Method for detecting pesticide carbaryl based on nano-gold etching

Abstract

The invention discloses a method for detecting the pesticide carbaryl based on nano-gold etching, by adding the to-be-detected solution I containing carbaryl and four-(4-pyridyl) zinc porphyrin nanorods into the solution I containing biconical gold nanoparticles In the mixed solution II of hydrogen peroxide, mix uniformly and fully react to obtain the solution system III; according to the color change of the solution system III obtained before and after the introduction of carbaryl, or determine the peak position of the corresponding biconical nano-gold LSPR peak Changes to realize the detection of carbaryl. The invention has the advantages of simple operation, high sensitivity, rich color changes, etc., and can realize rapid, quantitative and visual identification of carbaryl in food systems with complex components such as fruits, vegetables and Chinese medicinal materials, and is suitable for popularization and application.

Description

technical field

[0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a method for detecting the pesticide carbaryl based on nano-gold etching. Background technique

[0002] Carbaryl, alias carbaryl, N-methylcarbamate-1-naphthyl ester, (1-naphthyl)-N-methylcarbamate, aminocarbaphthalate bis(2-ethylhexyl) ) ester; has a certain systemic effect, and is often used to control rice planthoppers, leafhoppers, thrips, bean aphids, soybean borers, cotton bollworms, fruit tree pests, forestry pests, etc. In the process of agricultural production, in order to effectively prevent pests and diseases and improve the quality of crop production, during the process of artificially spraying carbaryl, pesticides are absorbed by the roots into the interior of plant tissues and organs, and the pesticides remaining in plant tissues and organs are less affected by the external environment and are difficult to remove by cleaning , and...

Examples

Embodiment 1

[0061] A method for the visual detection of the pesticide carbaryl based on nano-gold etching, the schematic diagram of the detection principle is shown in image 3 , including the following steps:

[0062] The carbaryl concentration was 5×10 -4 -5×10 -5 mol / L of 50 μL water matrix and 80 μL of the obtained nano-zinc porphyrin solution (the concentration of zinc porphyrin rods is 9.6×10 -6 mol / L) mixed evenly, the resulting mixed solution I was added to a 96-well plate containing 150 μL of the resulting biconical gold nanometer solution (0.9575 g / ml) and 20 μL of hydrogen peroxide (3mol / L) mixed solution, and supplemented with water Until the volume of the obtained solution system is 400 μL, react at 40°C for 45 minutes, use a smart phone to take pictures of the color changes of the solution in the 96-well plate before and after the introduction of different concentrations of carbaryl, and visually observe the addition of different concentrations of carbaryl. After the colo...

Embodiment 2

[0065] A method for quantitatively detecting the pesticide carbaryl in a water matrix based on nano-gold etching, specifically comprising the following steps:

[0066] The carbaryl concentration was (5 × 10 -5 mol / L, 7×10 -5 mol / L, 1×10 -4 mol / L, 1.5×10 -4 mol / L, 2×10 -4 mol / L, 2.5×10 -4 mol / L, 5×10 -4 mol / L) of 50 μL water matrix and 100 μL of the resulting nano-zinc porphyrin solution (the concentration of zinc porphyrin rods is 9.6×10 -6 mol / L) mixed evenly, the resulting mixed solution was added to the mixed solution containing 100 μL of the resulting biconical gold nanometer solution (0.9575g / ml) and 150 μL of hydrogen peroxide (3mol / L), and added to the resulting solution system with water The volume is 600μL, react at 40°C for 45min, and then measure the UV-visible spectrum in the range of 400-1000nm, respectively measure (three times in parallel) under the same zinc porphyrin rod concentration, the corresponding nano-gold LSPR peak before and after the introducti...

