Method and device for plants carbon isotope labelling

Abstract

The invention discloses a method and device for marking plant carbon isotopes. The method comprises following steps: sowing seed in the cultivating disk which located in the soil; putting cultivating disk into air- tight box; adding sodium carbonate marked with carbon-14 or carbon- 13 under air- tight condition, carrying out reaction with hydrochloric acid in reaction tank to generate carbon dioxide for plant photosynthesis. The carbon dioxide in box is mixed with electrical fan, and light is replenished with daylight lamp, the temperature and humidity are reduced by using cooling tube, and water is added into cultivating disk with fluid infusion bottle. The plant marked with carbon- 13 or carbon- 14 is got after cultivation. The invention is characterized by simple and safe operation, prevention of carbon marked with carbon isotopes escape, increased utilization coefficient of carbon isotopes, suitability for carbon isotopes mark for various plants, especially for woody plant.

Description

technical field [0001] The invention relates to the technical field of isotope tracer, more specifically to a plant carbon isotope labeling method, and also relates to a plant carbon isotope labeling device. The invention is suitable for researchers in institutions of higher learning and scientific research units to carry out carbon-14 or carbon-13 isotope labeling and tracing research on plants. Background technique [0002] Due to its high sensitivity, isotope tracer technology is called another great invention after the invention of microscope technology, and has been widely used in various fields of biology research. In soil science research, soil organic matter is not only an important material basis for soil fertility, but also plays an important role in maintaining soil aggregate structure and water retention and ventilation performance. Research on the cycle and accumulation of soil organic carbon is important for maintaining soil fertility and sustainable agricultur...

AI Technical Summary

Problems solved by technology

[0003] In the 1960s, British soil scientist Jenkinson designed a device for producing carbon-14 isotope-labeled plants, but it was complicated and inconvenient to operate, and the pressure control method in the box had defects, especially when adding When hydrochloric acid and carbon-14 labeled sodium bicarbonate, it is difficult to ensure the prevention of leakage due to the violent reaction

In addition, because the device uses a liquid culture method for plant production, it is not only easy to breed non-target organisms such as algae, but also may be different from plants grown in the soil, and some woody plants are even difficult to grow normally in a liquid culture solution; and the method Also not suitable for studying the amount of organic matter carbon exuded by plant roots

Many researchers in China usually use the intermittent labeling method, that is, plants in soil pots are grown under natural air environment conditions, and the plants are moved into the labeling box at regular intervals for carbon isotope labeling for one day. This method also has difficulties in strictly controlling labeling. The leakage of carbon dioxide gas is also not suitable for studying the amount of organic carbon secreted by plant roots, and the labeling intensity of carbon isotopes in the labeled plant materials is not uniform, so it is not suitable for studying the organic carbon of labeled plants in soil. decomposition and transformation

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