71 results about "Gold fish" patented technology

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The utility model discloses a goldfish culture system, which relates to the goldfish culture technical field, and comprises a wooden sea, the bottom of the wooden sea is provided with a support, the top opening is fixedly sleeved with a fixing ring, the bottom of the fixing ring is slidably connected with a cleaning assembly used for cleaning the inner wall of the wooden sea, and the top of the fixing ring is fixedly provided with a filtering assembly. A spraying head is fixedly arranged at the top of the filtering assembly through a connecting frame, a water pipe is arranged in the connecting frame, one end of the water pipe is communicated with the spraying head, and the other end of the water pipe extends into the wooden sea. The filtering assembly is arranged, water in the wood sea is filtered in a circulating mode, the water changing frequency is effectively reduced, the inner wall of the wood sea can be automatically cleaned through the cleaning assembly, and the labor intensity of workers is reduced.

The invention provides a feed for increasing dark red body color of goldfish and a preparation method of the feed, and relates to the field of fish feeds. The feed comprises the following raw materials in percentage by mass: 1-20% of fish meal, 5-20% of euphausia superba powder, 5-30% of corn flour, 2-20% of chrysanthemum powder, 10-30% of flour, 10-20% of vital gluten, 1-5% of bee pollen, 1-10% of carrot powder, 3-20% of chili meal, 0.1-3% of astaxanthin, 1-10% of spirulina, 1-10% of haematococcus pluvialis, 1-10% of phaffia rubra, 1-8% of fish oil and 0.1-3% of choline chloride. 0.01-3% of composite vitamins and 0.01-5% of composite minerals. After the feed is fed for a long time, the red brilliance and the brightness of the body color of the dark red goldfish can be improved, and the dark red goldfish can present a more beautiful swimming posture and a ruddy color.

The invention discloses an application of alkannin in resisting fish ichthyophthiriasis. The alkannin has remarkable killing activity on ichthyophthirius multifilis in an in-vitro stage, the 4-hour half effective insecticidal concentration (4 h-EC50) of the alkannin on a grazing body is 0.048 mg / L, and the 22 h-EC50 of the alkannin on a cyst is 1.275 mg / L. Besides, the shikonin has a 96-hour median lethal concentration (96h-LC50) of 2.229 mg / L to goldfish and a treatment index (TI) of 46.4 to a grazing body, and can be safely applied to fishes in an effective insecticidal concentration range. The shikonin disclosed by the invention has the characteristics of high insecticidal activity and low toxicity to fishes, has the potential of being developed into novel green fish ichthyophthiriasis-resistant aquatic products such as fish medicines, feed additives and the like, and has a wide application prospect.

1. The name of the design product: olive kernel carving (goldfish). 2. The use of the design product: used for kernel carving handicraft, used for playing, decoration, handicraft ornament, etc. 3. The design points of the design product: in shape. 4. The picture or photo that can best show the design points: perspective view.

The application discloses a method for preparing goldfish feed from fermented abalone viscera powder, and comprises the following steps: preparing fermented abalone viscera powder, including raw material pretreatment, strain activation, anaerobic fermentation and final treatment; preparing a formula of the goldfish feed, including fermented abalone viscera powder, spirulina powder, fish meal, soybean meal, rice bran, wheat flour, fish oil, alpha-starch, soybean lecithin, calcium dihydrogen phosphate, vitamin premix, mineral premix, betaine, garlicin, VC-phosphate, lysine and choline chloride; and preparing the goldfish feed, including accurate batching, uniform mixing, moisture adjustment and molding, grading granulation, drying and cooling and grading screening. The fermentation and feed preparation process is simplified, the complicated enzymolysis-fermentation combined process is abandoned, no professional technical personnel are needed, and the household breeders can prepare the feed through simple equipment, and the enterprises can mass-produce the feed; the formula is optimized for goldfishes in different growth stages, is suitable for multiple goldfishes such as golden goldfish and grass goldfish, and has high application flexibility.

