Primers for detection of methylation in the promoter region of hif-1α gene and its detection method and application

A technology for detecting primers and methylation, applied in the field of genetic engineering, to achieve high sensitivity, strong subjectivity, and high accuracy

Active Publication Date: 2020-11-06
SECOND AFFILIATED HOSPITAL OF XINJIANG MEDICAL UNIV
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Problems solved by technology

[0005] At present, there is still a lack of rapid, safe, and easy-to-use methods for evaluating and predicting depression in patients with coronary heart disease.

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  • Primers for detection of methylation in the promoter region of hif-1α gene and its detection method and application
  • Primers for detection of methylation in the promoter region of hif-1α gene and its detection method and application
  • Primers for detection of methylation in the promoter region of hif-1α gene and its detection method and application

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0025] 1. Sample preparation and collection include screening of patients with coronary heart disease and depression who meet the entry requirements. Inclusion criteria (meeting all criteria): 1. Patients diagnosed with coronary atherosclerotic heart disease according to the diagnostic criteria of WHO coronary heart disease and undergoing PCI ( Have typical symptoms of angina pectoris and exclude aortic valve disease, or a clear history of old myocardial infarction,...

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Abstract

The invention provides a methylation detection primer and detection method of an HIF-1 alpha gene promoter region and an application. The methylation detection primer is characterized by comprising apair of methylation specificity amplification primers for the HIF-1 alpha gene promoter region and has the advantage that the methylation detection primer can forecast coronary heart disease complication depressed mood from the molecular level conveniently, with strong points, and besides, medicines using methylation of the HIF-1 alpha (hypoxia inducible factor-1 alpha ) gene promoter region as atarget point are hopeful to become a new means for intervention of the coronary heart disease complication depressed mood.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a primer for detecting methylation in the promoter region of the HIF-1α gene, a detection method and an application. Background technique [0002] Coronary heart disease in my country is increasing at an average rate of 5% every year, and its morbidity and mortality are also increasing year by year. It has become the leading cause of death. In the occurrence of coronary heart disease, in addition to common biological factors, the impact of depression on the incidence and prognosis of coronary heart disease is becoming more and more significant. By 2020, depression will become the second cause of death and disability in the world, second only to in coronary heart disease. At present, there is still a lack of clinical methods for evaluating and predicting depression in patients with coronary heart disease. Therefore, establishing a technical method for predicting patients' depr...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154
Inventor 王帆李慧孔田甜宋春史建平许衡刘彦隆王玉
Owner SECOND AFFILIATED HOSPITAL OF XINJIANG MEDICAL UNIV
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