Preparation of organic microporous filter screen filter paper strip and application of organic microporous filter screen filter paper strip in rapid detection of citrus yellow shoot

Abstract

The invention discloses an organic microporous filter screen filter paper strip. The organic microporous filter screen filter paper strip comprises an organic microporous filter screen; one end of the organic microporous filter screen is a handheld end, and the other end of the organic microporous filter screen is an exposed end; the handheld end is of a sandwich structure and comprises a middle organic microporous filter screen and filter paper attached to the two faces of the organic microporous filter screen; and the exposed end is only the organic microporous filter screen. A sample DNA is adsorbed in a plant tissue solution obtained by grinding and cracking in a specific buffer solution by using the organic microporous filter screen filter paper strip, impurities are removed by using a washing solution, and the adsorbed sample DNA is eluted into deionized water to complete sample DNA capture. Therefore, when the organic microporous filter screen filter paper strip is applied to an LAMP method of the citrus yellow shoot, the candidatus liberibacter asiaticus can be rapidly, simply, conveniently and economically detected in the field, and the organic microporous filter screen filter paper strip has important significance on prevention and control of the citrus yellow shoot.

Description

technical field [0001] The invention belongs to the technical field of detection of citrus huanglongbing, and in particular relates to the preparation of an organic microporous filter paper strip and its application in the rapid detection of citrus huanglongbing. Specifically, the organic microporous filter paper strip is used to capture sample DNA method and the LAMP method for detecting citrus Huanglongbing. Background technique [0002] Citrus huanglongbing is a serious disease with strong destructiveness and complex symptoms, making it difficult to diagnose in the field. Effective monitoring of the occurrence and distribution of citrus huanglongbing is the key to effective control of huanglongbing. Therefore, it is of great significance to seek more accurate, convenient, efficient and low-cost detection technology for the prevention and control of this disease. At present, PCR, nested PCR, real-time fluorescent quantitative PCR, loop-mediated isothermal amplification (L...

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Problems solved by technology

Although the above-mentioned detection techniques have high sensitivity, the operation steps are complicated and re

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