1. Name of the designed product: shower holder (goldfish). 2. Use of the designed product: fixing shower. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view 1.

By implementing measures to restrict the robot's range of movement on the game board (2) at the competition venue, it will be possible to participate in the competition from the internet, just as it is to participate in the competition at the venue. [Solution] To achieve this, the robot will be equipped with a movement range recognition system that ensures it can only move within its designated range, preventing interference with robot operation, and allowing it to be controlled similarly both at the competition venue and over the internet. Furthermore, the number of goldfish scooped will be counted fairly.

The utility model discloses a goldfish culture tail water treatment device, which relates to the technical field of tail water treatment, and adopts the technical scheme that the goldfish culture tail water treatment device comprises a pool body, a floating plate is arranged in the pool body, a plurality of through holes which are uniformly distributed in a matrix shape are formed in the floating plate, and ropes are fixedly arranged on two sides of the floating plate; the purifying device is located on the front side of the pond body, pump bodies are arranged on the two sides of the purifying device, and first pipelines are fixedly connected between the two pump bodies and the pond body. The goldfish breeding tail water purifying device has the advantages that the pipelines, the two pump bodies and the third pipeline are designed, goldfish breeding tail water can enter the purifying device through the second pipeline, then the tail water is treated, and the tail water is purified. After the tail water is treated, the tail water is discharged into the pond body, so that the floating plate in the pond body can float on the water, a user can plant aquatic vegetables and other aquatic crops on the floating plate, the tail water is not directly discharged after being treated by the treatment device, and the water is reutilized; therefore, the utilization rate of water resources can be increased.

The application discloses a goldfish molecular marker assisted breeding method, relates to the ornamental fish genetic breeding technical field, and aims at overcoming the defects of inbred degeneration and uncontrollable germplasm in new strain breeding. The method comprises the following steps: selecting a wishful eye goldfish with wishful eye traits and carrying first and second molecular markers in homozygosis as a first parent, and selecting a skin ball pearl goldfish with pearl eye traits as a second parent; performing the first parent and the second parent with normal and reverse crossing to construct two hybrid families; performing brother-sister intercross of F1 generation of the two families; screening double marker homozygous individuals; screening a candidate wishful pearl strain with wishful eye and pearl eye traits; and performing interfamily hybridization of the candidate strains of the two families to obtain a target goldfish new strain. The application can overcome the germplasm degeneration caused by inbreeding, and obtain a new goldfish strain with visible pupil and round and beautiful body shape when looking down, i.e., the wishful pearl, and realize germplasm tracking and intellectual property protection through molecular markers.

The utility model relates to the technical field of aquariums, and provides an indoor goldfish culture aquarium which comprises a water supply system and a water storage tank communicated with the water supply system. The first fish culture tank, the second fish culture tank and the third fish culture tank are sequentially arranged in a stepped manner from top to bottom; the water inlet system communicates with the water storage tank; the drainage system is communicated with the first fish culture tank, the second fish culture tank and the third fish culture tank; the aeration sewage suction device is respectively connected with the first fish culture tank, the second fish culture tank and the third fish culture tank; and the illumination device is arranged below the water storage tank. According to the indoor goldfish culture aquarium, due to the fact that the first fish culture tank, the second fish culture tank and the third fish culture tank are arranged in a trapezoid mode, three-dimensional space can be effectively utilized, and goldfishes can be conveniently overlooked and viewed; a still water environment can be created through the aeration sewage suction device, and cultivation and viewing of the goldfish are facilitated; the defect of insufficient indoor illumination is overcome by utilizing the illumination device; the water storage tank and the water inlet and outlet system are designed, so that water inlet and outlet do not consume electric energy.

