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Application of metabonomics analysis technology to discriminating spatiotemporal classification of Danxiangxiangduncong tea

A technology of metabolomics and single-clump tea, which is applied in the application field of metabolomics analysis technology to distinguish the time-space classification of duck feces-flavored single-clump tea, and can solve problems such as inability to apply and provide technical guidance

Active Publication Date: 2021-07-16
广东省农业科学院农业质量标准与监测技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the identification of characteristic components in different grades of Yashixiang Dancong tea with metabolomics analysis methods and the application of time and space classification to the authenticity identification, and it is impossible to provide technical guidance for related applications

Method used

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  • Application of metabonomics analysis technology to discriminating spatiotemporal classification of Danxiangxiangduncong tea
  • Application of metabonomics analysis technology to discriminating spatiotemporal classification of Danxiangxiangduncong tea
  • Application of metabonomics analysis technology to discriminating spatiotemporal classification of Danxiangxiangduncong tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A metabolomic analysis method for identifying the characteristic components of Yashixiang Dancong tea:

[0049] 1) Sample preparation

[0050] The selected tea varieties are Yashixiang, and the altitude information of each Yashixiang is recorded. All samples are collected with 3 replicates. Among them, the altitude of Yashixiang is divided into three categories, which are recorded as low camellia (below 500 meters), medium camellia (500-700 meters) and high camellia (above 700 meters). According to the proportion of planting area at different altitudes, a total of 76 samples were collected from low mountains, 58 samples from middle mountains, and 25 samples from high mountains, totaling 159 samples.

[0051] The selected tea leaves were evenly sampled about 100g and crushed with a grinder, passed through a 60-mesh sieve and stored in a -20°C refrigerator for testing. Weigh 100mg of tea powder sample into a 1.5mL centrifuge tube, add 1mL of methanol / water (75 / 25, v / v) ...

Embodiment 2

[0070] A metabolomics analysis method for distinguishing the characteristic components of Yashixiang Dancong tea, which can be applied to distinguish the characteristic components of Yashixiang Dancong tea in different seasons. The specific steps are the same as in Example 1, except that the samples The altitude information is different. In this example, the tea varieties are Yashixiang. Among them, the altitude of Yashixiang is divided into 4 categories, which are respectively recorded as spring tea, summer tea, autumn tea and snow flakes (tea picked in winter).

[0071] The results showed that there were significant differences in the aroma of duck droppings in different seasons. ∣Log 2 Fold Change∣>1, and adj.p1 is the threshold, and 17 iso-metabolites such as D-proline, eucalyptol, and astragalin were screened out, as shown in Table 3.

[0072] Table 3 Information of 17 compounds identified based on UHPLC Q-Exactive Orbitrap MS technology

[0073]

[0074]

Embodiment 3

[0076] A metabolomics analysis method for distinguishing the characteristic components of Yashixiang Dancong tea, which can be applied to distinguish the characteristic components of Yashixiang Dancong tea from different altitudes and seasons. The specific steps are the same as those in Example 1. Because the sample also records the altitude and picking season information of Yashixiang. In this example, it can be used to identify different grades of Yashixiang such as high mountain spring tea, low mountain spring tea or low mountain snow flakes.

[0077] ∣Log 2Fold Change∣>1, and adj.p1 is the threshold, and the 8 differential metabolites screened out can be used to characterize the altitude and season information of Yashixiang.

[0078] Table 4 Information of 8 compounds identified based on UHPLC Q-Exactive Orbitrap MS technology

[0079]

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Abstract

The invention belongs to the technical field of oolong tea discrimination and particularly relates to an application of a metabonomics analysis technology to discrimination of space-time classification of Danxiang Dancong tea. In the metabonomics analysis technology, the characteristic components of the Danxiang Dancong tea at different altitudes and seasons are judged through Log2 Fold Change, adj.p and VIP values; the screening conditions for distinguishing the characteristic components of the duck manure fragrant Dancong tea are |Log2 Fold Channel| > 1.5, adj.p < 0.05 and VIP> 1; spatial and temporal classification and discrimination of Danxiang Dancong tea are carried out based on peak areas of characteristic markers, the sample amount ratio of a training set to a test set is 2: 1, a model prediction value gt greater than 0.35 is discriminated and analyzed, and the model prediction value is the maximum value in different classification values, so predicted duck shit fragrance classification is regarded as the corresponding space-time classification. According to the method, a space-time classification and discrimination model of the Danxiangxiang Dancong tea is established on the basis of different space-time Danxiangxiang marker contents.

Description

technical field [0001] The invention belongs to the technical field of oolong tea discrimination, and in particular relates to the application of a metabolomics analysis technology to discriminate the time-space classification of duck feces-flavored single cluster tea. Background technique [0002] Yashixiang Dancong tea (hereinafter referred to as "Yuckshixiang") is a high-value variety with special charm in the geographical indication product Fenghuang Dancong tea. Well, much loved by consumers. Although it is the most widely planted variety in the local area, the supply is still in short supply. In addition, the prices of different varieties and grades of tea are different. Driven by economic benefits, there are always some unscrupulous traders in the market who shoddy and faked the real ones. [0003] The traditional identification method is to evaluate and judge the characteristics of tea through sensory evaluation, which is highly subjective. The chemical composition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/72G01N30/8634G01N30/8693Y04S30/12Y02T90/167
Inventor 赵洁刘雯雯张欣江棋陈岩苏园王旭王富华
Owner 广东省农业科学院农业质量标准与监测技术研究所
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