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A Dexamethasone Efficient Degradation Bacteria and Its Application

A technology of dexamethasone and Rhodococcus, applied in bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., can solve problems such as limited removal capacity, and achieve the effect of fast degradation rate and promotion of degradation

Active Publication Date: 2022-03-22
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic steroid hormone substances such as dexamethasone are frequently detected in urban sewage treatment plants, farms, groundwater, surface water and other environmental water bodies, indicating that the existing sewage treatment process has limited removal capacity for such compounds, and it is necessary to develop efficient and effective Targeted and highly applicable microbial degradation removal method

Method used

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  • A Dexamethasone Efficient Degradation Bacteria and Its Application
  • A Dexamethasone Efficient Degradation Bacteria and Its Application
  • A Dexamethasone Efficient Degradation Bacteria and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening and identification of dexamethasone-degrading bacteria

[0024] In the early stage, the flushing water of a large-scale pig farm in Hunan Province was used as the source of bacteria, and 28 kinds of single bacteria were obtained through artificial enrichment culture and purposeful separation and purification. On this basis, the present invention screens out a highly efficient degrading bacterium of dexamethasone, and the specific implementation process is as follows:

[0025] Take 59 10mL glass test tubes, add 5mL sterilized MH broth respectively, 56 of them are used as the experimental group and add 28 kinds of single bacteria that have been cultured to the logarithmic phase, each single bacteria has 2 parallels, and another 3 A test tube without bacteria served as a control group. The target glucocorticoid dexamethasone was added to the experimental group and the control group respectively, so that the final concentration was 0.5 mg / L. The test ...

Embodiment 2

[0029] Embodiment 2: Degradation of dexamethasone by Rhodococcus D32 in MH broth medium

[0030]Dispense the sterilized MH broth medium into 300mL clean Erlenmeyer flasks under aseptic conditions, add 150ml of culture medium to each Erlenmeyer flask, and add the target glucocorticoid dexamethasone (Dexamethasone, DEX) to make Its final concentration is 0.5mg / L. The experiment included the bacteria experimental group and the sterilized control group: (1) the experimental group (non-sterilized group, Nonsterile), inoculated Rhodococcus D32 into the sterilized MH broth containing the target compound and mixed evenly; (2) In the control group (sterile group, Sterile), the strain was not inoculated, and sodium azide with a mass fraction of 0.02% was added to the sterilized MH broth to inhibit the bacteria. All Erlenmeyer flasks were sealed with an air-permeable parafilm, the outer wall was wrapped with tin foil to avoid light, and placed in a shaker (30°C, 180rpm) for culture; the...

Embodiment 3

[0034] Embodiment 3: Rhodococcus D32 promotes the degradation of dexamethasone in actual sewage

[0035] Take 20ml of water sample from the anoxic process section of Guanlan Sewage Treatment Plant in Guangzhou City and put it into a 50ml sterilized Erlenmeyer flask, and add the target glucocorticoid dexamethasone (DEX) to make the final concentration 20μg / L. The experiment included the experimental group with Rhodococcus D32 and the control group without Rhodococcus D32: (1) Experimental group (bacteria added group, Sewage added strainD32), Rhodococcus D32 was inoculated into the sewage medium containing the target compound dexamethasone (2) Control group (no bacteria group, Sewage alone), using the same sewage as the experimental group, without any treatment, without adding Rhodococcus D32. After all Erlenmeyer flasks were sealed with air-permeable sealing film, they were placed in a shaker (30°C, 180rpm) for culture; the experiment was set up in three parallels, and the resi...

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Abstract

The invention discloses a dexamethasone high-efficiency degrading bacterium and its application. Rhodococcus sp. D32, deposit number: GDMCC No.61560. The bacterial strain of the present invention has a fast degradation rate to dexamethasone, can be used for degrading single dexamethasone, and can also be used for degrading mixed pollutants of multiple synthetic steroid hormones, and can be used for urban sewage and aquaculture containing synthetic steroid hormones such as dexamethasone. Wastewater treatment provides a new germplasm resource.

Description

Technical field: [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a dexamethasone highly efficient degrading bacterium and application thereof. Background technique: [0002] Dexamethasone (DEX) is an important synthetic steroid glucocorticoid drug, its biological activity is 40 times that of the natural glucocorticoid cortisol, it has strong anti-inflammatory and immunosuppressive effects, and is widely used in the medical field. Since December 2019, the new crown epidemic has swept the world and become a major health threat to human beings. Through continuous exploration and experimentation, researchers have come up with the first drug with life-saving effect in patients infected with the new crown - dexamethasone. Clinical trials have shown that DEX can shorten the hospitalization time and mortality of patients with new crowns. These research results have led to a surge in the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
CPCC02F3/34C02F2101/34
Inventor 张晋娜许玫英宫晓范邓通初
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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