Application of Lactobacillus sake and stachyose composition in the preparation of constipation treatment medicine
A technology of Lactobacillus sake, composition, applied in the direction of bacteria, Lactobacillus, application, etc. used in food preparation
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Embodiment 1
[0039] Example 1. Isolation, screening and identification of lactic acid bacteria
[0040] 1.1 Isolation of Lactobacillus sake furu2019
[0041] 1) Prepare MRS agar medium after autoclaving at 121°C for 15min, then cool it to 50°C, shake it gently, and pour it into a petri dish after mixing;
[0042] 2) Dissolve 1g of homemade fermented bean curd sample in 5ml of 1xPBS, and then take out 100ul of it and spread it on MRS medium;
[0043] 3) Incubate for 48h at 37℃, 0.5% CO2 environment;
[0044] 4) Pick wet, circular raised gray-white single colonies for transfer.
[0045] 1.2 Culture preservation
[0046] The laboratory uses the MRS medium containing 30% glycerol as the bacterial preservation solution for the cryopreservation of the strains. The methods are as follows:
[0047] 1) Sterilize the 2mL sterilization tube at 121℃ for 15min and prepare it for use;
[0048] 2) After the bacteria are continuously transferred on the MRS medium for 3 times, add 2ml of sterile bacteri...
Embodiment 2
[0062] Example 2. Evaluation of constipation improvement function of Lactobacillus sake furu2019
[0063] 1. Methods and evaluation indicators
[0064] 1. Animal experiment: 40 SPF grade 6-8 week old male ICR mice (24-26 g) were randomly divided into five groups (eight mice in each group) after adaptive feeding for seven days: blank control group, constipation model group, stachyose (2 kg per pack, provided by China Institute of Food and Fermentation Industry) intervention group (ST), Lactobacillus sake intervention group (LS), stachyose + Lactobacillus sake mixed intervention group (ST+LS). The blank control group and the model group were given 0.3 ml of PBS every day, the stachyose intervention group was given 0.3 ml of stachyose solution (the dose was 1.5 g / kg, about 35 mg per mouse), and the Lactobacillus sake intervention The group was given 0.3 ml of Lactobacillus sake (dose of 3.5 × 10 9 CFU), stachyose + Lactobacillus sake mixed intervention group was given 0.3 ml of...
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