A method for plasma metabolomic analysis of patients with primary osteoporosis based on sleep disorders

Abstract

The invention discloses a method for analyzing plasma metabolomics of patients with primary osteoporosis based on sleep disorders. The samples are prepared and collected from the plasma samples of primary osteoporosis patients with sleep disorders treated by sleep cognitive behavior. Then prepare for ultra-high performance liquid chromatography-mass spectrometry detection and analysis; sleep disorders are determined according to the Pittsburgh sleep quality index scale; ultra-high performance liquid chromatography-mass spectrometry detection and analysis: serum samples are separated by chromatographic columns and analyzed by mass spectrometry; data analysis Extract and align the original data of the instrument to obtain data that is free from noise interference and can be used for statistical analysis. Analyze the data through orthogonal partial least squares discriminant analysis and paired sample T test to obtain the differential metabolites between the two groups, and Preliminary identification and verification of these substances combined with existing literature reports. The present invention provides favorable technical support for the early prediction and prognosis of patients with primary osteoporosis in sleep disordered groups.

Description

technical field [0001] The invention relates to the technical field of medical research, in particular to a method for analyzing plasma metabolomics of patients with primary osteoporosis based on sleep disorders. Background technique [0002] Sleep disturbance is one of the most common problems in Chinese society at present. It is mainly manifested as difficulty in falling asleep, increased number of awakenings at night, early morning awakening and inability to fall back to sleep after waking up. Long-term low-quality sleep is associated with the occurrence and development of many diseases, and is an important pathogenic factor for the occurrence of osteoporosis, which induces its occurrence and development, and osteoporosis is very prone to fractures. Fractures not only reduce the quality of life of the population, but also increase bedridden-related mortality. Reducing or lowering sleep disorders can greatly improve osteoporosis caused by low-quality sleep, which not only...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for analyzing plasma metabolomics of patients with primary osteoporosis based on sleep disorders, which adopts orthogonal partial least squares discriminant analysis (OPLS-DA) and paired sample T test for data analysis. The cross-comparison of the analysis results solves the problem that all samples will be used to build the model in the modeling process due to the advantages of independence, so there is a certain degree of non-independence in the process, which interferes with the judgment of the model's prediction ability and the model's prediction error estimate

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