40results about How to "Safe method of operation" patented technology

The invention discloses a method and device for marking plant carbon isotopes. The method comprises following steps: sowing seed in the cultivating disk which located in the soil; putting cultivating disk into air- tight box; adding sodium carbonate marked with carbon-14 or carbon- 13 under air- tight condition, carrying out reaction with hydrochloric acid in reaction tank to generate carbon dioxide for plant photosynthesis. The carbon dioxide in box is mixed with electrical fan, and light is replenished with daylight lamp, the temperature and humidity are reduced by using cooling tube, and water is added into cultivating disk with fluid infusion bottle. The plant marked with carbon- 13 or carbon- 14 is got after cultivation. The invention is characterized by simple and safe operation, prevention of carbon marked with carbon isotopes escape, increased utilization coefficient of carbon isotopes, suitability for carbon isotopes mark for various plants, especially for woody plant.

The invention discloses a method for detecting food pathogen by an electrochemical luminescence gene sensor on the basis of magnetic in-situ amplification. The method comprises the following steps: designing three PCR (Polymerase Chain Reaction) primers by using highly conserved DNA (deoxyribonucleic acid) sequence of food pathogen as a detection target segment; preparing a magnetic primer from the primer 1, preparing a TBR (tris (bipyridine) ruthenium) primer from the primer 2, and using the primer 3 as a common PCR primer, wherein when a target gene segment exists in the detection system, the magnetic primer can be amplified as the PCR process goes on, and the magnetic particles can directly have a TBR-modified double-chain nucleic acid product; when no target gene segment exists, the magnetic primer can not be amplified, and thus, no product with signal molecules can appear; and carrying out magnetic separation and in-situ electrochemical luminescence detection. The method can be used for quickly, sensitively, safely and specifically detecting the target gene segment, and is very suitable for popularization in the actual detection process.

The invention discloses a matrix modifying reagent for detecting content of lead and cadmium elements in whole blood, which comprises the following compositions: diammonium phosphate with the mass concentration of 0.5 to 0.6 percent, millesimal polyoxyethylene octyl phenyl ether with volume concentration of 2.0 to 4.0 percent, millesimal concentrated nitric acid with volume concentration of 1.0 to 3.0, and the balance of ultrapure water. The invention also provides a method for detecting content of lead and cadmium elements in the whole blood by the matrix modifying reagent, which dilutes concentration of the whole blood to be between one eighth and one thirteenth of the original concentration by adopting the matrix modifying reagent, and adds the diluted blood to an atomic absorption spectrometer for detecting. The detection method can detect lead and cadmium elements in one time, and has small amount of blood sample, simple operation, and low requirement on environment and operators.

The invention relates to a rainfall simulation experiment system in the technical field of civil engineering, and specifically discloses an experiment system for simulating rainfall-induced destruction of a reverse-order grain accumulation body, including an accumulation body model, a measurement system and a rainfall simulation device; the accumulation body model is composed of quartz The sand is piled up and positioned in the model groove, and the pile model is layered from top to bottom through the separation of colored spacer strips; the measurement system includes a laser displacement sensor, a wire-pull displacement sensor, A water content sensor, a pore water pressure sensor, an acoustic emission sensor, a processor and a PIV system; the rainfall simulation device includes a number of spray units arranged above the accumulation body model, a water tank for water storage, and a water tank connected to the spray unit and the PIV system. Between the tanks is a water pump for delivering rainwater to the sprinkler unit and a rainfall intensity control assembly for controlling the rainfall intensity. The invention can be used for rain-induced deformation and landslide tests of different slope models.

The invention relates to a method for adjusting the suspension clamp of an overhead lightning protection line and a buffer device, and belongs to the technical field of maintenance of overhead transmission lines. Technical solution: including front deck (1), front end (2), inner screw (3), ratchet wrench (4), middle screw (5), outer screw (6), rear end ( 7), rear clamping seat (8), front clamping nail (9), rear clamping nail (10), front clamping empty slot (11), front clamping bolt (12), rear clamping empty slot (14) and rear Clamp bolt (15); used to adjust the suspension clamp to loosen the U-shaped clip nut after the suspension clamp of the overhead lightning conductor is deflected, so as to prevent the sudden sliding of the lightning conductor to generate impact force. When the invention adjusts the suspension clamp of the overhead lightning protection wire, it prevents the sudden sliding of the lightning protection wire to generate impact force, causing the tower to shake violently, ensures the safety of maintenance, low cost, simple processing and manufacturing process, small size, light weight, simple operation method and safe use. Reliable and practical.

The invention discloses a whole blood matrix modifying reagent. The modifying reagent comprises the following compositions: 3 to 7 mass millesimal lanthanum chloride, 0.5 to 1.5 volume millesimal polyoxyethylene octyl phenyl ether, 0.5 to 1.5 volume millesimal concentrated nitric acid, and the balance of ultrapure water. The invention also provides a method for simultaneously detecting multielement by the whole blood matrix modifying reagent, which dilutes concentration of the whole blood to be between one twenty-sixth and one thirty-fifth of the original concentration by adopting the whole blood matrix modifying reagent, and installs a multielement simultaneous measuring atomic absorption device for detecting after standing the whole blood. The method can simultaneously detect multiple trace elements in the whole blood, and has good environmental applicability, high measuring accuracy and simple operation.

