DNA methylation marker and application thereof

A methylation marker and methylation technology, applied in the field of bioengineering, can solve the problems of no reported methylation markers to prevent and control hypertension and stroke intervention targets

Active Publication Date: 2021-09-10
SUZHOU UNIV
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Problems solved by technology

[0004] At present, there is no research report on the relationship between DNA methylation of ANP coding gene NPPA and hypertension and stroke, and there is no report on a suitable methylation marker that can be used as an intervention target for the prevention and control of hypertension and stroke. point

Method used

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  • DNA methylation marker and application thereof
  • DNA methylation marker and application thereof
  • DNA methylation marker and application thereof

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Embodiment

[0034] In two independent populations, a discovery study (enrolling 2,498 individuals, including 1,109 hypertensives and 1,389 normotensives) and a validation study (enrolling 1,771 individuals, including 995 hypertensives and normotensives 776 people). Whole blood DNA samples were extracted from each subject, and the target region sequencing technology was used to detect the methylation level of each CpG site in the promoter region of the NPPA gene, that is, the promoter region of the human NPPA gene (gene number: ENSG00000175206) was queried using the ENSEMBL database , this region is Chromosome 1:11908117-11908380 (GRCh37.P13, distance from TSS: -540bp to-277bp), intercept the nucleic acid sequence of this region on NCBI, import the nucleic acid sequence into EMBOSS Cpgplot software to predict the CpG island, and then use the Epidesigner program Primers were designed for the sequences of CpG islands and CpG-intensive regions, and appropriate primers were selected for DNA me...

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Abstract

The invention relates to a DNA methylation marker. The DNA methylation marker is a methylation site Chr1: 11908353 of an NPPA gene promoter region, and the methylation degree of the DNA methylation marker indicates the onset risk of hypertension or cerebral apoplexy. The DNA methylation marker provided by the invention can be used for predicting the onset risk of cerebral apoplexy, provides a basis for screening high-risk groups of cerebral apoplexy, and can also be used as an intervention target for preventing and controlling hypertension and cerebral apoplexy. The DNA methylation marker not only contributes to explaining the molecular mechanism of the atrial natriuretic peptide acting on hypertension and cerebral apoplexy, but also provides important epidemiological evidence for NPPA gene methylation as a drug target for preventing and controlling hypertension and cerebral apoplexy.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a DNA methylation marker and its application. Background technique [0002] Hypertension is not only a cardiovascular disease, but also an important risk factor for many other cardiovascular and cerebrovascular diseases. Globally, 67% of heart disease, 54% of stroke are caused by high blood pressure, and 13.5% of deaths are attributed to high blood pressure. Among them, stroke, as a global health problem, has become the primary disease that threatens the life and health of Chinese residents, and brings huge disease burden and economic loss to Chinese residents every year. The results of the "China Stroke Epidemic Report" released by the China Stroke Association for the first time in 2015 show that there are about 2.7 million new patients with cerebrovascular diseases in my country every year, about 1.3 million patients die of cerebrovascular diseases every year, and one p...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154Y02A90/10
Inventor 彭浩张明芝何艳
Owner SUZHOU UNIV
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