of goldfish lipovitellin and anti-rat goldfish lipovitellin polyclonal antibody. The preparation method of the test kit comprises the following steps: obtaining goldfish lipovitellin through purifying at first; then preparing anti-rat goldfish lipovitellin polyclonal antiserum through immune rats; and further purifying anti-rat goldfish lipovitellin polyclonal antibody. According to the invention, the test kit can be used for screening environmental estrogen materials and monitoring the situation of water environment which is polluted by the environmental estrogen materials, so that vitellogenin in fish blood, overall uniform serous fluid, skin mucus, liver tissues and hepatic cell nutrient solution can be monitored sensitively and conveniently. The test kit can be used for qualitatively detecting cyprinidae fish vitellogenin, and has the same monitoring sensitivity to the fish vitellogenin of a far provenance relationship fish. The biggest advantage of the test kit is that the test kit has a very wide application scope, and the minimum monitoring limit can reach 100ng / ml.

The invention discloses a gold fish stick and a manufacturing method thereof. The gold fish stick comprises components by weight percentage of 20%-40% of fresh-water fish, 3%-15% of marine minced fillet, 1%-3% of pre-wrapping powder, 1%-5% of wrapping slurry powder, 1%-3% of modified starch, 5%-16.5% of breadcrumb, 0.2%-1% of fish essence, 0.3%-2.5% of composite phosphate and the rest of auxiliary materials. According to the gold fish stick, the fresh-water fish is utilized as a main raw material to manufacture powder-wrapped fried food, so that the cost is greatly reduced. By optimizing the best raw material and matching, the gold fish stick which has good taste, flavor and appearance, has good refrigeration resistance and can be stored for a long time can be obtained.

The invention relates to a method for detecting the insertion flanking sequence and copying quantity of a Tgf2 transposon in a goldfish genome. The method comprises the following steps of: extracting the DNA (Deoxyribose Nucleic Acid) of a goldfish genome with a Tgf2 transposon; performing restrictive incision enzyme digestion on the DNA of the genome; connecting an enzyme-digested DNA fragment with a splinkerette joint; and performing primary PCR (Polymerase Chain Reaction) and secondary PCR respectively to amplify flanking sequences of transposon insertion loci 5' and 3' respectively; and performing cloning and sequence measurement on a product of the secondary PCR to detect the flanking sequence of the transposon in a fish genome and presume an inserted copying quantity. The method has the advantages of easiness, convenience, high efficiency and low cost, and a rapid and accurate method can be provided for the detection of the copying quantities and flanking sequences of other fish genome insertion mutation and transgenic descendants mediated by the Tgf2 transposon.

The invention belongs to the field of aquatic product disease prevention and treatment, and relates to a method for building a gyrodactylus purebred artificial infection system, in particular to a method for building an artificial infection system of gyrodactylus kobayashii hukuda purebred parasitized on the gill and the body surface of goldfish. The method includes the steps of (1) obtaining healthy and gyrodactylus-free goldfish; (2) conducting gyrodactylus artificial infections on the healthy and gyrodactylus-free goldfish; (3) maintaining the state after the gyrodactylus artificial infections are carried out. By means of the method for building the gyrodactylus purebred artificial infection system, an animal model is provided for screening medicine, developing new fish medicine and the like with the goldfish as hosts.

The invention discloses a cultivation method for a hybridized goldfish. The cultivation method comprises the following steps: S1, taking bred red-eye white butterfly-type tail goldfishes as female parents and taking bred green long-tail ryukin goldfishes as male parents; hybridizing the two types of the goldfishes to obtain a first-filial generation F1; S2, selecting five-color butterfly-type tail ryukin goldfishes and ink-color butterfly-type tail ryukin goldfishes to be hybridized to obtain a second-filial generation F2; S3, selecting female and male three-color butterfly-type tail ryukin goldfishes from the second-filial generation F2, and carrying out self-hybridization on the female and male three-color butterfly-type tail ryukin goldfishes to obtain three-color butterfly-type tail ryukin goldfish breeds capable of stably inheriting three-color butterfly-type tail ryukin properties. According to the cultivation method, the selection and the hybridization of the goldfish breeds are carried out to cultivate the new goldfish breeds, so that the variety of the goldfishes is enriched.

