Cancer cell sorting and detecting device for specific binding breast cancer cell research and development

A breast cancer cell and detection device technology, which is applied in the field of cancer cell sorting and detection device specifically combined with breast cancer cell research and development, can solve the problems of sample liquid adhesion, large sample liquid loss, inability to adjust the sample liquid concentration, etc. The effect of increasing concentration, rapid concentration adjustment and simple structure

Pending Publication Date: 2022-01-18
JILIN UNIV FIRST HOSPITAL
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AI-Extracted Technical Summary

Problems solved by technology

[0004] However, in actual use, the device needs to adjust the concentration of the sample liquid through the cooperation of multiple components. More sample liquid adheres to the inner ...
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Method used

Further, the bottom outer wall of baffle plate 29 is fixedly connected with bristle, and the end of bristle away from baffle plate 29 contacts with the bottom inner wall of sorting tank 9, after the sorting detection of device to sample solution is completed, people need to When the device is cleaned, people can start the baffle plate 29 of the air pump 6 to rotate again, and the bottom inner wall of the sorting tank 9 can be cleaned by the rotation of the baffle plate 29 and the bristles, thereby improving the cleaning effect of the device.
Further, the detection assembly includes a detection plate 20 fixedly connected to the inner walls of the four sides of the housing 11, the detection plate 20 is located below the second mounting plate 19, the top of the detection plate 20 is provided with a detection groove 21, and the detection groove 21 is passed through the detection plate 20 Rapid detection of cancer cells in a sample solution is possible.
Further, the four side inner walls of the housing 11 are all fixedly connected with the second mounting plate 19, the top outer wall of the second mounting plate 19 is fixedly connected with the shielding plate 18, and the top of the second mounting plate 19 is provided with a drain tank 17. The drain tank 17 and the through hole 15 are located on the same vertical plane, and the shielding plate 18 provided at the same time can shield external signals, which can ensure that the sample solution is not interfered by external electromagnetic waves during the subsequent detection process, and improve detection accuracy.
Further, the positioning assembly includes fixed ears 2 fixedly connected to the outer walls of the four sides of the cover plate 1 and the housing 11, and the top of the outer walls of the four sides of the outer walls of the housing 11 is provided with threaded holes, located on the four sides of the cover plate 1 The top of the fixed ear 2 on the outer wall is provided with a jack, and the inner wall of the threaded hole is threaded with a fixed screw 3, and one end of the fixed screw 3 passes through the inside of the jack, and forms a threaded connection between the fixed screw 3 and the fixed ear 2. The tightness of the connection between the cover plate 1 and the casing 11 can be effectively ensured.
Further, the pressurized assembly includes a fixed seat 5 fixedly connected to the top outer wall of the cover plate 1, the top outer wall of the fixed seat 5 is fixedly connected with an air pump 6, and one side outer wall of the air pump 6 is plugged with a connecting pipe 4, and the connecting pipe One end of 4 communicates with the liquid inlet pipe 13, and the air pressure inside the sorting assembly can be adjusted by starting the air pump 6, thereby improving the sorting efficiency of the sample solution by the sorting assembly.
Further, the sorting assembly includes the first mounting plate 16 fixedly connected to the inner wall of the housing 11, the top outer wall of the first mounting plate 16 is fixedly connected with the sorting groove 9, and the cross section of the sorting groove 9 is a spiral , the liquid inlet pipe 13 is located directly above one end of the sorting tank 9, and one end of the sorting tank 9 is fixedly connected with a liquid separating tank 8 and a waste liquid tank 14, and the bottom inner wall of the waste liquid tank 14 is provided with a drain hole 28, and the sorting tank The inside of 9 is provided with a turbulence component. After the sample solution is added to the inside of the sorting tank 9, the cancer cells and white blood cells inside the sample solution are subjected to different sizes of inertial force and Dean force in the spiral sorting tank 9, so that Cancer cells and leukocytes are separated at one end of the spiral sorting tank 9. During this process, the cancer cell solution enters the inside of the liquid separating tank 8, while the white blood cell solution enters the waste liquid tank 14 on the other side and is discharged. The sorting tank 9 separates cancer cells and white blood cells, and not only has a simple structure, but also does not require human intervention during the sorting process, and the sorting...
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Abstract

The invention discloses a cancer cell sorting and detecting device for specific binding breast cancer cell research and development, and particularly relates to the technical field of cancer cell detection. The cancer cell sorting and detecting device comprises a cover plate and a shell arranged below the cover plate; a round hole is formed in the top of the cover plate; a liquid inlet pipe is fixedly connected to the circumferential inner wall of the round hole; a pressurizing assembly is arranged on the top of the cover plate; a sorting assembly and a detecting assembly are respectively arranged inside the shell; the outer wall of one side of the shell is fixedly connected with a control panel; the bottom of the sorting assembly is fixedly connected with a circulating assembly; and one end of the circulating assembly is communicated with the sorting assembly. According to the invention, cancer cell solution in a liquid separation tank can be pumped into a sorting tank again through a pump body for carrying out repeated sorting, so that the concentration of the cancer cell solution can be effectively improved, and the loss of the cancer cell solution can be effectively avoided in the process, and thus, the concentration of the cancer cell solution can be rapidly adjusted.

