Process for establishing hepatitis animal model induced by natural killing cell

A technology of natural killer cells and animal models, which is applied in the fields of liver immunology and experimental zoology, can solve the problems that NK cell pathogenic mechanism has not made great progress and is cumbersome, and achieves obvious clinical symptoms, good repeatability, and operation methods. convenient effect

Active Publication Date: 2006-04-26
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology

[0003] Although the American "Journal of Immunology" (Journal of Immunology, 1983; 131: 1531-1538) and the American "Journal of Leukocyte Biology" (Journal of Leukocyte Biology, 2004; 76: 743-59) respectively reported that NK cells in mice Cytomegalovirus (MCMV) and hepatitis C virus (HCV) infection play an important role in liver parenchymal cell injury, but due to the lack of human liver samples and the cumbersome establishment of MCMV and HCV infection models, safety factors and other limitations, especially Due to the lack of a suitable model of NK cell-mediated hepatitis, the detailed pathogenic mechanism of NK cells in liver injury has not been greatly developed
Therefore, in order to better and more conveniently study the role of NK cells in the immune injury of hepatitis, as well as to screen valuable liver-protecting drugs and observe the efficacy, it is necessary to establish an animal model of hepatitis mediated by NK cells, but so far no There are reports about the hepatitis model mediated by NK cells

Method used

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Embodiment 1

[0021] The present embodiment establishes the method for the hepatitis model mediated by NK cells, comprising the following steps:

[0022] 1. Preparation of different pure-line mice: select at least one group of BALB / c pure-line mice, one group of C57BL / 6J pure-line mice, one group of pure-line BALB / c background SCID mice, one group of pure-line BALB A group of nude mice on a BALB / c background, a group of CD1d-deficient mice on a clonal BALB / c background, and a group of NK cell-depleted C57BL / 6J clonal mice. In addition to comparing the effect of Poly IC on liver damage in mice of different ages, the mice were all 6-8 weeks old, because the symptoms of the model established at this age were stable, and it was easy to simulate human disease conditions; in addition to doing gender difference comparison experiments, All other experiments used female mice; the mice were clean (SPF grade); they were kept in a pathogen-free animal room with special care, and the feed and water were...

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Abstract

The method for establishing a hepatitis model mediated by natural killer cells (NK cells) of the present invention is characterized in that a virus mimic Poly IC (Poly IC) is selected for 7.5-20 micrograms per gram of body weight, and injected intraperitoneally or intravenously In 6-8 week-old pure-line BALB / c or C57BL / 6J mice, serum alanine aminotransferase and aspartate aminotransferase were significantly elevated 12-24 hours after Poly IC injection, and hepatocytes appeared in liver histology Punctate necrosis, local inflammatory cell infiltration, increased proportion and absolute number of NK cells in the liver. The establishment of this model provides a powerful tool for systematically studying the pathogenesis of hepatitis, screening valuable liver-protecting drugs and observing the curative effect.

Description

Technical field: [0001] The invention belongs to the technical field of liver immunology and experimental zoology, and in particular relates to a method for establishing an animal model of hepatitis mediated by natural killer cells (NK cells). Background technique: [0002] Hepatitis is a disease that seriously endangers people's life and health and has a high mortality rate. The etiology mainly includes the destruction of liver parenchymal cells caused by viral infection, excessive alcohol intake and ingestion of chemical poisons. Its pathogenic mechanism is complex. Among them, liver immune injury has attracted much attention, especially the pathological injury involving hepatic lymphocytes has become a crucial event in the pathogenesis of various hepatitis. Studying how lymphocytes participate in liver injury will help to understand the pathogenic mechanism of hepatitis, and will promote the prevention and treatment of liver diseases. Due to the lack of human liver sampl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/06
Inventor 田志刚董忠军魏海明
Owner UNIV OF SCI & TECH OF CHINA
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