The invention relates to the fields of cosmetic products and in particular relates to a golden hydrotherapy composition and a using method thereof. The golden hydrotherapy composition is prepared from the following components in parts by weight: 52-55 parts of wisdom golden water, 12-14 parts of gold introduction liquid, 6-7 parts of gold stock solution, 6-7 parts of thin skin growth promoting liquid, 6-7 parts of hyaluronic acid stock solution, 6-7 parts of black gold fish egg essence and 6-7 parts of snail protein stock solution. Compared with the prior art, the golden hydrotherapy composition has remarkable effects of fading and removing spots and is also capable of obviously increasing water content of skin and brightening whole skin color of a face.

The invention relates to a method for preparing a transparent skeleton specimen of a small animal, and a specimen prepared with the method. According to the invention, muscle of the small animal become transparent, and a colouring agent is adopted for soaking to develop and distinguish different parts of an animal body, so that original position structures of hard bones and cartilages of the animal body are showed directly and accurately. The method is simple in step, the adopted agent is safe and can be used for preparing transparent skeleton specimens of small animals such as goldfishes, tropical fishes, frogs and mice, the prepared specimens can be kept for a long time, structures are showed clearly, and the prepared specimens have relatively high scientific research and teaching values.

The invention provides a kit for detecting goldfish haematopoietic necrosis virus. The invention provides a real-time fluorescence quantitative PCR (Polymerase Chain Reaction) primer and a probe for detecting goldfish haematopoietic necrosis virus at first, wherein the sequences are respectively represented by SEQ ID NO2, SEQ ID NO3 and SEQ ID NO4. The invention further provides a method for detecting goldfish haematopoietic necrosis virus by using the primer and the probe through fluorescence quantitative PCR. The detection method and the detection kit disclosed by the invention have the advantages of being accurate to detect, high in sensitivity and specificity, simple, convenient and rapid, and have good clinical sample detection capability.

In order to better protect growth of goldfishes and realizes automatic water changing and automatic feeding when there is no person, a simple auxiliary fish-culturing device is designed by utilizing principles such as gravity, lever and pulley to solve the water changing and fish-feeding problems when a person leaves the room for a long time.

The invention relates to a fish molecular design breeding technology, in particular to a goldfish gene editing technology and a method for creating a new goldfish variety by using the gene editing technology. The method comprises steps of knockout target design, microinjection, fertilization incubation, gynogenesis and high-temperature sex reversal. According to the invention, a CRISPR / Cas9 gene editing technology is established on goldfish, so that a target phenotype is generated on F0 generation goldfish, an efficient gynogenesis condition of pleated Thai lion goldfish is determined through experiments, homozygotes are obtained in F1 generation, and a large number of groups are expanded in F2 generation through temperature-induced reversion. Through a molecular breeding means, transfer of a single target character is rapidly achieved, the goldfish gene editing technology and the method can be used for creating a new goldfish variety, provides technical support for molecular design and breeding of goldfish, and is beneficial to promote development of goldfish molecular breeding technology.

The invention provides a river crab and goldfish intercropping ecological efficient breeding method. The breeding method comprises the following steps: a culture area is selected as a culture pond; aquatic plants are planted in the culture pond in the first year of culture; in the early spring period, goldfish fry and crab fry are put into the culture pond; and after the crabs and the goldfishes are mature, the crabs and the goldfishes are sold on the market. According to the breeding method, crabs and four-tail goldfishes are bred together, organic matter at the bottom of the culture pond is subjected to aerobic decomposition through microbial thalli on the basis of bait aquatic plants and algae of the crabs, the number of insects in the water body is reduced by breeding the goldfishes, a biological chain cycle is formed in the water body, by referring to a natural biological ecological chain, the stability of the biological chain is artificially maintained, and the purpose of ecological breeding is achieved.

The invention discloses application of a rosemarinus officinalis extract in preparation of a medicine for preventing and controlling ichthyophthirius multifiliis fouquet, and belongs to the technical field of prevention and control of fishery diseases. According to the invention, the rosemarinus officinalis extract is applied to prevention and control of ichthyophthirius multifiliis fouquet for the first time, and 100% of killing rate of ichthyophthirius multifiliis fouquet can be achieved in an effective dose range. The median lethal concentration of the rosemarinus officinalis extract to goldfish respectively is 28.6 times and 9.4 times of the median effective concentration of the rosemarinus officinalis extract for killing ichthyophthirius multifiliis theront and trophozoite, and the rosemarinus officinalis extract is safe to fish within the effective range of killing insects. Rosemarinus officinalis resources are rich, the preparation process of the rosemarinus officinalis extract is simple, and the rosemarinus officinalis extract has the potential of being developed into a novel green alternative drug for preventing and controlling ichthyophthirius multifiliis fouquet.

