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Application of joint synovial fluid biomarker ADAMDEC1

A bio-marker, synovial fluid technology, applied in biological material analysis, instruments, measurement devices, etc., can solve problems such as unknown physiological functions

Pending Publication Date: 2021-11-30
东阳市人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its physiological function is largely unknown
There is no research report on the relationship between ADAMDEC1 and arthritis

Method used

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  • Application of joint synovial fluid biomarker ADAMDEC1
  • Application of joint synovial fluid biomarker ADAMDEC1
  • Application of joint synovial fluid biomarker ADAMDEC1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Bioinformatics data screening for diagnostic biomarkers that differentiate OA and RA

[0018] Download 7 gene chip data (GSE55235, GSE55457, GSE55584, GSE12021, GSE1919, GSE7669 and GSE36700) and one RNA sequencing data (GSE89408) from NCBI GEO, and these 8 data files contain synovial tissues of OA and RA patients Gene expression data. The bioinformatics data screening of the above 8 data sets was performed using the "Bioconductor" data operating environment of the R language. Firstly, the quality inspection and analysis of each data set is carried out. If the data files in each data set do not show RNA degradation, the quality inspection is qualified. Then standardize each data set, use LIMMA, edgeR, and DESeq2 data analysis packages to find out the genes with differential expression in the synovial tissues of OA and RA patients from the above 8 data sets, and perform ROC analysis on the differentially expressed genes to screen out potential distinguishing OA and RA ...

Embodiment 2

[0020] Diagnostic performance of ADAMDEC1 in differentiating RA from OA patients

[0021] 1. Experimental samples and reagents

[0022] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0023] ADAMDEC1 protein assay reagent;

[0024] ADAMDEC1 ELISA kit was purchased from CUSABIO company.

[0025] 2. Experimental method

[0026] 1. Collection of human joint synovial fluid samples

[0027] Collect synovial fluid samples from 30 patients with OA and 14 patients with RA combined with OA who underwent joint replacement in Dongyang People's Hospital, labeled and stored in -80°C refrigerator for later use.

[0028] 2. Determination of the content of the target protein in the joint synovial fluid by ELISA

[0029] The content of ADAMDEC1 in the synovial fluid of the joints was determined strictly according to the procedure of the ELISA kit.

[0030] 3. Data processing method

[0031] The ROC curve of the target p...

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Abstract

The invention discloses a joint synovial fluid biomarker ADAMDEC1 and an application of a detection product of the joint synovial fluid biomarker ADAMDEC1 in diagnosis and differentiation of two diseases including rheumatoid arthritis and osteoarthritis. The biomarker ADAMDEC1 has a very high diagnostic value in the aspect of diagnosing and distinguishing rheumatoid arthritis and osteoarthritis, is high in diagnostic accuracy, more directly aims at local focuses compared with an existing diagnostic standard, and is easy to operate.

Description

technical field [0001] The invention belongs to the field of detection and diagnosis of disease differentiation, and relates to the application of biomarkers in the direction of detection and diagnosis of disease differentiation, in particular to the application of a joint synovial fluid biomarker ADAMDEC1 in the diagnosis and differentiation of rheumatoid arthritis and osteoarthritis application. Background technique [0002] Rheumatoid arthritis (RA) is a chronic, systemic, inflammatory disease of unknown etiology, mainly involving synovial joints, and the main symptoms are pain, stiffness (especially morning stiffness) and swelling of multiple joints. This arthritis is usually symmetrical, and if the disease is not controlled, erosion of cartilage and bone leads to destruction of the joint, causing joint deformity. In 10 to 20 years, the disease can cause severe motor disability, significant decline in quality of life, and even loss of working ability. Females are 2 to ...

Claims

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Application Information

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IPC IPC(8): G01N33/573
CPCG01N33/573G01N2800/102G01N2800/105
Inventor 康乐李勇王利宏
Owner 东阳市人民医院
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