Quality detection method of asarum or asarum extract for treating migraine

Abstract

The invention belongs to the technical field of quality detection of medicinal materials, and particularly relates to a quality detection method of asarum or asarum extract for treating migraine. The invention researches the material basis of the traditional Chinese medicinal material asarum for treating migraine through a network pharmacology method, and provides a method for detecting the quality of asarum or asarum extract by detecting the content of higenamine and cherry glycoside in asarum or asarum extract based on the material basis. By utilizing the quality detection method disclosed by the invention, the asarum extract, the asarum medicinal material or the asarum processed product with a better treatment effect on migraine can be effectively selected, and the quality detection method has a good prospect in clinical application.

Description

technical field

[0001] The invention belongs to the technical field of quality detection of medicinal materials, and in particular relates to a quality detection method of Asarum or Asarum extract used for treating migraine. Background technique

[0002] In order to better store or use Chinese herbal medicines, people usually process them. For example, the commonly used processing methods of Chinese medicinal herb Asarum include frying, honey, vinegar, licorice, wine, rice swill, salt, ginger, alkali and alkali vinegar, etc. In processed Chinese medicinal materials, the type and content of active substances usually change, resulting in changes in their efficacy for various applicable diseases. On the other hand, the place of origin and growth status of the medicinal material itself will also significantly affect its efficacy.

[0003] Therefore, in order to make better use of Chinese herbal medicines, it is very important to understand the active substances in the medicine...

Examples

Embodiment 1

[0029] Example 1 Prediction of Asarum anti-migraine material basis

[0030] 1. Database and software

[0031] The database and software used in this embodiment are shown in the following table:

[0032] Table 1 Database information

[0033]

[0034]

[0035] Table 2 Analysis software information

[0036]

[0037]

[0038] 2. Experimental method

[0039] 2.1. Establishment of Asarum component database and screening of active components

[0040] Through China National Knowledge Network, Wanfang Data and Baidu Academic Retrieval of existing literature, combined with public databases SymMap, TCMSP and ETCM, the chemical components contained in Asarum were collected and unique values ​​were retained to establish a database of components of Asarum; PubChem was used to find the PubChem of each compound CID, 3D structure and Smiles; Import the Smiles formula of the chemical composition into FAF-Drugs4 to conduct ADMET evaluation and screening of all compounds, set "Fil...

Embodiment 2

[0066] Example 2 Pharmacodynamic verification of asarum anti-migraine substances

[0067] 1. Experimental materials

[0068] 1.1. Experimental drugs

[0069] Asarum: purchased from the professional market of traditional Chinese medicinal materials on Jiefang Road, Chongqing, and was identified as the dried root and rhizome of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. Pulverizer pulverizes, crosses No. 5 sieves (80 order), gets asarum powder. Prepare the required concentration of liquid medicine with distilled water before use.

[0070] 1.2. Experimental animals

[0071] 90 healthy male SD rats, weighing 200±20g, were purchased from Chengdu Dashuo Experimental Animal Co., Ltd., license number SCXK (Sichuan) 2015-030. Adaptive feeding for a period of time before the experiment. Raising conditions for experimental animals: indoor temperature 20-26°C, relative humidity 40%-70%, 12 hours in the daytime, 12 hours in the night, free to eat and drink. ...

Embodiment 3

[0125] Example 3 Comparison of Higenamine and Prunin Contents in Different Processed Products of Herba Asarum

[0126] 1. Experimental materials

[0127] 1.1. Experimental medicinal materials

[0128] Asarum: purchased from the professional market of traditional Chinese medicinal materials on Jiefang Road, Chongqing, identified as the dried root of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. and rhizomes.

[0129] 1.2. Experimental Instruments

[0130] Table 11 Instrument information

[0131]

[0132] 1.3. Experimental reagents

[0133] Table 12 Reagent Information

[0134]

[0135] 2. Experimental method

[0136] 2.1. Processing of different processed products of Asarum

[0137] 2.1.1 Purification and cutting

[0138] According to the requirements of the "Chinese Pharmacopoeia" 2020 edition, the Asarum medicinal material was cut into sections to remove impurities.

[0139] 2.1.2 Processing method

[0140] According to literature review...

technical field

[0001] The invention belongs to the technical field of quality detection of medicinal materials, and in particular relates to a quality detection method of Asarum or Asarum extract used for treating migraine. Background technique

[0002] In order to better store or use Chinese herbal medicines, people usually process them. For example, the commonly used processing methods of Chinese medicinal herb Asarum include frying, honey, vinegar, licorice, wine, rice swill, salt, ginger, alkali and alkali vinegar, etc. In processed Chinese medicinal materials, the type and content of active substances usually change, resulting in changes in their efficacy for various applicable diseases. On the other hand, the place of origin and growth status of the medicinal material itself will also significantly affect its efficacy.

[0003] Therefore, in order to make better use of Chinese herbal medicines, it is very important to understand the active substances in the medicine...

Examples

Embodiment 1

[0029] Example 1 Prediction of Asarum anti-migraine material basis

[0030] 1. Database and software

[0031] The database and software used in this embodiment are shown in the following table:

[0032] Table 1 Database information

[0033]

[0034]

[0035] Table 2 Analysis software information

[0036]

[0037]

[0038] 2. Experimental method

[0039] 2.1. Establishment of Asarum component database and screening of active components

[0040] Through China National Knowledge Network, Wanfang Data and Baidu Academic Retrieval of existing literature, combined with public databases SymMap, TCMSP and ETCM, the chemical components contained in Asarum were collected and unique values ​​were retained to establish a database of components of Asarum; PubChem was used to find the PubChem of each compound CID, 3D structure and Smiles; Import the Smiles formula of the chemical composition into FAF-Drugs4 to conduct ADMET evaluation and screening of all compounds, set "Fil...

Embodiment 2

[0066] Example 2 Pharmacodynamic verification of asarum anti-migraine substances

[0067] 1. Experimental materials

[0068] 1.1. Experimental drugs

[0069] Asarum: purchased from the professional market of traditional Chinese medicinal materials on Jiefang Road, Chongqing, and was identified as the dried root and rhizome of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. Pulverizer pulverizes, crosses No. 5 sieves (80 order), gets asarum powder. Prepare the required concentration of liquid medicine with distilled water before use.

[0070] 1.2. Experimental animals

[0071] 90 healthy male SD rats, weighing 200±20g, were purchased from Chengdu Dashuo Experimental Animal Co., Ltd., license number SCXK (Sichuan) 2015-030. Adaptive feeding for a period of time before the experiment. Raising conditions for experimental animals: indoor temperature 20-26°C, relative humidity 40%-70%, 12 hours in the daytime, 12 hours in the night, free to eat and drink. ...

Embodiment 3

[0125] Example 3 Comparison of Higenamine and Prunin Contents in Different Processed Products of Herba Asarum

[0126] 1. Experimental materials

[0127] 1.1. Experimental medicinal materials

[0128] Asarum: purchased from the professional market of traditional Chinese medicinal materials on Jiefang Road, Chongqing, identified as the dried root of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. and rhizomes.

[0129] 1.2. Experimental Instruments

[0130] Table 11 Instrument information

[0131]

[0132] 1.3. Experimental reagents

[0133] Table 12 Reagent Information

[0134]

[0135] 2. Experimental method

[0136] 2.1. Processing of different processed products of Asarum

[0137] 2.1.1 Purification and cutting

[0138] According to the requirements of the "Chinese Pharmacopoeia" 2020 edition, the Asarum medicinal material was cut into sections to remove impurities.

[0139] 2.1.2 Processing method

[0140] According to literature review...

Images