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Recompounded microbe germicide for prevrnting and controlling banded sclerotial blight and green smut of rice

A technology of rice sheath blight and microorganisms, applied in the fields of application, biocide, animal repellent, etc., can solve the problems of no high-efficiency Bacillus subtilis, no microbial preparations, microbial preparations and chemical pesticides, etc. Achieve rapid antibacterial effect, reduce the incidence of disease, and have strong colonization ability

Inactive Publication Date: 2005-02-09
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no microbial preparations with high-efficiency Bacillus subtilis, nor are there any microbial preparations or compound preparations of microbial preparations and chemical pesticides that are widely used in farmland to prevent and control rice diseases

Method used

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  • Recompounded microbe germicide for prevrnting and controlling banded sclerotial blight and green smut of rice
  • Recompounded microbe germicide for prevrnting and controlling banded sclerotial blight and green smut of rice

Examples

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Embodiment 1

[0020] Implementation example 1, Bacillus subtilis CGMCC0808 fermentation broth and Jinggangmycin compounded into the product of the present invention: the spore content is greater than or equal to 90% of the thalline and the bacterium content is greater than or equal to 2 × 10 10 cfu / ml Bacillus subtilis CGMCC0808 fermentation broth and 5% Jinggangmycin are compounded according to the volume ratio of 1:1, and stirred evenly to obtain the microbial compounded pesticide product (wenquning) of the present invention. A benzoic acid preservative accounting for 2‰ of the total volume can be added to the above-mentioned microbial compound pesticide to prevent the agent from spoiling. After adding the benzoic acid preservative, stir evenly, and then pack.

Embodiment 2

[0021] Implementation example 2, the industrial production method of microbial composite pesticide product (ventroning) of the present invention: at first according to routine method, in laboratory, bacillus subtilis bacterial strain CGMCC0808 is activated, then carries out bacterial strain cultivation according to conventional method in laboratory, activates The resulting strain was transferred into 500ml of YPG (5g of tryptone, 5g of yeast extract, 5g of glucose, and 1000ml of water) culture medium, and after cultivating at 120 rpm and 28°C for 48 hours, the inoculum size was 1:150-1:300. The cultured strains are moved into the seed tank (temperature 28-32°C, pH value 7.0-7.4) for fermentation and cultivation, the fermentation time is 18-48 hours, during which the seed tank is tested every 2 hours, when the bacteria content greater than or equal to 10 10 For cfu / ml, according to the inoculum size 1:150-1:300, transfer the fermented liquid in the seed tank to a large fermente...

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Abstract

The present invention is recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice. The recompounded microbe germicide is recompounded through thefermentation culture of Bacillus subtilis and recompounding the fermented liquid with certain content of Bacillus subtilis and Jinggangmeisu. It is used in preventing and controlling banded sclerotial blight and green smut of rice in field via utilizing the complementary superiority of Bacillus subtilis and Jinggangmeisu. It has high functions of preventing and controlling banded sclerotial blight and green smut of rice, less influence on rice quality, stable germicidal effect and long preservation period.

Description

(1) Technical field [0001] The invention relates to a microbial compound pesticide, in particular to a microbial compound pesticide capable of antagonizing rice sheath blight and rice smut. (2) Background technology [0002] Rice sheath blight and rice false smut are the main diseases in rice production. Rice sheath blight occurs in major rice-growing areas in the United States, South America, Europe, and Africa. The disease is more severe in South China, Central China and East China, and also occurs in North China, Northeast China and Yunnan rice fields, and it is more serious in some areas. Generally, the production of the disease is reduced by 5-10%, and the production of severe fields is reduced by 50-70%. Rice false smut occurs in all rice-growing regions in my country, and rice false smut can cause yield loss, and more seriously, affect rice quality. It is reported that rice smut disease grains are highly toxic to humans, and feeding livestock and poultry with diseas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02
Inventor 陈志谊刘永锋
Owner JIANGSU ACAD OF AGRI SCI
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