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Stem cell preparation for beautifying and resisting aging as well as preparation method and application of stem cell preparation

A stem cell preparation and stem cell technology, applied in the biological field, can solve the problems of short maintenance time and single effect, achieve strong plasticity, improve filling effect, and repair skin defects

Pending Publication Date: 2021-01-08
西安九州再生医学集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a stem cell preparation for beauty and anti-aging, aiming at solving the problem of simple injection of hyaluronic acid or joint injection of hyaluronic acid and different cells proposed in the background technology. The problem of short maintenance time and single effect

Method used

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  • Stem cell preparation for beautifying and resisting aging as well as preparation method and application of stem cell preparation
  • Stem cell preparation for beautifying and resisting aging as well as preparation method and application of stem cell preparation
  • Stem cell preparation for beautifying and resisting aging as well as preparation method and application of stem cell preparation

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Experimental program
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Effect test

Embodiment 1

[0046] This embodiment provides a stem cell preparation for beauty and anti-aging, which includes component A and component B. Specifically, the preparation method of the stem cell preparation includes the following steps:

[0047] S1. Weigh a certain amount of medium molecular weight hyaluronic acid for injection, prepare a 0.8% hyaluronic acid solution with normal saline, store it in a refrigerator at 4°C in the dark and allow it to dissolve naturally. It will take about 72 hours until it is completely dissolved. .

[0048] S2. Acquisition of platelet concentrate growth factor (PCGF) and serum:

[0049] (1) Draw 8 mL of autologous blood into a blood collection tube without anticoagulant added, put the blood collection tube into a centrifuge and balance it.

[0050] (2) Set up the centrifugation program, and the centrifugation equipment will obtain stratified samples through differential centrifugation steps of: acceleration 30sec, 2700r / min 2min, 2400r / min 4min, 2700r / min 4...

Embodiment 2

[0070] This embodiment provides a stem cell preparation for beauty and anti-aging, which includes component A and component B. Specifically, the preparation method of the stem cell preparation includes the following steps:

[0071] S1. Weigh a certain amount of medium-molecular-weight hyaluronic acid for injection, prepare a 0.6% hyaluronic acid solution with normal saline, store it in a refrigerator at 4°C in the dark and allow it to dissolve naturally. It will take about 72 hours until it is completely dissolved. .

[0072] S2. Acquisition of platelet concentrate growth factor (PCGF) and serum:

[0073] (1) Draw 10 mL of autologous blood into a blood collection tube without anticoagulant added, put the blood collection tube into a centrifuge and balance it.

[0074] (2) Set up the centrifugation program, and the centrifugation equipment will obtain stratified samples through differential centrifugation steps of: acceleration 30sec, 2700r / min 2min, 2400r / min 4min, 2700r / min ...

Embodiment 3

[0094] This embodiment provides a stem cell preparation for beauty and anti-aging, which includes component A and component B. Specifically, the preparation method of the stem cell preparation includes the following steps:

[0095] S1. Weigh a certain amount of medium molecular weight hyaluronic acid for injection, prepare a 0.5% hyaluronic acid solution with normal saline, store it in a refrigerator at 4°C in the dark and let it dissolve naturally. It will take about 72 hours until it is completely dissolved. .

[0096] S2. Acquisition of platelet concentrate growth factor (PCGF) and serum:

[0097] (1) Draw 9 mL of autologous blood into a blood collection tube without anticoagulant added, put the blood collection tube into a centrifuge and balance it.

[0098] (2) Set up the centrifugation program, and the centrifugation equipment will obtain stratified samples through differential centrifugation steps of: acceleration 30sec, 2700r / min 2min, 2400r / min 4min, 2700r / min 4min, ...

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Abstract

The invention is applicable to the technical field of biology, and provides a stem cell preparation for beautifying and resisting aging as well as a preparation method and application of the stem cellpreparation. The stem cell preparation comprises a component A and a component B, wherein the component A comprises platelet concentration growth factors; the component B comprises umbilical cord mesenchymal stem cells, placenta polypeptide, serum and a hyaluronic acid solution; the mass of the placenta polypeptide is 8%-10% of that of the hyaluronic acid solution; and the mass of the serum is 20%-35% of that of the hyaluronic acid solution. The stem cell preparation provided by the invention has the advantages of easiness in angiogenesis and tissue regeneration after injection, high survivalrate of transplanted cells and the like, can eliminate facial wrinkles, improve skin texture and brighten skin color, and is good in repair effect and long in maintenance time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a stem cell preparation for beauty and anti-aging, its preparation method and application. Background technique [0002] At present, hyaluronic acid is commonly used in facial micro-shaping in the beauty industry. As one of the most popular injection materials, hyaluronic acid is convenient, effective, quick to take effect, and can effectively solve various wrinkles. However, after hyaluronic acid is injected into the skin, it will be slowly metabolized and disappear over time, and the metabolic rate varies from person to person, and generally lasts for about 4 to 6 months. Specifically, there are two main factors affecting the metabolism of hyaluronic acid: on the one hand, thinner people absorb faster after injecting hyaluronic acid than obese people; The relatively young skin after acid metabolism is faster, and the maintenance time is correspondingly shorter. The da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/08A61K47/36C12N5/0775A61P17/16A61P17/18A61K35/16A61K35/19A61K35/51
CPCA61K38/18A61K35/51A61K35/19A61K35/16A61K38/012A61K9/0019A61K9/08A61K47/36A61P17/18A61P17/16C12N5/0665C12N2509/00C12N2509/10A61K2300/00
Inventor 凡孝菊赵莹何婷婷聂苏秦田丽军王黎明朱艳丽
Owner 西安九州再生医学集团有限公司
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