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A low-cost, rapid micro-propagation method for excellent seedlings of Eucommia ulmoides

A low-cost and excellent technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of long reproduction cycle, reproduction is limited by season and time, achieve vigorous growth, shorten reproduction cycle, Avoid cumbersome effects

Active Publication Date: 2022-07-29
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to solve the problem that the existing eucommia breeding method has a long breeding cycle and the breeding is limited by seasons and time

Method used

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  • A low-cost, rapid micro-propagation method for excellent seedlings of Eucommia ulmoides
  • A low-cost, rapid micro-propagation method for excellent seedlings of Eucommia ulmoides
  • A low-cost, rapid micro-propagation method for excellent seedlings of Eucommia ulmoides

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A low-cost, rapid and micro-propagation method for fine seedlings of Eucommia ulmoides, comprising the following steps:

[0043] (1) Take material

[0044] From the beginning of April to the beginning of May, the Eucommia ulmoides with one leaf and one bud that grows in the same year and has a length of 2.0-3.0 cm with excellent agronomic traits (one or several traits of high yield, large leaves, fast growth, and strong stress resistance) are selected in the field. The robust microstem segments are explants;

[0045] (2) Pretreatment of explants

[0046] After the stem section in step (1) is rinsed with running water, it is immersed in low-temperature plasma activated water for 2 min, and then taken out for subsequent use;

[0047] (3) Induction treatment of adventitious roots of explants

[0048] The pretreated stem base in step (2) is placed in the adventitious root induction solution for 30s, wherein the adventitious root induction solution contains sodium nitropr...

Embodiment 2

[0055] A low-cost, rapid and micro-propagation method for fine seedlings of Eucommia ulmoides, comprising the following steps:

[0056] (1) Take material

[0057] From the first ten days of May to the first ten days of June, the perennial Eucommia ulmoides with excellent agronomic traits (one or several traits of high yield, large leaves, fast growth and strong stress resistance) were selected in the field with one leaf and one bud. 3.0cm robust microstem segments are explants;

[0058] (2) Pretreatment of explants

[0059] After the stem section in the step (1) is rinsed with running water, it is immersed in low-temperature plasma activated water for 4 min, and then taken out for subsequent use;

[0060] (3) Induction treatment of adventitious roots of explants

[0061] The stem section base after disinfection in step (2) is placed in the adventitious root inducing solution for 30s, wherein the adventitious root inducing solution is a final concentration of 400mg / L sodium ...

Embodiment 3

[0069] A low-cost, rapid and micro-propagation method for fine seedlings of Eucommia ulmoides, comprising the following steps:

[0070] (1) Take material

[0071] From the end of June to the end of July, the perennial Eucommia ulmoides with excellent agronomic traits (one or several traits of high yield, large leaves, fast growth and strong stress resistance) were selected in the field with one leaf and one bud. 3.0cm robust microstem segments are explants;

[0072] (2) Pretreatment of explants

[0073] After the stem section in the step (1) is rinsed with running water, it is immersed in low-temperature plasma activated water for 6 min, and then taken out for subsequent use;

[0074] (3) Induction treatment of adventitious roots of explants

[0075] The stem segment base after disinfection in the step (2) is placed in the adventitious root induction solution for 30s, wherein the adventitious root induction solution is a final concentration of 500 mg / L of sodium nitroprussi...

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Abstract

The invention discloses a low-cost and rapid micro-propagation method for excellent seedlings of Eucommia ulmoides. The invention uses the micro-stem segment with one leaf and one bud of the Eucommia strain as the explant, and undergoes pretreatment, adventitious root induction treatment, colonization and Steps such as adventitious root induction culture, axillary bud germination induction of explants and seedling culture. The rooting inducer used in the present invention treats the stem segment for 30s, 7-10d is accompanied by the formation of adventitious root primordium, and a complete regenerated plant of Eucommia can be obtained in 22d, which is faster than using traditional propagation methods and tissue culture technology to propagate Eucommia. Fast, the cycle of reproduction is greatly shortened; and the present invention directly takes root and forms seedlings in the greenhouse by cutting the micro-stem segment in the seedling tray equipped with the nutrient matrix, on the one hand, avoids the influence and restriction of natural factors such as seasons and regions, On the other hand, it also avoids the cumbersome process of tissue culture and propagation of Eucommia ulmoides and the defects of low survival rate of subsequent tissue culture regeneration seedlings domestication and transplantation.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, and more particularly, to a low-cost and rapid micro-propagation method for excellent seedlings of Eucommia ulmoides. Background technique [0002] Eucommia ulmoides Oliver, also known as Sizhong and Silky Wood, belongs to the Eucommia family, Eucommia is a perennial woody plant, which is a unique economic plant and medicinal plant in my country. Living fossil plants. Eucommia is extremely adaptable, resistant to cold, drought, salinity, and pests. It is an ideal tree species for urban and rural greening. It plays an important role in watershed management, soil and water conservation, greening of barren hills, and greening of passages and urban streets. [0003] Eucommia ulmoides, as a unique medicinal material in my country, is used in "three lowerings" (lowering blood pressure, lowering blood sugar, lowering blood lipids), "four protections" (protecting cardiovascular, live...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 侯金艳吴丽芳王大成苏鹏飞王萍丁双双
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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