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Serum metabonomics analysis method for rats treating hypertension caused by high fat diet

A rat serum and metabolomics technology, applied in the field of analytical chemistry, can solve the problems of unclear specific role and complex mechanism of action, and achieve the effect of avoiding non-independence

Pending Publication Date: 2021-11-16
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the diverse ingredients and complex mechanism of action of Banxia Baizhu Tianma Decoction, its specific role in the treatment of hypertension is still unclear. Therefore, it is necessary to further clarify the role of each drug and optimize the prescription

Method used

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  • Serum metabonomics analysis method for rats treating hypertension caused by high fat diet
  • Serum metabonomics analysis method for rats treating hypertension caused by high fat diet
  • Serum metabonomics analysis method for rats treating hypertension caused by high fat diet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Experimental animal and modeling process

[0038] Sixty male SPF grade SD rats (body weight 200±20g) were given standard feed and deionized water in a normal environment (temperature 18-26°C, humidity 40%-70%, dark cycle 12 / 12 hours), and raised 1 week. After 1 week, 50 rats were randomly selected to establish a hypertensive animal model. The rats were fed with high-fat and high-sugar feed and normal saline in a humid environment (temperature 18-20°C, humidity 85%-90%) for 4 weeks. , fasting for 12 hours, body weight and blood pressure were measured and recorded. When the systolic blood pressure ≥ 140mmHg, diastolic blood pressure ≥ 90mmHg, accompanied by symptoms such as obesity, lethargy, laziness, and irregular stool, the model was successfully established.

Embodiment 2

[0039] Example 2: Preparation of the whole prescription and missing prescription of Banxia Baizhu Tianma Decoction

[0040] The whole prescription group: 20 parts of pinellia, 30 parts of Atractylodes macrocephala, 15 parts of Gastrodia elata, 15 parts of orange red, 15 parts of poria cocos, 10 parts of licorice, boiled for half an hour with the method of "boiling first, then slow frying with slow fire", decocting twice, Filter, combine the medicinal decoction, and concentrate the medicinal decoction to 2.83g / mL;

[0041] Drug deficiency group: 30 parts of Atractylodes macrocephala, 15 parts of orange red, 15 parts of Poria cocos, 10 parts of licorice, decocted for half an hour with the method of "boiling first and then decocting slowly", decocting twice, filtering, combining the medicinal soup, and concentrating the medicine Soup to 1.91g / mL;

[0042] Quechen medicine group: 20 parts of pinellia, 15 parts of Gastrodia elata, 15 parts of orange red, 15 parts of poria cocos, 1...

Embodiment 3

[0044] Embodiment 3: drug treatment

[0045] The model rats were randomly divided into 5 groups, model group (group B), whole prescription group (group C), monarch drug group (group D), minister drug group (group E), adjuvant drug group ( F group); another 10 normal rats served as the control group (A group).

[0046] Rats in groups C, D, E and F were orally administered 1 mL each of the whole formula, Quejun formula, Quechen formula, and Quezuoshi formula every day for 4 weeks. Rats in the 6 groups lived in a normal environment and were fed with standard feed and deionized water for 4 weeks.

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Abstract

The invention discloses a serum metabonomics analysis method for treating rats with hypertension caused by high fat diet, which comprises the following steps: establishing a hypertensive rat model, preparing a rhizoma pinelliae, rhizoma atractylodis macrocephalae and gastrodia elata decoction whole prescription and deficiency prescription, treating, collecting and preparing normal rat serum, hypertensive rat serum and drug-intervened hypertensive rat serum samples, treating, detecting through GC / MS (Gas Chromatography / Mass Spectrometry), analyzing the data to obtain a metabolome profile, establishing a dual cross validation screening model, screening differential metabolic markers for treating rat hypertension caused by high fat diet in the whole prescription and deficiency prescription of the rhizoma pinelliae, rhizoma atractylodis macrocephalae and gastrodia elata decoction, and analyzing potential metabolic pathways on line through a MetaboAnalyst system.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a serum metabolomics analysis method for treating hypertensive rats induced by a high-fat diet. Background technique [0002] Hypertension is a major public health problem throughout the world. An estimated 1.39 billion people worldwide suffered from hypertension in 2010. The prevalence of hypertension in my country is high, and the treatment and management rate is low. Over the past few decades, the incidence of hypertension in my country has increased significantly, especially in rural areas. Obesity, age, and lifestyle habits are common risk factors for high blood pressure. Hypertension is also a major and independent risk factor for cardiovascular disease, and clinical trials have shown that lowering blood pressure can reduce cardiovascular disease and premature death. Many pharmaceutical agents have been approved for the treatment of hypertension...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N33/50
CPCG01N30/02G01N30/72G01N33/5091
Inventor 王淑玲章洪华徐男李欣岳田庆常门连汇杨彗敏李敏慧王悦罗汝婳
Owner HANGZHOU NORMAL UNIVERSITY
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