Application of Lactobacillus sake and stachyose composition in the preparation of constipation treatment medicine

A technology of Lactobacillus sake, composition, applied in the direction of bacteria, Lactobacillus, application, etc. used in food preparation

Active Publication Date: 2022-07-22
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, drug treatment often has many side effects

Method used

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  • Application of Lactobacillus sake and stachyose composition in the preparation of constipation treatment medicine
  • Application of Lactobacillus sake and stachyose composition in the preparation of constipation treatment medicine
  • Application of Lactobacillus sake and stachyose composition in the preparation of constipation treatment medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Isolation, screening and identification of lactic acid bacteria

[0040] 1.1 Isolation of Lactobacillus sake furu2019

[0041] 1) Prepare MRS agar medium after autoclaving at 121°C for 15min, then cool it to 50°C, shake it gently, and pour it into a petri dish after mixing;

[0042] 2) Dissolve 1g of homemade fermented bean curd sample in 5ml of 1xPBS, and then take out 100ul of it and spread it on MRS medium;

[0043] 3) Incubate for 48h at 37℃, 0.5% CO2 environment;

[0044] 4) Pick wet, circular raised gray-white single colonies for transfer.

[0045] 1.2 Culture preservation

[0046] The laboratory uses the MRS medium containing 30% glycerol as the bacterial preservation solution for the cryopreservation of the strains. The methods are as follows:

[0047] 1) Sterilize the 2mL sterilization tube at 121℃ for 15min and prepare it for use;

[0048] 2) After the bacteria are continuously transferred on the MRS medium for 3 times, add 2ml of sterile bacteri...

Embodiment 2

[0062] Example 2. Evaluation of constipation improvement function of Lactobacillus sake furu2019

[0063] 1. Methods and evaluation indicators

[0064] 1. Animal experiment: 40 SPF grade 6-8 week old male ICR mice (24-26 g) were randomly divided into five groups (eight mice in each group) after adaptive feeding for seven days: blank control group, constipation model group, stachyose (2 kg per pack, provided by China Institute of Food and Fermentation Industry) intervention group (ST), Lactobacillus sake intervention group (LS), stachyose + Lactobacillus sake mixed intervention group (ST+LS). The blank control group and the model group were given 0.3 ml of PBS every day, the stachyose intervention group was given 0.3 ml of stachyose solution (the dose was 1.5 g / kg, about 35 mg per mouse), and the Lactobacillus sake intervention The group was given 0.3 ml of Lactobacillus sake (dose of 3.5 × 10 9 CFU), stachyose + Lactobacillus sake mixed intervention group was given 0.3 ml of...

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Abstract

The invention discloses a Lactobacillus sakei strain, the preservation number of the strain is CGMCC NO.22254, the preservation date is April 28, 2021, the preservation classification is named Lactobacillus sakei (Lactobacillus sake), and the preservation unit is China General Microbiology Center of the Microorganism Culture Collection Management Committee. The sequence of the 16S rDNA of the strain is shown in SEQ ID NO: 1, and the present invention also discloses the application of the strain and the combination of the strain and stachyose in the preparation of food, health care products and medicines for improving digestive tract function, The strain is harmless to animals and has the effect of regulating and improving constipation.

Description

technical field [0001] The invention relates to a probiotic and its combined application with a prebiotic, belonging to the field of medicine and health food science. Background technique [0002] Slow transit constipation (STC) is a common type of constipation, and its clinical symptoms include hard and dry stools, difficulty defecation, and infrequent defecation. Usually, constipation is mainly concentrated in the elderly, women and people with high stress. In parts of Africa, the incidence of constipation is as high as 30%, and the main mechanism of STC is prolonged intestinal transit time, prolonged bacterial fermentation time, and prolonged water absorption time due to the slowing of intestinal peristalsis. [0003] The pathogenesis of constipation is more complex. The mechanisms of relieving constipation mainly include stimulating intestinal peristalsis by regulating intestinal flora by competition and producing short-chain fatty acids; regulating intestinal peristal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61K31/702A61P1/10A23L33/135C12R1/225
CPCA61K35/747A61K31/702A61P1/10A23L33/135A23V2002/00A23V2400/179A61K2300/00A23V2200/3204Y02A50/30
Inventor 任志鸿宋利琼肖玉春黄元铭
Owner ICDC CHINA CDC
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