The invention provides a goldfish breeding feed and a preparation method thereof. The goldfish breeding feed is prepared from the following ingredients: soybean meal, peanut meal, earthworm meal, fishmeal, carrots, distiller's grains, zeolite powder, betaine, allicin, onion powder, seaweed powder, Chinese prickly ash oil, an enzyme preparation, organic acid, an adhesive, a nutritional agent and water. The goldfish breeding feed prepared by the preparation method is capable of supplementing sufficient proteins, carbohydrates and fat so as to meet nutrient demands of goldfishes at different growth stages, as well as improve survival rate of goldfishes; moreover, the goldfish breeding feed is rationally formulated, balanced and comprehensive in nutrients, simple in preparation process, low in preparation cost, easy to obtain raw materials and worth popularization.

The invention relates to a method for detecting the insertion flanking sequence and copying quantity of a Tgf2 transposon in a goldfish genome. The method comprises the following steps of: extracting the DNA (Deoxyribose Nucleic Acid) of a goldfish genome with a Tgf2 transposon; performing restrictive incision enzyme digestion on the DNA of the genome; connecting an enzyme-digested DNA fragment with a splinkerette joint; and performing primary PCR (Polymerase Chain Reaction) and secondary PCR respectively to amplify flanking sequences of transposon insertion loci 5' and 3' respectively; and performing cloning and sequence measurement on a product of the secondary PCR to detect the flanking sequence of the transposon in a fish genome and presume an inserted copying quantity. The method has the advantages of easiness, convenience, high efficiency and low cost, and a rapid and accurate method can be provided for the detection of the copying quantities and flanking sequences of other fish genome insertion mutation and transgenic descendants mediated by the Tgf2 transposon.

An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1(DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22° C. in shaking of 150-200 rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.

The invention discloses a feed formula for goldfish in a growth period and a preparation method thereof and belongs to the fields of agriculture farming and goldfish breeding. The feed formula comprises fish meal, soybean meal, peanut meal, rapeseed meal, flour, bran, monocalcium phosphate, astaxanthin, betaine, soybean lecithin, fish oil, soybean oil, vitamin and microelement premix. The method comprises the following steps of (1) crushing fish meal, soybean meal, peanut meal, rapeseed meal and bran through a 60-mesh screen, weighing the crushed raw materials and then evenly mixing the crushed raw materials with betaine and astaxanthin at a ratio; (2) weighing soybean lecithin, fish oil and soybean oil, and stirring and mixing the soybean lecithin, the fish oil and the soybean oil evenly;and (3) mixing a mixture obtained in the step (1) with the mixture obtained in the step (2) evenly, and preparing a feed with the particle size of 2.0 mm by a granulator. The prepared feed nutritioncomposition for the goldfish in the growth period has the effects of being abundant in nutrition, good in palatability, gorgeous in body color and high in disease resistance; and the death rate of thegoldfish fed with the prepared feed is significantly reduced in comparison with that fed with a commercial common feed.

A water quality regulator for grass goldfish breeding is prepared from the following raw materials (by weight): 10-15 parts of a microbial agent, 5-10 parts of lotus leaf powder, 10-15 parts of foliumartemisiae argyi powder, 30-40 parts of an adsorbent, 10-15 parts of rhizoma coptidis powder, 5-10 parts of radix isatidis powder, 4-8 parts of cortex cinnamomi powder, 5-8 parts of pectinase and 8-12 parts of sodium bicarbonate. Through mutual cooperation of all the substances, the water quality is jointly adjusted from multiple aspects of adsorption flocculation, toxic substance decomposition,water body microbial community improvement, water body pH adjustment and water body clarification, secondary toxic substances and secondary pollution will not be generated, and a proper water qualityenvironment is provided for the growth of grass goldfish.

