Process for establishing hepatitis animal model induced by natural killing cell

Abstract

The method for establishing a hepatitis model mediated by natural killer cells (NK cells) of the present invention is characterized in that a virus mimic Poly IC (Poly IC) is selected for 7.5-20 micrograms per gram of body weight, and injected intraperitoneally or intravenously In 6-8 week-old pure-line BALB / c or C57BL / 6J mice, serum alanine aminotransferase and aspartate aminotransferase were significantly elevated 12-24 hours after Poly IC injection, and hepatocytes appeared in liver histology Punctate necrosis, local inflammatory cell infiltration, increased proportion and absolute number of NK cells in the liver. The establishment of this model provides a powerful tool for systematically studying the pathogenesis of hepatitis, screening valuable liver-protecting drugs and observing the curative effect.

Description

Technical field: [0001] The invention belongs to the technical field of liver immunology and experimental zoology, and in particular relates to a method for establishing an animal model of hepatitis mediated by natural killer cells (NK cells). Background technique: [0002] Hepatitis is a disease that seriously endangers people's life and health and has a high mortality rate. The etiology mainly includes the destruction of liver parenchymal cells caused by viral infection, excessive alcohol intake and ingestion of chemical poisons. Its pathogenic mechanism is complex. Among them, liver immune injury has attracted much attention, especially the pathological injury involving hepatic lymphocytes has become a crucial event in the pathogenesis of various hepatitis. Studying how lymphocytes participate in liver injury will help to understand the pathogenic mechanism of hepatitis, and will promote the prevention and treatment of liver diseases. Due to the lack of human liver sampl...

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Problems solved by technology

[0003] Although the American "Journal of Immunology" (Journal of Immunology, 1983; 131: 1531-1538) and the American "Journal of Leukocyte Biology" (Journal of Leukocyte Biology, 2004; 76: 743-59) respectively reported that NK cells in mice Cytomegalovirus (MCMV) and hepatitis C virus (HCV) infection play an important role in liver parenchymal cell injury, but due to the lack of human liver samples and the cumbersome establishment of MCMV and HCV infection models, safety factors and other limitations, especially Due to the lack of a suitable model of NK cell-mediated hepatitis, the detailed pathogenic mechanism of NK cells in liver injury has not been greatly developed

Therefore, in order to better and more conveniently study the role of NK cells in the immune injury of hepatitis, as well as to screen valuable liver-protecting drugs and observe the efficacy, it is necessary to establish an animal model of hepatitis mediated by NK cells, but so far no There are reports about the hepatitis model mediated by NK cells