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Application of a Metabonomics Analysis Technology to Identify Yashixiang Dancong Tea

A technology of metabolomics and Dancong tea, which is applied in the field of oolong tea identification, can solve the problems that cannot be applied and provide technical guidance

Active Publication Date: 2022-01-04
广东省农业科学院农业质量标准与监测技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the non-targeted metabolic analysis and detection method of Yashixiang Dancong tea, and it is impossible to provide technical guidance for related applications

Method used

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  • Application of a Metabonomics Analysis Technology to Identify Yashixiang Dancong Tea
  • Application of a Metabonomics Analysis Technology to Identify Yashixiang Dancong Tea
  • Application of a Metabonomics Analysis Technology to Identify Yashixiang Dancong Tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A metabolomic analysis method for identifying the characteristic components of Yashixiang Dancong tea:

[0051] 1) Sample preparation

[0052] The selected tea varieties are Yashixiang, Dawuye, Huangzhixiang, Milanxiang, Zhilanxiang, Lingtou Dancong tea and Fujian Tieguanyin. Among them, except for Tieguanyin, the other 6 kinds are all Dancong tea varieties; 6 finished tea blends were collected for each variety, and all samples were collected with 3 replicates.

[0053] The selected tea leaves were evenly sampled about 100g and crushed with a grinder, passed through a 60-mesh sieve and stored in a -20°C refrigerator for testing. Weigh 100mg of tea powder sample into a 1.5mL centrifuge tube, add 1mL of methanol / water (75 / 25, v / v) solution containing 1% formic acid; ultrasonicate the mixture for 10min at 20°C, and then use a low-temperature centrifuge Centrifuge at 11,000 rpm for 10 min at 4°C; take the supernatant, pass it through a 0.22 μm organic filter membrane, and...

Embodiment 2

[0074] A metabolomic analysis method for identifying the characteristic components of Yashixiang Dancong tea. Its specific steps are the same as in Example 1, the difference lies in the difference in sample size and extractant. Specifically, 1g of tea powder is placed in a 50mL centrifuge tube , add 25mL of 100℃ hot water.

[0075] Such as Figure 6 As shown, the upper figure and the lower figure in the figure are positive and negative ion modes respectively. Compared with the pretreatment method of embodiment 1, the characteristic peak separation effect of the total ion figure obtained by the sample of embodiment 2 is relatively poor, and the partial characteristic peak The response is lower than that of Example 1, especially in negative ion mode.

Embodiment 3

[0077] A metabolomic analysis method for identifying the characteristic components of Yashixiang Dancong tea, which is the same as the specific steps in Example 1, the difference is that the source data for screening differences is to select all the extracted peaks or use mzVault or mzCloud to match the two Metabolites with a mass spectrum score greater than 70.

[0078] The metabolites with a score greater than 70 were used for data analysis: the 6 differential metabolites screened out were the characteristic markers of Yashixiang. The peak areas of the above compounds. Compare the result with the peak area of ​​the compound in the duck feces sample, and check |Log 2 Whether the Fold Change | values ​​are greater than 1.5 can be used to judge whether it is duck droppings.

[0079] As described in Table 2, Example 3 will reduce the number of characteristic markers, and all 6 compounds such as L-arginine, L-tyrosine, and kaempferol-3-rhamnoside are obtained by screening with...

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Abstract

The invention belongs to the technical field of oolong tea identification, and relates to the application of a metabolomics analysis technique to identify Yashixiang Dancong tea. In the metabolomics analysis technique, the Log 2 Fold Change, adj .p , VIP value to identify the characteristic components of Yashixiang Dancong tea, the screening condition is ∣Log 2 Fold Change∣>1.5, adj .p <0.01 and VIP>1. A comprehensive, non-discriminatory, non-targeted metabolomics analysis method for Yashixiang was established using metabolomics analysis technology, which can analyze the metabolic components of different varieties of tea and accurately find out the characteristic components of Yashixiang; Based on the chemical composition, a metabolomics analysis method for the characteristic components of Yashixiang Dancong tea was established. Combined with chemometric analysis, screening from big data is conducive to the identification of true and false, geographical traceability and high-quality variety screening of Yashixiang. Characteristic markers of duck feces aroma for applications such as flavor regulation and so on.

Description

technical field [0001] The invention belongs to the technical field of oolong tea identification, and in particular relates to the application of a metabolomics analysis technology to identify Yashixiang Dancong tea. Background technique [0002] Yashixiang Dancong tea (hereinafter referred to as "Yuckshixiang") is a high-value variety with special charm in the geographical indication product Fenghuang Dancong tea. Well, much loved by consumers. Although it is the most widely planted variety in the local area, the supply is still in short supply. In addition, the prices of different varieties and grades of tea are different. Driven by economic benefits, there are always some unscrupulous traders in the market who shoddy and faked the real ones. [0003] The traditional identification method is to evaluate and identify the characteristics of tea through sensory evaluation, which is highly subjective. The chemical composition of tea determines the drinking value and characte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634
Inventor 赵洁刘香香陈岩刘雯雯张欣王旭王富华
Owner 广东省农业科学院农业质量标准与监测技术研究所
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