Use of bifidobacterium cell wall and bifidobacterium cell wall protein in pharmacy

A technology of bifidobacteria cells and proteins is applied in the pharmaceutical field to achieve the effects of effective growth advantages, wide sources and simple preparation methods

Inactive Publication Date: 2005-01-12
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Studies have shown that it has an important impact on the body's immune system, especially the activation of the body's immune system, for example, activation of macrophages, B lymphocytes, NK cells, ... (see "Immune activation of bifidobacteria and their biological Scientific Significance”, Wang Lisheng Review, Pan Lingjia Zhou Dianyuan Review, Foreign Medical Physiology, Pathology and Clinical Volume, 1998.18(4), P 320-322 ), but there is no report on the activation of defensins by bifidobacteria cell walls and cell wall proteins

Method used

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  • Use of bifidobacterium cell wall and bifidobacterium cell wall protein in pharmacy
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  • Use of bifidobacterium cell wall and bifidobacterium cell wall protein in pharmacy

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Embodiment Construction

[0023] 1. Cell lines and bacterial strains

[0024] The human intestinal glandular epithelial cell line HT-29 is derived from ATCC and preserved by the applicant’s laboratory of infection and immunity. Laboratory culture, and by the National Institute of Pharmaceutical and Biological Products and the Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Sciences identified. Inoculate in MRS medium and culture anaerobically at 37°C for 48 hours.

[0025] Prepare MRS medium (1 liter):

[0026] Peptone 10g, yeast powder 5g, tryptone 3g, glucose 10g, soluble starch 0.5g, ferrous sulfate 100mg, manganese sulfate 6.74mg, magnesium sulfate 200mg, cysteine ​​0.5g, potassium dihydrogen phosphate 1g, hydrogen phosphate Dipotassium 1g, sodium chloride 100mg, sodium acetate 5g, trisodium citrate 2g, Tween-80 1.0g, add distilled water to 1 liter, adjust pH to 7.2, high temperature, high pressure, sterilization.

[0027] 2. Reagents

[0028]Agarose, two-step RT-PCR k...

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Abstract

The present invention utilizes the tests to show that the bifidobacterium cell wall and cell wall protein area effective factor for activating phylaxin gene, can obviously raised the expression of the phylaxin, so that the bifidobacterium cell wall and cell wall protein can be used for preparing the medicine for activating the phylaxin gene of mammals. Its medication method can utilize per os, intraveously (IV), inhalation or suppository, enteroclyster or oral irrigation. According to the characteristics of phylaxin of human body said invented medicine mainly is used for immunotherapy of infective diseases of digestive tract, respiratory tract and urinogenital tract, etc.

Description

1. Technical field [0001] The present invention relates to the use of bifidobacterium cell wall and bifidobacterium cell wall protein, especially in the field of pharmacy. 2. Background technology [0002] Bifidobacterium is one of the earliest physiological bacteria discovered, and has been clinically applied in the prevention and treatment of intestinal diseases and the treatment of liver diseases (see "Bifidobacteria" p 131-139 , edited by Kang Bai, Dalian Maritime University Press, May 1998). Studies have shown that it has an important impact on the body's immune system, especially the activation of the body's immune system, for example, activation of macrophages, B lymphocytes, NK cells, ... (see "Immune activation of bifidobacteria and their biological Scientific Significance”, Wang Lisheng Review, Pan Lingjia Zhou Dianyuan Review, Foreign Medical Physiology, Pathology and Clinical Volume, 1998.18(4), P 320-322 ), but there is no report on the bifidobacteria cell wal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P31/00A61P37/02
Inventor 王伯瑶王国兴黄宁吴琦汪宇辉冯云
Owner SICHUAN UNIV
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