The invention discloses a preparation method of sugar-isolated conditioning and supplementing collagen beverage. The preparation method comprises the following steps: step 1, preparing the following raw materials: 4% of collagen peptide, 5% of resistant dextrin, 5% of passion fruit concentrated juice, 5% of apple concentrated juice, 5% of stachyose, 4% of elastin peptide, 4% of haematococcus pluvialis, 3% of erythritol, 4% of sugar alcohol, 3% of L-ascorbic acid, 6% of L-arabinose; 5% of hawthorn powder, 5% of houttuynia cordata, 5% of instant green tea powder, 4% of olive fruit powder, 6% ofwhite kidney bean extract, 6% of grape seed extract, 5% of black ginger, 4% of asparagus powder, 5% of citric acid, 5% of sodium citrate and 1% of edible essence; step 2, feeding and stirring; step 3,filtering; step 4, sterilization treatment; step 5, detection; step 6, homogenizing; and step 7, filling. The beverage has functions of blocking decomposition and absorption of starch and sucrose, controlling rise of blood sugar, and blocking generation of AGEs; promoting metabolism of AGEs, adjusting the intestines, brightening complexion, and delaying aging; supplementing collagen peptides, supplementing elastin peptides, preventing collagen loss, promoting collagen synthesis and the like.