Embodiment 3

[0071] A method for the quantitative detection of the pesticide carbaryl in a complex matrix based on nano-gold etching. The obtained nano-zinc porphyrin and biconical nano-gold are applied to the detection of carbaryl in fruits, vegetables, Chinese medicinal materials and soil matrices, specifically including Follow the steps below:

[0072] Add 50 μL of carbaryl to a concentration of 7×10 -5 mol / L, 1.5×10 -4 mol / L, 2.5×10 -4 mol / L of different substrates (apple, cabbage, chrysanthemum, lily, Atractylodes macrocephala, soil) and 100 μL of the resulting nano-zinc porphyrin solution (concentration of 9.6×10 - 6mol / L) mixed for 1 min, the mixed solution was added to the mixed solution containing 100 μL of the obtained biconical gold nanometer solution (0.9575 g / ml) and 150 μL of hydrogen peroxide (3mol / L), and supplemented with water to the volume of the obtained solution system was 600 μ L, reacted at 40°C for 45min, then carried out UV-visible spectrometry in the range of ...

technical field

[0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a method for detecting the pesticide carbaryl based on nano-gold etching. Background technique

[0002] Carbaryl, alias carbaryl, N-methylcarbamate-1-naphthyl ester, (1-naphthyl)-N-methylcarbamate, aminocarbaphthalate bis(2-ethylhexyl) ) ester; has a certain systemic effect, and is often used to control rice planthoppers, leafhoppers, thrips, bean aphids, soybean borers, cotton bollworms, fruit tree pests, forestry pests, etc. In the process of agricultural production, in order to effectively prevent pests and diseases and improve the quality of crop production, during the process of artificially spraying carbaryl, pesticides are absorbed by the roots into the interior of plant tissues and organs, and the pesticides remaining in plant tissues and organs are less affected by the external environment and are difficult to remove by cleaning , and...

Examples

Embodiment 1

[0061] A method for the visual detection of the pesticide carbaryl based on nano-gold etching, the schematic diagram of the detection principle is shown in image 3 , including the following steps:

[0062] The carbaryl concentration was 5×10 -4 -5×10 -5 mol / L of 50 μL water matrix and 80 μL of the obtained nano-zinc porphyrin solution (the concentration of zinc porphyrin rods is 9.6×10 -6 mol / L) mixed evenly, the resulting mixed solution I was added to a 96-well plate containing 150 μL of the resulting biconical gold nanometer solution (0.9575 g / ml) and 20 μL of hydrogen peroxide (3mol / L) mixed solution, and supplemented with water Until the volume of the obtained solution system is 400 μL, react at 40°C for 45 minutes, use a smart phone to take pictures of the color changes of the solution in the 96-well plate before and after the introduction of different concentrations of carbaryl, and visually observe the addition of different concentrations of carbaryl. After the colo...

Embodiment 2

[0065] A method for quantitatively detecting the pesticide carbaryl in a water matrix based on nano-gold etching, specifically comprising the following steps:

[0066] The carbaryl concentration was (5 × 10 -5 mol / L, 7×10 -5 mol / L, 1×10 -4 mol / L, 1.5×10 -4 mol / L, 2×10 -4 mol / L, 2.5×10 -4 mol / L, 5×10 -4 mol / L) of 50 μL water matrix and 100 μL of the resulting nano-zinc porphyrin solution (the concentration of zinc porphyrin rods is 9.6×10 -6 mol / L) mixed evenly, the resulting mixed solution was added to the mixed solution containing 100 μL of the resulting biconical gold nanometer solution (0.9575g / ml) and 150 μL of hydrogen peroxide (3mol / L), and added to the resulting solution system with water The volume is 600μL, react at 40°C for 45min, and then measure the UV-visible spectrum in the range of 400-1000nm, respectively measure (three times in parallel) under the same zinc porphyrin rod concentration, the corresponding nano-gold LSPR peak before and after the introducti...

Embodiment 3

[0071] A method for the quantitative detection of the pesticide carbaryl in a complex matrix based on nano-gold etching. The obtained nano-zinc porphyrin and biconical nano-gold are applied to the detection of carbaryl in fruits, vegetables, Chinese medicinal materials and soil matrices, specifically including Follow the steps below:

[0072] Add 50 μL of carbaryl to a concentration of 7×10 -5 mol / L, 1.5×10 -4 mol / L, 2.5×10 -4 mol / L of different substrates (apple, cabbage, chrysanthemum, lily, Atractylodes macrocephala, soil) and 100 μL of the resulting nano-zinc porphyrin solution (concentration of 9.6×10 - 6mol / L) mixed for 1 min, the mixed solution was added to the mixed solution containing 100 μL of the obtained biconical gold nanometer solution (0.9575 g / ml) and 150 μL of hydrogen peroxide (3mol / L), and supplemented with water to the volume of the obtained solution system was 600 μ L, reacted at 40°C for 45min, then carried out UV-visible spectrometry in the range of ...

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