The invention discloses a disinfectant for disinfecting body surfaces of goldfishes and belongs to the technical field of fish medicines. The disinfectant is prepared by the following method comprising the following steps: 1) weighing 30%-40% of fructus cnidii and 60%-70% of melia azedarach leaves according to the weight percent content, adding water which is 3-4 times weight of the total medicinal materials, soaking for 2-3 hours, then decocting at 100 DEG C for 30-40 minutes, finally filtering and removing dregs, and collecting filtrate to obtain traditional Chinese medicine liquid for later use; and (2) weighing 5%-7% of chitin, 2%-12% of gallic acid, 2%-10% of furacilin and 72%-90% of the traditional Chinese medicine liquid according to the weight percent content, mixing and dissolving evenly, so as to obtain the disinfectant. The disinfectant for disinfecting the body surfaces of goldfishes is good in prevention effect, is capable of eliminating bacteria on the body surfaces of goldfish and improving the immunity of the fishes, and has a good treatment effect on goldfish white spot, gill rot disease, a loose scale disease and saprolegniasis; the drug resistance to single antibiotic is overcome; and meanwhile, a positive effect can be generated for prevention of recurrence or infection of fish diseases.

The invention relates to a fish molecular design breeding technology, in particular to a method for creating jade rabbit pleated skirt Taishi goldfish. The method comprises the steps of target spot design, microinjection and fertilization incubation. On the basis of a CRISPR / Cas9 gene editing technology, after a pigment gene oca2 is knocked out, jade rabbit goldfish with red eye characteristics is generated on F0-generation goldfishes, efficient gynogenesis conditions and high-temperature group expansion conditions of pleated skirt Taishi goldfish are determined through experiments, and jade rabbit pleated skirt Taishi goldfish homozygotes are obtained on F1-generation goldfish; and through temperature-induced reversion, the F2 generation is massively expanded. By means of molecular breeding, transfer of the single-purpose character is rapidly achieved, the jade rabbit pleated skirt Taishi goldfish which is the variety with the high ornamental value is stably and rapidly created, and wide application prospects and market values are achieved.

Although an ornamental fish tank arranged in a modern home increases a lot of fun and color for family taste, the ornamental fish tank is high in manufacturing cost, and rare fishes such as goldfish and swallow fish are high in price and extremely difficult to manage and breed. For some rare endangered animals or dangerous animals, the close and free observation of the animals and no disturbance of the animals are problems to be solved. The invention discloses an electronic real scene viewing system which uses a high-definition camera or a camera to continuously shoot real scenes at multiple angles, the shot real scenes can be separated by one-way light-transmitting objects, a transparent glass room or a fish tank is formed by a plurality of screens through a receiving and displaying interaction unit in a viewing place, the synchronous and real scenes can be seen, and goldfish can be fed by pressing a button; a giant aquarium and a beast garden can be built in the venue, habits and food intake of dangerous organisms in a real scene can be viewed in a near-front manner, field interaction facilities can be controlled to interact, which is splendid, excited, sanitary and safe, and feelings similar to those in the field can be enjoyed.

The invention relates to a high-energy feed for safe overwintering of goldfish. The high-energy feed is characterized by comprising the following components in percentage by weight: 0.1%-0.3% of trace elements, 0.03%-0.06% of vitamins, 10%-15% of corn, 5%-10% of soybean meal, 5%-10% of fish meal, 5%-10% of shrimp powder, 10%-20% of miscellaneous meals, 3%-5% of soybean oil, 0.1%-0.3% of choline chloride and 20%-40% of flour. The high-energy feed is used in the later period of goldfish breeding. High-quality raw materials are selected to ensure nutritional requirements of fish bodies; dietary fibers are added to improve intestinal canal environments; the choline chloride is added to promote fat metabolism and protect liver and gallbladder; the corn and the soybean oil are added to increase the energy of the feed and ensure the condition factor of the goldfish, so that the goldfish has enough energy to overwinter; and the vitamin is added so that the goldfish bodies are relatively healthy, the stress bleeding during selling is reduced, and the goldfish can safely overwinter.

The invention discloses a newly bought goldfish feeding water-passing intelligent motor device. The newly bought goldfish feeding water-passing intelligent motor device comprises a machine body, wherein a main space is formed in the machine body, a small space is formed on the lower side of the main space, a pause space is arranged on the lower side of the small space, and a pause device is arranged in the pause space. Newly bought goldfishes can automatically pass water, so that the goldfishes are prevented from directly entering a new environment of a fish tank and getting sick or dying due to the fact that the goldfishes do not adapt to the suddenly and violently changed environment. Two processes of temperature balance and water environment change balance can be controlled through a motor controlled through temperature. A motor controlled through continuous pressure is used for monitoring the possible inadaptation phenomenon of the goldfishes in the water passing process in real time, water passing is temporarily stopped to be buffered by the goldfishes, and it is guaranteed that water passing is normally completed.