The utility model discloses a net cage for pond culture goldfish, which relates to the technical field of goldfish culture and comprises a frame, a netting, a net cover and a load-bearing oxygenation mechanism, the frame is formed by splicing PVC (polyvinyl chloride) pipes and is of a cuboid structure, the netting is arranged on the surface of the frame to form the net cage, the net cover covers the top of the net cage, and the load-bearing oxygenation mechanism is arranged on the net cover. A hanging ring is arranged at the corner of the top of the net cage, an air inlet pipe is arranged in the PVC pipe, a pipe opening of the air inlet pipe penetrates out of the bottom of the vertical PVC pipe, the two ends of the load-bearing oxygenation mechanism are arranged on the air inlet pipe through pipe hoops, and the load-bearing oxygenation mechanism is fixed above the PVC pipe at the bottom of the frame through an 8-shaped buckle. According to the net cage, oxygen can be continuously supplied through the load-bearing oxygenation mechanism, water quality is kept clean, the breathing requirement of goldfishes is met, and the health problem caused by oxygen deficit of the goldfishes is avoided.

The application discloses a goldfish molecular marker assisted breeding method, relates to the ornamental fish genetic breeding technical field, and aims at overcoming the defects of inbred degeneration and uncontrollable germplasm in new strain breeding. The method comprises the following steps: selecting a wishful eye goldfish with wishful eye traits and carrying first and second molecular markers in homozygosis as a first parent, and selecting a skin ball pearl goldfish with pearl eye traits as a second parent; performing the first parent and the second parent with normal and reverse crossing to construct two hybrid families; performing brother-sister intercross of F1 generation of the two families; screening double marker homozygous individuals; screening a candidate wishful pearl strain with wishful eye and pearl eye traits; and performing interfamily hybridization of the candidate strains of the two families to obtain a target goldfish new strain. The application can overcome the germplasm degeneration caused by inbreeding, and obtain a new goldfish strain with visible pupil and round and beautiful body shape when looking down, i.e., the wishful pearl, and realize germplasm tracking and intellectual property protection through molecular markers.

The utility model discloses a goldfish cultivation greenhouse relates to the technical field of the greenhouse, and its technical scheme is: including the shed framework, the shed cloth is covered outside the shed framework, and the shed cloth one side is equipped with covering mechanism, covering mechanism includes two support frames, and two support frames are equipped respectively in the both sides of shed framework, and the outer surface of support frame is provided with the sliding slot, and covering mechanism includes unwinding subassembly and winding subassembly, and unwinding subassembly includes two first side plates, and two first side plates are fixedly connected with two support frames respectively, and the transmission shaft is connected through bearing between two first side plates, and one first side plate one side is equipped with the first motor fixedly, and the first motor output is fixedly connected with the transmission shaft, and the transmission shaft is coiled with the shade cloth outside, and the shade cloth one side is equipped with the connecting rod fixedly, and the beneficial effect is: effectively adjust the light and temperature condition in the greenhouse, provide a more stable, suitable growth environment for goldfish, and the operation efficiency is improved greatly through the manual regulation side shade curtain cloth quick release.

The utility model relates to aquatic products breeding equipment technical field provides a kind of goldfish artificial propagation post-rehabilitation maintenance device.The goldfish artificial propagation post-rehabilitation maintenance device includes box, drives component and partition component.The inside of box has cavity, and top is equipped with opening;Drive component is located in the inside of cavity, for driving fish;Partition component is located in box, for switching between first state and second state, in first state, partition component divides cavity into not intercommunicating feeding area and medicated bath area;In second state, feeding area and medicated bath area are communicated.The utility model solves the defect that repeated fishing operation leads to stress reaction intensification in prior art, realizes the rehabilitation nursing operation of exempting from transfer processing, improves goldfish recovery quality and efficiency.

