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An up-conversion nano-diagnosis and treatment integrated platform probe and preparation method thereof

A nanocrystal and platform technology, applied in chemical instruments and methods, preparations for in vivo experiments, antibodies, etc., can solve the problems of upconversion nanoparticles Her2, etc., achieve good upconversion luminescence performance, and the preparation method is simple and easy Line, strong effect of stability

Active Publication Date: 2022-05-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no related research on the binding of Her2 to upconverting nanoparticles

Method used

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  • An up-conversion nano-diagnosis and treatment integrated platform probe and preparation method thereof
  • An up-conversion nano-diagnosis and treatment integrated platform probe and preparation method thereof
  • An up-conversion nano-diagnosis and treatment integrated platform probe and preparation method thereof

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Experimental program
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preparation example Construction

[0059] The present invention also provides a preparation method of a probe for an integrated platform for upconversion nano-diagnosis and treatment, comprising the following steps:

[0060] The NPs were coupled with HER2-targeted drugs to obtain the integrated platform probes for upconversion nano-diagnosis and therapy.

[0061] In the present invention, the coupling is preferably mixing NPs with HER2-targeted drugs pretreated by TCEP, and the coupling temperature is preferably 22-28°C, more preferably 24-26°C; the coupling The time is preferably 20 ~ 40min, more preferably 25 ~ 35min, also preferably 28 ~ 32min; preferably accompanied by vibration in the coupling process of the present invention, the rotating speed of the vibration is preferably 600 ~ 1000rpm, more preferably 700 ~900rpm, also preferably 800~850rpm. The present invention also preferably carries out ultrafiltration after described coupling finishes, and the molecular weight cut-off of described ultrafiltratio...

Embodiment 1

[0089] First add 0.5mmol LnCl 3 (Gd, Yb, Tm=80:18:2) was dissolved in 1mL of water, 2mmol of sodium fluoride was dissolved in 4mL of water, 1.2g of sodium hydroxide was weighed into a 50mL single-necked flask, and 4mL of water was added to dissolve completely, and then 9mL of Water ethanol and 20mL oleic acid, after stirring for about 10min, add LnCl drop by drop 3 aqueous solution and sodium fluoride aqueous solution, stirred for about 1 hour, added 2 times the volume of ethanol to precipitate the product, centrifuged at 10,000 rpm for 10 minutes, washed once with ethanol and cyclohexane, and dispersed the final product in 2 mL of cyclohexane to obtain NaLnF 4 Cyclohexane solutions of nanocluster precursors.

[0090] 0.5mmol LuCl 3 Dissolve in 1mL of water, 2mmol of sodium fluoride in 4mL of water, weigh 1.2g of sodium hydroxide into a 50mL one-necked flask, add 4mL of water to dissolve completely, then add 9mL of absolute ethanol and 20mL of oleic acid, stir for about 10mi...

experiment example 1

[0094] Experimental Example 1 Electron Microscope and Characterization

[0095] Take the NP-mAb prepared in Example 1, dilute it with pure water to 0.2 mg / ml, add it dropwise to the copper grid for electron microscopy, and take electron microscope pictures after sample preparation (FEI TECNAI G20).

[0096] Electron microscope picture as figure 1 As shown, the particle size statistics graph is shown in figure 2 As shown, the hydrated particle size diagram is shown in image 3 As shown, the fluorescence spectrum is shown in Figure 4 shown.

[0097] The results showed that the prepared core-shell structure UCNP was spherical, with a particle size of about 22.05±1.82nm; there was an obvious emission peak at 804nm under 980nm laser excitation; at the same time, it had Her2 activity and active targeting.

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Abstract

The invention provides an up-conversion nano-diagnosis and treatment integrated platform probe, which belongs to the field of new biomedical technology. The up-conversion nano-diagnosis and treatment integrated platform probe includes a structure in which NPs are coupled with HER2 targeting drugs. The present invention overcomes the problem of the lack of combination of upconversion nanomaterials and Her2 targeting drugs in the prior art, can fill the gaps in related research, combines the excellent performance of upconversion nanomaterials and the special properties for Her2 targets, and has Her2 activity, It has strong stability, high biological safety, simple preparation method, good up-conversion luminescence performance, and active targeting function, and is expected to be further used in the preclinical development and application of integrated probes for diagnosis and treatment. Breast cancer with high expression provides a new diagnosis and treatment strategy.

Description

technical field [0001] The invention relates to the field of new biomedical technology, in particular to an up-conversion nano-diagnosis and treatment integrated platform probe and a preparation method thereof. Background technique [0002] Breast cancer (breast cancer, BC) is a malignant tumor that occurs in the epithelial tissue of the terminal ductal lobular unit of the breast. It is a complex heterogeneous disease that can be divided into estrogen receptor positive (ER+) and Progesterone receptor positive (PR+), human epidermal growth factor receptor 2 positive (HER2+) and triple negative breast cancer (TNBC). Despite substantial advances in the prevention, diagnosis, and treatment of breast cancer in recent years, and consequent improvements in overall survival, advanced breast cancer remains an incurable disease, with 6% of new breast cancer cases ~7% of patients are initially diagnosed with advanced breast cancer, and 30% of patients initially diagnosed with early br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K47/69A61K39/395C09K11/85C09K11/02A61P35/00
CPCC09K11/7773C09K11/02C09K11/025A61K47/6923A61K39/395A61K49/0019A61K49/0058A61K49/0067A61P35/00
Inventor 朱然张川施秀敏王峰王广林梁茂林任峰李藏
Owner SUZHOU UNIV
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