The invention relates to red and white flower goldfish bait, which is prepared from vivipara meal, shell meal, tenebrio molitor meal, loach meal, bone meal, vegetable protein powder, clam worm meal, sorghum gluten powder, strawberry powder, dasheen powder, cassia seed powder, dandelion powder, yam flour, saffron powder and bulked corn powder. All kinds of powder are mixed together according to a ratio, the mixed powder is fed in a mixer to be mixed for 180 minutes, the mixture is taken out and fed in a grinder to be ground into 0 mesh powder, the powder is taken out and fed into a dryer, the dryness of the dried material is controlled to be 90 to 95 percent, the dried powder is taken out, cooled and fed into the mixer again to be mixed for 180 minutes, the mixed powder is taken out and fed into a bulking machine to be bulked, the temperature of the bulking machine is controlled to be between 80 and 85 DEG C, the bulking time is 2 to 3 hours, the dryness of the bulked material is controlled to be 90 to 95 percent, and the powder is taken out, cooled and packaged by specific weight.

The invention relates to the technical field of aquaculture, in particular to a household indoor goldfish culture method. The goldfish culture method comprises the following steps: injecting water into a fish tank, and illuminating and ventilating the fish tank to culture green algae and green moss in a water body; when green mosses appear on the tank wall of the fish tank or green algae appear in the water body, the original water in the fish tank is completely discharged, then new water is injected, the water is aerated, and then the goldfishes are put, and the putting density is that the putting number of the goldfishes with the length of 12-15 cm in each cubic meter of the water body is smaller than 18; the goldfish is fed with baits, the baits comprise granular baits and frozen blood worms, the granular baits and the frozen blood worms are fed at intervals, and after the granular baits are continuously fed for two days, the frozen blood worms are fed for one day; adjusting the water quality of the water body in the fish tank, namely extracting fish excrement at the bottom every day and supplementing the same amount of fresh water to the original water height.

The invention discloses a Chinese herbal medicine for treating lepidorthosis of gold fish. The Chinese herbal medicine is prepared from the following raw materials: 1-3 parts of Chinese rhubarb, 1-2 parts of rhizoma coptidis, 2-3 parts of poria cocos, 2-3 parts of juncus effusus, 2-4 parts of dried ginger, 1-5 parts of calamitas urinae hominis, 1-4 parts of reed rhizome, 2-5 parts of radix ophiopogonis, 1-4 parts of radix isatidis, 1-4 parts of herba artemisiae scopariae, 1-3 parts of dandelion, 1-3 parts of immature bitter orange, and 2-5 parts of honeysuckle stem. The Chinese herbal medicine is prepared from the following steps: cleaning the raw materials, adding an appropriate amount of water to the cleaned raw materials, and decocting the cleaned raw materials and the water for 30-40 minutes to obtain the filtrate. The Chinese herbal medicine for treating lepidorthosis of gold fish adopts a pure biological preparation, the lepidorthosis can be effectively cured, after the lepidorthosis is cured, the Chinese herbal medicine has a repairing effect on scales, the color and the lustre of the repaired scales are similar to those of the scales without the lepidorthosis, and the viewing effect cannot be influenced.

The invention relates to a separating goldfish cultivating device which comprises at least one box body arranged in a pool in a floating manner, wherein each box body consists of a bottom surface and side surfaces connected end to end, the lower ends of the side surfaces are sealed with the bottom surface, the bottom surface or the side surface of each box body are provided with hollowed holes for communicating the inside of the box body from the pool, the bottom area of each box body is less than 10m<2> and the depth of the bottom surface immersed into the water level is 20-30cm, and goldfishes are cultivated in each box body. The hollowed box bodies are used for cultivating the goldfishes in the pool to ensure that the quality of water for cultivating the goldfishes is better, and the goldfishes can not get sick or even dead due to poor quality; water does not to be replaced every day, thus a lot of workloads are saved; and body types of the goldfishes are more attractive due to thesmall-volume box bodies.