The invention discloses a construction method of a BALB / c-background age-dependent hepatitis B mouse model system and an application thereof. In the model, BALB / c-background replicating form HBV transgenic mice are hybridized with Rag-1 gene knockout mice in the same background, thereby screening adult and young Rag-1<- / -> mice expressing HBsAg. Mouse spleen lymphocytes from wild non-sensitized mice in the same background are transfused back to the adult and young mice, thereby constructing age-dependent acute hepatitis B and chronic hepatitis B mouse models. The model can be used for exploring the cellular and molecular mechanism of virus removal and persistent virus infection and searching a novel target point for immunotherapy of the hepatitis B, thereby screening an anti-hepatitis B medicine. The construction method of the model has good repeatability and significant stability and reliability of detection indices of virology and biochemistry and pathology. The method is reasonable in cost and has good application prospect.

The invention discloses an online device for rapidly detecting the quality indicators of a prepreg tape, and relates to a device for detecting the quality indicators of the prepreg tape. The invention is to solve the problem of time-consuming, energy-consuming and large reagent-consuming nature of the analysis results in the existing prepreg tape detection method. A first guide roll and a second guide roll are respectively fixed on a first bracket in a vertical manner; a third guide roll and the second guide roll are arranged vertically and fixedly connected with a support plate which is vertically fixed on the first bracket, a fourth guide roll and the third guide roll are arranged in a top and bottom staggered manner, a probe is arranged at one side of the prepreg tape to be tested, thehead part of the probe is opposite to the prepreg tape to be tested, a reflecting plate is arranged at the other side of the prepreg tape to be tested and opposite to the probe, the input end of the probe is connected with a near infrared spectrometer through optical fiber, and the near infrared spectrometer is connected with a computer. The invention can acquire the quality information of the prepreg in a online real-time manner, rapidly reflects the changes of each quality indicator in the prepreg tape to be tested and data detection and production process are carried out synchronously, andthe detection results are accurate.

The invention discloses a method for continuously separating and preparing functional lactoprotein in raw and fresh milk. The method mainly comprises the following steps: step one, separating and recovering milk fat by adopting a centrifugal method; step two, separating casein by combining a chymosin precipitation method with an acid precipitation method; step three, centrifuging a solution to obtain a supernatant being whey protein, carrying out microfiltration sterilization, and recovering permeate liquid for later use; step three, recovering a lactoferrin crude product by adopting an isoelectric point method; step three, recycling trapped fluid by an ultrafiltration method to obtain a crude immunoglobulin product; step five, removing lactose components from the ultrafiltration permeateto obtain an alpha-lactalbumin crude product; and step six, carrying out chromatographic purification on the alpha-lactalbumin crude product to obtain high-purity protein. According to the invention,the casein is separated by adopting the chymosin precipitation method combined with the acid precipitation method, so that the whey protein yield is increased; the subsequent separation of three different functional lactoproteins is facilitated; the protein crude product and the high-purity protein can be respectively obtained; and the requirements of the food industry on different raw materials are met.

The invention discloses a method for preparing a porous material from oil shale ash.The method comprises the steps that the dry oil shale ash is added into a sodium hydroxide solution, heating is performed until the temperature is increased to 50 DEG C-70 DEG C, stirring is performed for 4-6 hours, the temperature is regulated to 80 DEG C-90 DEG C, stirring is continuously performed for 6-8 hours, and oil shale ash turbid liquid is formed; the turbid liquid is put into an ultrasonic grinder to be subjected to the ultrasonic effect for 1-2 hours, a liquid phase is obtained through solid-liquid separation, hydrotalcite is added into the liquid phase, stirring is continuously performed for 4-6 hours, aging is performed for 24-48 hours, and a solid phase is obtained through filtering and separating; the solid phase is washed and dried, calcining is performed for 3-5 hours at the temperature of 300 DEG C-500 DEG C, the solid phase is cooled until the temperature of the liquid phase is lowered to room temperature, and then the porous material is obtained.According to the method, the waste resources are fully utilized, the raw material sources are rich, the operation method is simple and safe, and the prepared material is wide in application range and can serve as a catalyst carrier and an absorbing material.

An electric operating device for a high-voltage handcart of an electric high-voltage switchgear, including a mechanical cart and an adjustable support balance frame, the mechanical cart and the adjustable support balance frame are connected by an adjustable screw; the bottom of the mechanical cart is equipped with a pulley and a power supply The adjustable supporting balance frame is provided with a motor, the output shaft of the motor is connected with the rotating shaft transmission rod, and the end of the rotating shaft transmission rod is provided with a replaceable slotted head. The operation method of the invention is simple and safe, the operation is convenient and convenient to move, and the speed is uniform when the electric operation handcart switch is performed, which meets the requirements of the switch manufacturer. When centralized overhaul test and new installation and commissioning of multiple high-voltage handcart switches, high-intensity continuous and frequent operations can be performed in a short period of time, with low loss, high economic benefits, safety, and labor-saving operators.