1. The name of the design product: lampshade of lantern (goldfish). 2. The use of the design product: lampshade of lantern. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

The application discloses a method for creating a double-tail phenotype by using a cytosine single-base editing system to inactivate a goldfish szla gene and application thereof; a target sequence corresponding to sgRNA in the cytosine single-base editing system is shown as SEQ ID NO:1, a guide sequence is shown as SEQ ID NO:4, and a skeleton sequence is shown as SEQ ID NO:5. The method comprises the following steps: (1) obtaining sgRNA of the cytosine single-base editing system; (2) introducing the cytosine single-base editing system; and (3) performing phenotype analysis. The application has the advantages of precise target selection, clear editing result, predictable mutation type, convenient molecular detection and high phenotype induction efficiency, and can be used for precise creation of double-tail goldfish, development function research of a goldfish tail fin and molecular design breeding of ornamental fish.

The invention provides a method for realizing efficient genome integration of an exogenous DNA fragment and application of the method. The method comprises the following steps: construction of a transgenic vector, preparation of ZB transposon enzyme, transgenic microinjection and fluorescence signal detection. According to the invention, ZB transposon from zebra fish is utilized to construct p-miniZB plasmid, a minimum empty vector sequence is provided to meet the cloning requirements of different exogenous genes, the maximum assembly of exogenous fragments is facilitated, transposase is optimized through goldfish codon preference, nuclear localization signals are recombined at the N terminal and the C terminal of protein, and the transposase can be used for cloning different exogenous genes. The coding positioning integration of exogenous DNA fragments is facilitated, the integration rate and hereditary rate of exogenous genes can be improved, the probability of gene jumping of goldfish homologous transposase systems and transposase can be reduced, and the method has the characteristics of convenience in operation, high conversion rate, high stability, high precision and the like, can be applied to multiple biotechnical fields, and has wide application prospects. And a new thought and a new method are provided for related research and utilization of fish transgenosis.

1. Name of the designed product: comb (goldfish). 2. Use of the designed product: for combing hair. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The invention belongs to the technical field of animal genetic engineering and biological breeding, and particularly relates to a recombinant plasmid for co-expressing human antibacterial peptide and human interferon and application of the recombinant plasmid in the aspect of improving the disease resistance of aquarium fish. The recombinant plasmid is a plasmid which contains a human antibacterial peptide gene hLL-37 and a human interferon gene hIFNA1 and has a Tol2 transposon, a goldfish pan-expression promoter gActb1, a green fluorescent protein gene EGFP and a polyA sequence, and the nucleotide sequence of the recombinant plasmid is as shown in SEQ ID NO. 1. The recombinant plasmid and the Tol2 transposase mRNA are jointly introduced into the aquarium fish, so that the antiviral capability and the antibacterial capability of the aquarium fish can be improved.

The present invention relates to the technical field of goldfish breeding techniques, and particularly to a breeding method for telescope-eye goldfish. The breeding method for telescope-eye goldfish is divided into an incubation stage, a first cultivation stage, and a second cultivation stage, and includes the following steps: 1) Incubation stage; breeding and hatching telescope-eye goldfish; 2) First cultivation stage; after the fry start to feed, gradually and slowly raise the temperature of the breeding water body from room temperature until the preset temperature is reached, and maintain the preset temperature for cultivation; 3) Second cultivation stage; after cultivating for a preset time, when the proportion of the eyeballs of the telescope-eye goldfish in the whole body reaches the preset proportion, gradually and slowly lower the temperature of the breeding water body until it reaches room temperature. This breeding method for telescope-eye goldfish can effectively increase the proportion of the eyeballs of the telescope-eye goldfish in the whole body in actual breeding. The cultivated telescope-eye goldfish can meet the aesthetic needs of the market. At the same time, this provides basic experimental materials for the research on the eye development of telescope-eye goldfish.

1. Name of the product in this design: Swimming ring (goldfish). 2. Intended use of this design: for water sports or recreation. 3. The key design feature of this product is its shape. 4. The picture or photo that best illustrates the key design points: Design 1 3D diagram 1. 5. Design 1 is designated as the basic design. 6. Other situations requiring explanation: Both A and B are transparent.