Application Domain

Technology Topic

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  • Cancer cell sorting and detecting device for specific binding breast cancer cell research and development
  • Cancer cell sorting and detecting device for specific binding breast cancer cell research and development
  • Cancer cell sorting and detecting device for specific binding breast cancer cell research and development

Examples

  • Experimental program(1)
  • Effect test(1)

Example Embodiment

[0031] Next, the technical solutions in the embodiments of the present invention will be described in connection with the drawings of the embodiments of the present invention, and it is understood that the described embodiments are merely the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art are in the range of the present invention without making creative labor premise.
[0032] Append Figure 1-8 One kind of the specific binding shown by the development of breast cancer cells and detecting cancer cell sorting apparatus 1 comprises a cover plate disposed in the housing 1 and the lower cover 11, the top cover defines a circular circumference of a circular hole an inner wall fixedly connected with a liquid inlet pipe 13, the top cover 1 is provided with a pressurizing device, the interior of the housing 11 are provided with sorting and detection component assembly, the outer wall of one side of the housing 11 is fixedly connected with a control panel 7;
[0033]Sorting assembly bottom circulation assembly is fixedly connected, at one end of the circulation unit and sorting components communicate with each other, the cancer cells can be separated by the sorting assembly leukocytes, while sub-component selected from not only a simple structure and without manual sorting process intervention, high separation efficiency to meet people's needs.
[0034] Further, the pressurizing assembly comprising a top cover fixedly attached to the outer wall of the fixing base 5, the top of the outer wall 5 of the holder 6 is fixedly connected with a pump, a side of the outer wall of the pump 6 has one end connected to the plug pipe 4, the connection pipe 4 communication with the liquid inlet pipe 13 to each other, the air pressure regulator 6 can be sorted by starting the internal pump components, thereby improving the separation efficiency of the separation component of the sample solution.
[0035] Further, the top of the outer wall of the housing 11 defines a seal slot 10, the bottom of the outer wall of the cover 1 is fixedly connected with the sealing strip 12 cards, seal cards size pieces of specifications of the seal groove 12 of the adapter 10 with four housing 11 outer walls are fixedly connected with a positioning assembly, by sealing the clip strip provided between cooperating and sealing groove 1012, the entire apparatus can effectively guarantee the tightness and hygiene.
[0036] Further, the positioning assembly comprising a fastening lug fixedly connected to the cover plate 11 and the housing 1 of the four sides of the outer wall 2, four sides of the outer wall of the housing 11 located in the top of the fixed lugs 2 defines a threaded hole located in the cover plate 1 is fixed on four sides of the outer wall 2 defines the top of the ear jack, the inner wall of the circumferential thread of the threaded hole 3 fixing bolt is connected to one end of the set screw 3 through the insertion hole from the inside, is formed by fixing lugs fixed between the screw 3 and the screw 2 is connected, can effectively 1 to ensure the tightness between the cap plate 11 and the connecting housing.
[0037] Further, the sorting assembly includes a fixed inner wall of the connector housing 11 in the first mounting plate 16, the top of the first mounting plate 16 is fixedly connected to the outer wall of the groove 9 with sorting, sorting cross section of the helical groove 9, into the liquid 13 is located directly above the tube 9 points at one end of the groove is selected from one end of the sorting groove 9 is fixedly connected watched tank 8 and a waste liquid tank 14, the inner bottom wall of the waste liquid tank 14 defines a drain hole 28, the groove 9 of the internal separation after the assembly is provided with a spoiler, added to the interior of the sorting groove 9 in the sample solution, the sample solution inside the cancer cells and white blood cells of different sizes by the inertial force and the force in the Dean sorting spiral groove 9, so that the cancer cells leukocytes are separated at one end of the helical groove 9 of the sorting, the cancer cells into the interior of the solution tank 8 points in this process, leukocytes was discharged into another 14 waste tank side, by using the spiral groove sorting 9 pairs cancer cells and leukocyte separation, is not only simple structure, while the sorting process without human intervention, high separation efficiency to meet people's needs.
[0038] Further, the catheter assembly includes loop fixedly attached to the bottom of the outer wall 16 of the first mounting plate 22, the inner bottom wall of the sub tank 8 defines a through hole 15, the catheter 22 communicates with the through hole 15, the catheter 22 the outer wall is fixedly connected to one side of the first control valve 23, the catheter 22 side plug 24 has a suction catheter, suction catheter 24 away from the catheter insertion end 22 of the pump body 25, the pump 25 side of the plug has a second control valve 27 is fixed to the tube 26, fixed to one end of the tube 26 extends to the interior of the sorting groove 9, the side of the outer wall of the fixed pipe 26 is provided, when the sample solution is selected from the completed assembly after the division by the sorting, staff can close the catheter 22 side of the first control valve 23, the pump 25 starts and then the staff can be divided inside the solution tank 8 cancer cell sorting channels again drawn into the interior 9 through the pump over 25 secondary sorting, thereby effectively increasing the concentration of the solution of cancer cells, in this process can effectively avoid the loss of solution cancer, cancer cells can be quickly adjusted solution concentration, to meet people's needs.
[0039] Further, four side wall of the housing 11 are fixedly connected to the second mounting plate 19, the top of the second mounting plate 19 fixedly connected to the outer wall of the shield plate 18, the top of the second mounting plate 19 defines a discharge tank 17, the discharge tank 17 and through hole 15 in the same vertical plane, while the shielding plate 18 may be disposed outside of the mask signal, to ensure that the sample solution is not outside electromagnetic interference in the subsequent detection process, improve the detection accuracy.
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Description & Claims & Application Information

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the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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