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Reagent kit and use based on antigen-antibody reaction

An antigen-antibody and kit technology, which is applied in the direction of chemical reaction of materials for analysis, biological testing, and material analysis by observing the influence of chemical indicators The number of detectors is reduced, the detection time is reduced and the detection comparability is improved.

Inactive Publication Date: 2004-09-01
CHENGDU KUACHANG SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] All in all, at present, in the above-mentioned kits, only antigens or antibodies are immobilized in a reactor on the probe board, and the labeled substances contained in the labeling system are either anti-antibodies, specific antibodies, or specific antigens. Therefore, a set of reactants in the above kit (one reactor plus labeling system and all materials used in one reactor in the auxiliary part) cannot be used for the simultaneous determination of antigen and antibody in one sample

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] Example 1, preparation of rapid screening kit for blood donors

[0016]The kit in this example can be used for rapid screening of blood donors. The production of the kit probe board is to mix the HBsAg-immunized rabbit anti-HBsAg, HCV fusion antigen, HIV fusion antigen, and syphilis fusion antigen according to the optimal concentration and ratio, and then coat it on a 96-well microtiter plate. Bovine serum albumin blocked and then dried. The labeling system of the kit is composed of markers and substrates (substrate chromogenic solutions A and B). The markers use antigens and antibodies that are different from the coated antigen antibody and paired with the coated antigen antibody: anti-HBsAg, HCV Fusion protein antigen of HIV1+2, syphilis antigen. These antigens and antibodies used in the labeling system are enzyme-labeled separately or mixed at a determined concentration and ratio, and then used for later use. The auxiliary parts of the kit in this example include ...

example 2

[0017] Example 2, the preparation example of blood transfusion chip kit

[0018] In this example, the preparation of the probe board of the kit uses the activated glass slide as the carrier, and the probes used include anti-HBsAg, HCV antigen, HIV1+2 antigen, syphilis antigen, HTLV1+2 antigen, manual spotting, each antigen Point 6 sample points with the determined optimal concentration, each sample point diameter is 200um. After spotting, the blank carrier surface was blocked with bovine serum albumin and dried. The labeling system of the kit in this example uses antigens and antibodies that are different from the original coated antigen antibody paired with the coated antigen antibody: anti-HBsAg, fusion protein antigen of HCV, fusion protein antigen of HIV1+2, syphilis antigen , HTLV1+2 antigen. These antigens and antibodies are labeled with rhodamine at the determined optimal concentration and ratio for later use, or these antigens and antibodies can be respectively label...

example 3

[0019] Example 3: Application of Rapid Screening Kit for Blood Donors

[0020] This example uses the 96-well plate kit prepared in Example 1, with 3 wells of negative control, 2 wells of positive control, 1 well of blank control, and 8 known test samples (the positive and negative antigen-antibody reactions have been determined by ELISA method, see table one). During the test, except for the blank, add diluent, negative and positive controls and tested samples according to different wells. After the reaction is completed, add enzyme-labeled paired antigen and antibody, chromogenic reagent, and stop solution. Then use microplate reader to measure, and its result is as follows:

[0021] Table 1. Rapid Screening Results of Blood Donors

[0022] Sample HBsAg HCV HIV Syphilis HTLV This kit

[0023] 1 + - - - - 0.249

[0024] 2 - + - - - 0.253

[0025] 3 - - + - - 0.328

[0026] 4 - - - + - 0.225

[0027] 5 - - - - + 0.214

[0028] 6 + + - - - 0.265

[0029] 7 - - + + - 0.3...

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Abstract

A novel reagent kit based on antigen-antibody reaction is characterized by that both antigen and antibody are fixed in a same selective reactor on probe plate, its mark system can mark both target antigen and target antibody, and a set of reactants is only used to quantitatively and qualitatively detect different antigens and antibodies in a specimen. Its advantages are high speed and efficiency and low cost.

Description

technical field [0001] The present invention relates to a reagent kit based on antigen-antibody reaction, especially a reagent kit in which both antigen probe and antibody probe are immobilized in one reactor, which is used to characterize and / or identify target antibody and target antigen in the same sample. or kits for quantitative detection. [0002] The present invention also relates to the production method and application of the kit of the present invention. Background technique [0003] The so-called kit in the present invention refers to an essential consumable in some qualitative and / or quantitative detection, which is used to react with the target object and express the reaction result in a determinable manner. It is a probe card containing a selective reactor, the important part is a labeling system containing a marker, and there are auxiliary parts (such as a buffer, etc.). The so-called selective reactor in the present invention refers to a fixed place where p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N21/76G01N21/78G01N33/543G01N37/00
CPCG01N33/54366G01N33/53G01N33/543
Inventor 邹方霖陈春生胡劲梅王建霞
Owner CHENGDU KUACHANG SCI & TECH CO LTD