Preparation method and agrobacterium tumefaciens transformation method of sterile pineapple explant

Abstract

The invention provides a preparation method and an agrobacterium transformation method of a sterile pineapple explant. The preparation method of the sterile pineapple explant comprises the following steps: firstly washing a tender pineapple plant with clear water, then respectively disinfecting with 75% alcohol and 0.1% HgCl2, inducing generation of pineapple adventitious buds through a pineapple adventitious bud induction culture medium, transferring the generated adventitious buds to an adventitious bud propagation culture medium, performing continuous illumination culture to obtain sterile pineapple plants, transferring the sterile pineapple plants to a strong seedling culture medium, performing continuous illumination culture to obtain relatively thick and strong sterile pineapple plants, and cutting the sterile pineapple plants into stem discs with the thickness of about 3 mm for pineapple agrobacterium transformation. According to the method, the sterile plants are obtained by optimizing an explant disinfection method, a large number of pineapple stem wafers are obtained through an adventitious bud way, pineapple transformation is carried out on the basis of the stem wafers, and the pineapple transformation efficiency is improved; and the method is simple in required equipment and easy to master in operation technology, and has wide development and application prospects.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and in particular relates to a method for preparing sterile pineapple explants and an Agrobacterium transformation method thereof. Background technique [0002] Pineapple (Ananas comosus) is the third most important fruit crop in the tropics and subtropics, after bananas and citrus. Pineapples are native to the Amazon River Basin in Brazil and Paraguay in South America, and were introduced to China from Brazil in the 16th century. It has now spread throughout the tropics. As a fresh food, pineapple is sweet and sour, crisp and juicy. In addition to eating fresh, pineapple is mostly used for canning, and it is widely loved because it can maintain its original flavor. Processed products Canned pineapple is known as "international canned fruit", and it can also be made into a variety of processed products, which are widely welcomed by the public. The fibers of the leaves are tough and ar...

AI Technical Summary

Problems solved by technology

[0003] Although hybridization of self-incompatible monocot species has been widely used to produce improved plant varieties, high levels of genomic heterozygosity and genomic instability make it difficult to improve plant varieties by this method

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